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Offlinetoytac
Stranger
Registered: 01/17/08
Posts: 38
Last seen: 13 years, 7 months
Several Rye Casings failed on me!!! please help!
    #7893158 - 01/17/08 01:49 PM (16 years, 2 months ago)

Hey Guys

im pretty new to this and really am excited about that first flush that i will get.. but it hasnt happened yet!!

I have gotten all the parts complete such as performing sterile activities, inoculation, grain to grain transfer (to keep a constant supply of colonizing jars.

HERES MY PROBLEM! :
i am casing them in a disposable cake pan, and casing with 50/50 sphagnum peat moss, and verm. it is then incubated for a couple days at 86, no overlay has been detected/ cold shocked for 15 hrs in the fridge, then maintained 73-76 degree 90-95 rh.... i HAVE HAD 3 DIFFERENT CASINGS FAIL... they never pin.. i fan 3 times daily.. everything is right.

PLEASE HELP! ive been failing for over 2 months now! what am i doing wrong for the last step!!???

thanks everyone

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Offlineendepth
The world remains ever the same.


Registered: 12/25/07
Posts: 647
Loc: Cloud 9
Last seen: 3 years, 8 months
Re: Several Rye Casings failed on me!!! please help! [Re: toytac]
    #7893242 - 01/17/08 02:17 PM (16 years, 2 months ago)

Snap some macro pics of those bad boys.


--------------------
Being normal is nothing to aspire to. It is something to get away from.


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Offlinegaladar


Registered: 05/09/07
Posts: 981
Loc: in a monotub!
Last seen: 2 years, 4 months
Re: Several Rye Casings failed on me!!! please help! [Re: endepth]
    #7893267 - 01/17/08 02:27 PM (16 years, 2 months ago)

Did you pasteurize the casing?

also, don you think 86 might be a bit too high. casings generate thier own heat. who knows what the internal temp might be, possible near 90.

and
higher temps=bacteria growth.

i would never incubate a casing at that temp.

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Offlinetoytac
Stranger
Registered: 01/17/08
Posts: 38
Last seen: 13 years, 7 months
Re: Several Rye Casings failed on me!!! please help! [Re: galadar]
    #7893300 - 01/17/08 02:39 PM (16 years, 2 months ago)

my last two failed casings never got infected but just took 20 days for me to decide they werent gonna pin

i will get some pics up tonight.

i use a closet storage rack with levels.. and a heater at the bottom. each level is a 5 degree diff from above and below.. like the bottom is 75 middle is 80 etc... my casings are toward the middle. so they are all ready prepared for internal heat!

ive even let them overlay.. to putting them in there with a few ridges peeking through the casings.. ive tried it several ways.. (SAME OUTCOME) those stubborn lil guys just wont pin..

thanks! il get the pics up later on tonight

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OfflineDharmaBum
keep thyreligion to thyself
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Registered: 08/04/06
Posts: 81
Last seen: 15 years, 3 months
Re: Several Rye Casings failed on me!!! please help! [Re: toytac]
    #7893306 - 01/17/08 02:41 PM (16 years, 2 months ago)

My friend above me is correct. No need to run your casing at 86. For one contams will come quicker at higher temp, and also the internal heat like my man said might be too high. They will keep themselves just fine at room temp. I prefer it actually. I only incubate petri dishes. Just some advice here too.... Forget the cold shock...waste of time with cubes. Also with the casings let them run until you see bits of mycelium coming up through to greet you. Patch the areas where it comes up with more casing media until the mycelium is coming up across the casing evenly. You want to insure a good pinset.
good luck


--------------------
Emancipate yourself from mental slavery
None but ourselves can free our minds....
B.M.

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OfflineDharmaBum
keep thyreligion to thyself
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Posts: 81
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Re: Several Rye Casings failed on me!!! please help! [Re: galadar]
    #7893329 - 01/17/08 02:50 PM (16 years, 2 months ago)

Sorry man overlooked the part where you already told him about the higher temps= bacteria growth. Didnt mean to repeat.


