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Offlineflushme
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g2g from multispore
    #7912818 - 01/21/08 11:00 PM (16 years, 11 days ago)

so what do you guys think about doing g2g transfers from multispore, and not isolates?


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InvisibleBlutjager
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Registered: 06/11/06
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Re: g2g from multispore [Re: flushme]
    #7912819 - 01/21/08 11:02 PM (16 years, 11 days ago)

Although I have never done it personally it sounds like it would work just fine


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Offlineflushme
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Re: g2g from multispore [Re: Blutjager]
    #7912853 - 01/21/08 11:07 PM (16 years, 11 days ago)

ive done it once before i just would like to know others thoughts on it. my only concern is that it would end up being a non fruiting or poorly fruiting substrain.


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Invisibleohmatic
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Re: g2g from multispore [Re: flushme]
    #7913456 - 01/22/08 01:20 AM (16 years, 10 days ago)

its allright


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Offlinexaxphaanes
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Re: g2g from multispore [Re: ohmatic]
    #7913559 - 01/22/08 02:02 AM (16 years, 10 days ago)

it's ok because so many strains are pretty good fruiters in multispore despite what some people think i have maybe 1 in every 10-15 jars turn out to be bad fruiters in multispore just make sure to get the best looking jar.


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Offlineflushme
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Re: g2g from multispore [Re: xaxphaanes]
    #7913567 - 01/22/08 02:08 AM (16 years, 10 days ago)

ill always use the 1st jar fully colonized in whatever batch as it would be the fastest and i think most contam resistant


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Offlinexaxphaanes
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Re: g2g from multispore [Re: flushme]
    #7913640 - 01/22/08 03:02 AM (16 years, 10 days ago)

mycelium doesnt resist contams it grows with it.now the fastest should be ok since contams are less likely to contam a fully colonized substrate since there isnt any food for it to grow


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Offlinehazey
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Registered: 01/20/08
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Re: g2g from multispore [Re: flushme]
    #7914230 - 01/22/08 11:10 AM (16 years, 10 days ago)

i always do g2g, it works for me. i usually use a sterile spoon to transfer 2-3 pieces of colonized rye to the uncolonized jar. really small transfers have shown to work better though they take longer.


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Offlineautographguys
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Re: g2g from multispore [Re: hazey]
    #7914262 - 01/22/08 11:19 AM (16 years, 10 days ago)

I do multi spore g2g. I inoculate 8 wbs jars with a 10 cc syringe.. I like to use the most aggressive jars. The ones that colonize the fastest. For the transfer. I have been doing this for the past 4 months with great success.


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InvisibleBUDDHA_702
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Registered: 02/17/07
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Re: g2g from multispore [Re: autographguys]
    #7914593 - 01/22/08 12:59 PM (16 years, 10 days ago)

It works good for me, I just use the best looking jar I have for the G2G.


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Offlinehazey
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Re: g2g from multispore [Re: BUDDHA_702]
    #7914615 - 01/22/08 01:02 PM (16 years, 10 days ago)

nice avatar...


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Invisibledshak66
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Registered: 09/18/06
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Re: g2g from multispore [Re: BUDDHA_702]
    #7915844 - 01/22/08 05:21 PM (16 years, 10 days ago)

Quote:

BUDDHA_702 said:
It works good for me, I just use the best looking jar I have for the G2G.


Yep, about one out of every ten of mine are contaminated, but I can live with that. Works fine.


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