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InvisibleWWorker
...

Registered: 09/09/07
Posts: 395
considering trying out some agar...please help.
    #7902193 - 01/19/08 03:24 PM (16 years, 13 days ago)

I am considering making the move to agar. It seems to be an axtremely versatile media and the skills are highly useful. I just don't really know where to start.

According to the Psilocybin Mushroom Handbook,(I also have RRs videos but a friend has borrowed them so I will probably hold off until I have them in hand), senescence is a real and valid concern. I don't want this to happen to me or my cultures, but I don't want to make my own agar media completely from scratch. Is it possible to just buy a premixed agar recipe and add different grains to them?

Am I on the right track there?

Can someone recommend a good general agar recipe to work with. It looks like sporeworks has the widest selection. Overwhelmingly so....

Thanks in advance.


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OfflineCitizenKind
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Registered: 05/27/07
Posts: 17
Loc: Colorado
Last seen: 10 months, 6 days
Re: considering trying out some agar...please help. [Re: WWorker]
    #7902211 - 01/19/08 03:32 PM (16 years, 13 days ago)



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InvisibleWWorker
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Registered: 09/09/07
Posts: 395
Re: considering trying out some agar...please help. [Re: CitizenKind]
    #7902246 - 01/19/08 03:46 PM (16 years, 13 days ago)

Quote:

CitizenKind said:
I have used this tek with success.
http://www.shroomery.org/forums/showflat.php/Cat/0/Number/6613630/an/0/page/0
CitizenKind,




thanks for the link. Seems cheap and easy enough to at least learn the ropes and maybe even stick with permanently. If I am transfering the same strain multiple times senescense will become and issue right? Could I substitute, or just add different grain flours to the potato flakes?

Thanks again.


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Invisibletahoe
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Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
Trusted Cultivator
Re: considering trying out some agar...please help. [Re: WWorker]
    #7903024 - 01/19/08 07:10 PM (16 years, 13 days ago)

Quote:

WWorker said:
senescence is a real and valid concern.




This is unlikely to happen. if you start from multispore and transfer 2-3 times away from that you will be able to an aggresive growing and fruiting strain. You will have to go well beyond to start worrying



Quote:

WWorker said:
Is it possible to just buy a premixed agar recipe and add different grains to them?





Why would you add different grains to the agar? Buy a premixed one like you said. Maybe get the antibiotic agar from fp or check to see if sporeworks had one.

Do you have a pressure cooker? Do you have a glove box? Do not attempt agar without these.
get this
http://sporeworks.com/store/catalog/Intermediate-Agar-Culturing-Kit-p-16212.html


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Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread


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InvisibleWWorker
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Registered: 09/09/07
Posts: 395
Re: considering trying out some agar...please help. [Re: tahoe]
    #7903091 - 01/19/08 07:24 PM (16 years, 13 days ago)

Tahoe,

My eventual hope is to be able to clone wilds and culture wild prints which i assume will take some isolating. Because of this, I may have to subculture several times. Am I wrong in assuming this?

I would vary the grain flour to avoid senescence as per instruction in the Psilocybin Mushroom Handbook. I am also asking if this is a necessity in the first place.

I do own a pressure cooker and glove box, and my sterile procedures have not failed me thus far.

Thanks for the advice


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Invisibletahoe
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Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
Trusted Cultivator
Re: considering trying out some agar...please help. [Re: WWorker]
    #7903232 - 01/19/08 07:53 PM (16 years, 13 days ago)

okay it all makes sense now. You have a little more knowledge than most here.
Cloning wilds is not hard but they are dirty.
As for starting cultures from spores you might need to do a few isolation to find something good,\

Dont worry about changing up the media too much. You could order 2 types of agar, wheat and malt.
I like to start my wild clones and prints on antibiotic mea and than i put them to wheat agar. So it changes it a bit


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread


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OfflineRogerRabbitM
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Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 3 days
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Re: considering trying out some agar...please help. [Re: WWorker]
    #7903255 - 01/19/08 07:58 PM (16 years, 13 days ago)

You don't need to vary the agar recipe. I've used MEA for 20+ years on all my cultures, and they're fine. Eating the same food doesn't cause senescence, cell divisions do.

When working with wild prints, I'll often use antibiotic agar, and mix it with less malt. If you buy pre-mixed agar powder from fp or sporeworks, you can mix it a bit weaker than recommended. This will keep from feeding the mold and bacteria quite so much.

When you get the video back, watch the section on strain isolation. It demonstrates the best places to take transfers.
RR


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Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


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InvisibleWWorker
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Registered: 09/09/07
Posts: 395
Re: considering trying out some agar...please help. [Re: RogerRabbit]
    #7903360 - 01/19/08 08:19 PM (16 years, 13 days ago)

RR and Tahoe...thanks for the additional advice...and the compliment tahoe..lol


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