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LearJet
Stranger
Registered: 01/11/07
Posts: 44
Last seen: 14 years, 9 months
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agar to grain or grain to grain?
#7905270 - 01/20/08 10:31 AM (16 years, 12 days ago) |
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I am getting ready to inoculate some quart jars of WBS and I am trying to decide how I am going to do it. I have 2 jars left over from the last time that the bottom of the jars failed to colonize. I have conceded that they are never going to and gave up on them. The upper 2/3's of the jars are colonized fully. I also have several colonized agar petri's that were made from the last grow as well. Should I do a grain to grain transfer into the new jars or should I do agar to grain? What are the pros/cons of each?
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DJYoshaBYD


Registered: 04/07/07
Posts: 2,405
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Re: agar to grain or grain to grain? [Re: LearJet]
#7905303 - 01/20/08 10:40 AM (16 years, 12 days ago) |
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really, its your choice... you can either isolate a good chunk off of your dishes, or you can get a chunk of mycelium from your jar... I guess it just depends on what you are trying to do.. are you trying to isolate a strain, or are you just growing mushies? either way, you should get growth... lol... like I said, its really just depending on what you are trying to do..
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RogerRabbit
Bans for Pleasure



Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 3 days
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Re: agar to grain or grain to grain? [Re: DJYoshaBYD]
#7905407 - 01/20/08 11:15 AM (16 years, 12 days ago) |
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Quote:
Should I do a grain to grain transfer into the new jars or should I do agar to grain? What are the pros/cons of each?
Neither.
Since the bottom of the grain jars didn't colonize, something is wrong. Don't use them for grain to grain transfers.
A petri dish that is fully colonized is not one that I'd use to inoculate grain masters with, even if the dish was wrapped with parafilm. I'd suggest taking a small piece of mycelium from the petri dishes, and use it to inoculate fresh dishes. Allow that to grow for a few days, and then grab a very tiny piece of mycelium from the leading edge of the new growth. Transfer that fresh mycelium to a third dish and allow to grow 2/3 of the way across the dish. This clean mycelium can then be used to inoculate up to ten 1 quart/liter grain masters, which can each inoculate ten more via grain to grain transfers.
I avoid taking the uncolonized agar when I make the transfers by outlining the mycelium growth with my scalpel so I only take what's covered with mycelium, and none at all from the very edges of the petri dish, where contaminant spores may have entered and be laying dormant. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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Roadkill
Retired Shroomery Mod



Registered: 12/11/01
Posts: 22,674
Loc: Montana
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Re: agar to grain or grain to grain? [Re: LearJet]
#7905426 - 01/20/08 11:20 AM (16 years, 12 days ago) |
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do what Roger suggested with the agar...
then you will have an isolate.
this doesn't mean that you will get good fruits though...
you won't know that til you grow some out.
it's sorta hit and miss til you get a good isolate going...
then when you have a good one, make a master and keep it.
tc
-------------------- Laterz, Road Who the hell you callin crazy? You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch! Brainiac said: PM the names with on there names, that means they have mushrooms for sale.
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LearJet
Stranger
Registered: 01/11/07
Posts: 44
Last seen: 14 years, 9 months
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Re: agar to grain or grain to grain? [Re: RogerRabbit]
#7905433 - 01/20/08 11:21 AM (16 years, 12 days ago) |
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I should have probably mentioned that the petri's I have are the fourth in a series of dishes I was isolating a strain on. I have already done as you suggested. These petri's have just been hanging out sealed up in the fridge. The jars that did not colonize were made wrong, I tried to rush a grow and messed up the moisture content of the jars. The bottom of the jars are like cement. The new jars I have are made correctly and look to be perfect moisture.
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LearJet
Stranger
Registered: 01/11/07
Posts: 44
Last seen: 14 years, 9 months
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Re: agar to grain or grain to grain? [Re: LearJet]
#7905942 - 01/20/08 02:09 PM (16 years, 12 days ago) |
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well, I stuck agar wedges in them and put them in my incubator. Now the waiting begins again.
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