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Offlineismokeweed
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how many jars should a 10 cc syrings noc up?
    #7908165 - 01/21/08 01:23 AM (16 years, 12 days ago)

1/2 pint brf.

i just inoculated nine jars and it took 8 cc's.
did i go a bit overboard?


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OfflineUCX_911
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Re: how many jars should a 10 cc syrings noc up? [Re: ismokeweed]
    #7908166 - 01/21/08 01:24 AM (16 years, 12 days ago)

dont think so. I noc'ed up 6 jars with 10cc.


--------------------
"Reefer Madness."


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Offlineatomicblue
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Re: how many jars should a 10 cc syrings noc up? [Re: UCX_911]
    #7908175 - 01/21/08 01:29 AM (16 years, 12 days ago)

I did 10 jars with a 10 cc. Had plenty, so gave the extra to a couple in the same lot. The ones with a little extra are colonizing much faster. a little extra seemed to work out great but too wet is no good. Discretion and patience I'm learning.


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Offlineismokeweed
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Re: how many jars should a 10 cc syrings noc up? [Re: UCX_911]
    #7908177 - 01/21/08 01:29 AM (16 years, 12 days ago)

ok cool. well shit. did i do too little?


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Offlineismokeweed
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Re: how many jars should a 10 cc syrings noc up? [Re: ismokeweed]
    #7908183 - 01/21/08 01:31 AM (16 years, 12 days ago)

i know if you go overboard it can speed it up a little but can be wasteful. i reckon if i didn't do enough then i just fucked myself.


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OfflineCubie
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Re: how many jars should a 10 cc syrings noc up? [Re: ismokeweed]
    #7908185 - 01/21/08 01:33 AM (16 years, 12 days ago)

Sounds fine dude.

You shoulda made an LC though and then you could have done a lot more.


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Offlineismokeweed
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Re: how many jars should a 10 cc syrings noc up? [Re: ismokeweed]
    #7908188 - 01/21/08 01:34 AM (16 years, 12 days ago)

ok good deal. I said the two before this before i read yours atomic blue.


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Offlineatomicblue
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Re: how many jars should a 10 cc syrings noc up? [Re: ismokeweed]
    #7908205 - 01/21/08 01:42 AM (16 years, 11 days ago)

Not too little. The others in my batch are moving slower but are still fine, as I'm sure yours will be.


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Invisibletahoe
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Re: how many jars should a 10 cc syrings noc up? [Re: ismokeweed]
    #7909119 - 01/21/08 10:56 AM (16 years, 11 days ago)

Quote:

ismokeweed said:
1/2 pint brf.

i just inoculated nine jars and it took 8 cc's.
did i go a bit overboard?



have you read the mmgg or the pf tek?


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OfflineMykokrazy
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How to inoculate 100s of jars with one syringe [Re: ismokeweed]
    #7919641 - 01/23/08 12:21 PM (16 years, 9 days ago)

Just take a 10 cc syringe and boil a quarter pint jar of water. (Mke sure theres a hole and tape to cover the hole. Anyways after boiling the quarter pint jar full of water. Inject your syringe into the solution. You now have about 10 full syringes. (Dont worry about dilution, It's worked great for me. Ill post a tek and pics soon.


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Invisiblesmokedout420
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Re: How to inoculate 100s of jars with one syringe [Re: Mykokrazy]
    #7919729 - 01/23/08 12:44 PM (16 years, 9 days ago)

in that case why dont you just make a lc? you can go by this tek also instead of clonig you can do muti spore.
LC Cloning - Newbie friendly pictorial

ill look a little bit more and try and find you a lc tek that explains it multi spore way for you. not say this tek isnt good but so you can see what im talking about.


Edited by smokedout420 (01/23/08 01:03 PM)


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Invisiblesmokedout420
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Re: How to inoculate 100s of jars with one syringe [Re: smokedout420]
    #7919797 - 01/23/08 01:01 PM (16 years, 9 days ago)

here's what i was suggesting for you isw

Crystal Clear LC's the easy way


Edited by smokedout420 (01/23/08 01:04 PM)


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Offlinebagsofun
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Re: how many jars should a 10 cc syrings noc up? [Re: Cubie]
    #7920228 - 01/23/08 03:20 PM (16 years, 9 days ago)

Quote:

Cubie said:
Sounds fine dude.

