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Offlinecookeman
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How long will poured agar last?
    #7921031 - 01/23/08 06:19 PM (16 years, 9 days ago)

I was wondering how long poured agar will stay viable? Assuming everything was sterile and the procedure went as planned. I pour almost step fo step the same way RR does in his dvd's. Same flow hood, same petri's, same agar mix etc. So let's just say how long will agar's petri's stay viable? I'm wondering because it would be a lot more convenient to pour a 100 or so over a few days and be able to use them for a couple weeks rather than have to prep 25 petri's every time I need them.

P.S. I wrap my petri's in parafilm as well.


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OfflineDoctorRobert
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Re: How long will poured agar last? [Re: cookeman]
    #7921038 - 01/23/08 06:21 PM (16 years, 9 days ago)

Well, some master cultures on agar are kept for months in the fridge.


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OfflineRogerRabbitM
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Re: How long will poured agar last? [Re: DoctorRobert]
    #7921948 - 01/23/08 09:19 PM (16 years, 9 days ago)

If you're slipping them back into the sleeve and sealing it up, you can leave them for a month or two at room temperature. Beyond that time, they'll be too dry to use.
RR


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Offlinecookeman
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Re: How long will poured agar last? [Re: RogerRabbit]
    #7925401 - 01/24/08 05:41 PM (16 years, 8 days ago)

So would it be better to put them in the fridge while they're not in use, or leave them at room temperature?


--------------------
“Let’s put it this way – to lump psychedelic mushrooms into the same group as methamphetamine is like lumping the Bible into the same group as Mein Kampf.
I mean shit; they’re both books, right?”

Joe Rogan


R.I.P. - "Bones" - One of the greatest people I've ever had the pleasure of getting to know and become friends with.


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OfflineRogerRabbitM
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Re: How long will poured agar last? [Re: cookeman]
    #7925450 - 01/24/08 05:48 PM (16 years, 8 days ago)

Either one. I keep mine at room temp. Expect to lose a few to penicillium in storage, even if slipped back into the sleeve. Temperature changes cause air to breath in and out of the sleeve, and mold spores float along.
RR


--------------------
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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
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Offlineflushme
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Re: How long will poured agar last? [Re: RogerRabbit]
    #7925618 - 01/24/08 06:18 PM (16 years, 8 days ago)

i got pre poured ones along time from mycopath and i stored the 1s i dident use in the fridge for a few weeks.

what about after you pour them, you seal with parafilm before storing? RR?


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