--------------------
Emancipate yourself from mental slavery
None but ourselves can free our minds....
B.M.

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InvisibleBlutjager
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Registered: 06/11/06
Posts: 9,220
Re: Several Rye Casings failed on me!!! please help! [Re: galadar]
    #7893353 - 01/17/08 02:58 PM (16 years, 2 months ago)

Quote:

galadar said:


i would never incubate a casing at that temp.




Me either,and skip the cold shock as well,it only slows things down

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Offlinegaladar


Registered: 05/09/07
Posts: 981
Loc: in a monotub!
Last seen: 2 years, 4 months
Re: Several Rye Casings failed on me!!! please help! [Re: DharmaBum]
    #7893450 - 01/17/08 03:24 PM (16 years, 2 months ago)

Quote:

DharmaBum said:
My friend above me is correct. No need to run your casing at 86. For one contams will come quicker at higher temp, and also the internal heat like my man said might be too high. They will keep themselves just fine at room temp. I prefer it actually. I only incubate petri dishes. Just some advice here too.... Forget the cold shock...waste of time with cubes. Also with the casings let them run until you see bits of mycelium coming up through to greet you. Patch the areas where it comes up with more casing media until the mycelium is coming up across the casing evenly. You want to insure a good pinset.
good luck




Quote:

Blutjager said:
Quote:

galadar said:


i would never incubate a casing at that temp.




Me either,and skip the cold shock as well,it only slows things down




right on. 5 shrooms for ya both

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Offlinedonpatch
purukogi...purukogi...


Registered: 11/28/07
Posts: 183
Last seen: 13 years, 1 month
Re: Several Rye Casings failed on me!!! please help! [Re: galadar]
    #7893483 - 01/17/08 03:32 PM (16 years, 2 months ago)

you did use lime in your peat moss casing right? and you sifted out all the woody shit?


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InvisibleCrasher
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Re: Several Rye Casings failed on me!!! please help! [Re: toytac]
    #7893497 - 01/17/08 03:35 PM (16 years, 2 months ago)

Quote:

toytac said:
ive even let them overlay.. to putting them in there with a few ridges peeking through the casings.. ive tried it several ways.. (SAME OUTCOME) those stubborn lil guys just wont pin..




How did you conclude that overlay was a good idea?
Has this all been the same strain?

it is possible to have a mycelial network that doesn't produce fruitbodies, right?


--------------------
Give me silence, water, hope;
Give me struggle, iron, volcanoes...

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Offlinetoytac
Stranger
Registered: 01/17/08
Posts: 38
Last seen: 13 years, 7 months
Re: Several Rye Casings failed on me!!! please help! [Re: Crasher]
    #7894135 - 01/17/08 05:53 PM (16 years, 2 months ago)

yes it has all been the same strain so far . is it possible that i have a bad strain?








this last pic is of the casing that has been sitting for approx 2 days now.. its the next one. and i want to make it work!



this pic is the set up with diff temp levels.. the fl lights are covered but they are exposed now just for the pic


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Offlinetoytac
Stranger
Registered: 01/17/08
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Last seen: 13 years, 7 months
Re: Several Rye Casings failed on me!!! please help! [Re: toytac]
    #7896913 - 01/18/08 10:36 AM (16 years, 2 months ago)

up

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Offlinegaladar


Registered: 05/09/07
Posts: 981
Loc: in a monotub!
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Re: Several Rye Casings failed on me!!! please help! [Re: toytac]
    #7896992 - 01/18/08 11:00 AM (16 years, 2 months ago)

mist it and report in 2 days.

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Offlinerizingfire
Mycoticus psychoticus
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Re: Several Rye Casings failed on me!!! please help! [Re: galadar]
    #7897001 - 01/18/08 11:02 AM (16 years, 2 months ago)

Could it be a CO2 issue? I have had a couple cakes never fruit but out of a batch of 80 there were 3. That would suck to have a shitload of trays of retard myc but I doubt that is the case.