You shoulda made an LC though and then you could have done a lot more.






I finally started listening to all the people telling me this....made an LC with a simple honey tek, now two weeks later I have a whole jar full of myc.. Definatly a good solution to me getting pissed at burning through the ready-made spore syringes.


Definatly reccomend making an LC....really easy and makes 100's of cc's of solution out of 2 cc's of spore solution.


--------------------
Ass, gas, or grass, nobody rides for free


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OfflineMykokrazy
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Can you inject water into a regular pf-tek jar and pull it back up to use as a mycelium syringe [Re: bagsofun]
    #7927149 - 01/24/08 10:16 PM (16 years, 8 days ago)

Can you inject water into a regular pf-tek jar and pull it back up, to use as a mycelium syringe?


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Invisibletahoe
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Re: Can you inject water into a regular pf-tek jar and pull it back up to use as a mycelium syringe [Re: Mykokrazy]
    #7927593 - 01/24/08 11:35 PM (16 years, 8 days ago)

it might work but it is too risky with a pf jar. The pf jars are not sealed from outside contams and you would want to break the mycelium up first which would be tough with a pf jar


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


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I need to proofread


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OfflineScavengerType
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Re: how many jars should a 10 cc syringes noc up? [Re: ismokeweed]
    #7927915 - 01/25/08 12:51 AM (16 years, 8 days ago)

I usually do 1cc per jar that's 2.5ml per inoc points. When I looked it up on the techs from this sight while I was wondering myself, I believe the given range was from .5cc(1ml) per jar to 2.5cc(25ml) so obviously there's not much of an upper limit.

I'm intrigued from these LCM techs there are a lot of AG supply stores where I could load up on sterile syringes so I'd like to ask a simple question. Is LCM good after refrigeration and how long?


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Offlineveda_sticks
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Re: how many jars should a 10 cc syringes noc up? [Re: ScavengerType]
    #7928679 - 01/25/08 06:47 AM (16 years, 7 days ago)

8cc's for 9 jars is good. you can use anywhere from .5cc to 2cc per jar.

Obviously the more spores you have in your susbtrate the faster they will collinise, but.. the more spores the more varience in genetics and the more chance that not all spores will be able to mate and form into one organism (which can inhibit fruiting).

LC's are great, but there is 1 disadvantage, telling a contaminated LC apart from a healthy is in most cases impossible. If you are confident with your sterile technique then go for LC's. I wouldnt recommend it to most beginners for that reason. Very discouraging when you think you got some good LC and knock up some jars to have them mould on you.


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek


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Offlinerizingfire
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Re: how many jars should a 10 cc syringes noc up? [Re: veda_sticks]
    #7928749 - 01/25/08 07:25 AM (16 years, 7 days ago)

Too much spore solution can slow your project, LC's are good but it is hard to tell with some bacterias however with trich and other stuff it is relatively easy to tell. Patience is key! I noc up about 10-11 jars per syringe.


--------------------
aka NHMI


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Offlinebagsofun
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Re: Can you inject water into a regular pf-tek jar and pull it back up to use as a mycelium syringe [Re: Mykokrazy]
    #7928892 - 01/25/08 08:37 AM (16 years, 7 days ago)

Quote:

Mykokrazy said:
Can you inject water into a regular pf-tek jar and pull it back up, to use as a mycelium syringe?




I tried that twice with colonized grain-bags only to get tons of green mold on the newly knocked up grain bags. I was very clean/careful but I guess I still managed to eff up somethin....


--------------------
Ass, gas, or grass, nobody rides for free


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OfflineMykokrazy
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Re: Can you inject water into a regular pf-tek jar and pull it back up to use as a mycelium syringe [Re: bagsofun]
    #7967178 - 02/01/08 11:24 PM (16 years, 2 hours ago)

If any body else has tried this please post a comment. I just dont see why it wont work.


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