--------------------
aka NHMI

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Offlinetoytac
Stranger
Registered: 01/17/08
Posts: 38
Last seen: 13 years, 7 months
Re: Several Rye Casings failed on me!!! please help! [Re: rizingfire]
    #7901765 - 01/19/08 01:32 PM (16 years, 2 months ago)

not sure if its a co2 issue.. i do give them proper fanning .i feel dumb because the first casing i ever put out had pins and the window was left open and it was the only night this year that 2 inches of snow fell... needless to say the pins were tiny craters in the white network the next morning.. SIGH...... it will happen..

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Offlinethedoc8
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Re: Several Rye Casings failed on me!!! please help! [Re: toytac]
    #7904850 - 01/20/08 07:14 AM (16 years, 2 months ago)

Why are your tubs covered in the picture if you are fruiting..?

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Offlinetoytac
Stranger
Registered: 01/17/08
Posts: 38
Last seen: 13 years, 7 months
Re: Several Rye Casings failed on me!!! please help! [Re: thedoc8]
    #7906204 - 01/20/08 03:46 PM (16 years, 2 months ago)

what do you mean "covered" ? the bottom pic was of jars and my casing before the fruiting process.. the fc wasnt in any of the pics and its being 12/12 with 2 flrsnt cabinet lights.

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Offlinetoytac
Stranger
Registered: 01/17/08
Posts: 38
Last seen: 13 years, 7 months
Re: Several Rye Casings failed on me!!! please help! [Re: toytac]
    #7910015 - 01/21/08 03:04 PM (16 years, 2 months ago)

ok guys.. i misted and its been a bit over 2 days.. I THINK THESE ARE PINS? YA??? i hope!

if these are pins.. i have it at 97 rh 77 degrees and fanning 3 times a day..

how long till dinner is served?






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OfflineYossarian22
Stranger
Registered: 09/12/07
Posts: 415
Last seen: 9 years, 3 months
Re: Several Rye Casings failed on me!!! please help! [Re: toytac]
    #7910232 - 01/21/08 04:04 PM (16 years, 2 months ago)

It seems like all of your trays are enclosed with little Fresh Air Exchange. Try either poking holes in the big tubs and filling them with polyfill or micropore tape or just open them up. Works better than fanning IME.

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Offlinetoytac
Stranger
Registered: 01/17/08
Posts: 38
Last seen: 13 years, 7 months
Re: Several Rye Casings failed on me!!! please help! [Re: Yossarian22]
    #7912360 - 01/21/08 09:37 PM (16 years, 2 months ago)

the big tray is poked and has lots of holes for fae

once pins have started, what is the growth rate of shrooms at their optimal fruiting conditions?

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Invisiblezyxwvutsr
rstuvwxyz


Registered: 12/30/07
Posts: 141
Re: Several Rye Casings failed on me!!! please help! [Re: toytac]
    #7912495 - 01/21/08 10:02 PM (16 years, 2 months ago)

double in size every 24 hours approx


--------------------
Cube Growth Parameters

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Invisiblejoshua m
Not a NoOb
Male

Registered: 07/14/07
Posts: 168
Loc: MiD wEsT
Re: Several Rye Casings failed on me!!! please help! [Re: toytac]
    #7912571 - 01/21/08 10:16 PM (16 years, 2 months ago)

That last pic is contamination i believe


--------------------
We can't stop here...This is bat country!!!

Fear and Loathing is the greatest movie ever!!!

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Invisiblejoshua m
Not a NoOb
Male

Registered: 07/14/07
Posts: 168
Loc: MiD wEsT
Re: Several Rye Casings failed on me!!! please help! [Re: joshua m]
    #7912574 - 01/21/08 10:17 PM (16 years, 2 months ago)

How do you pasturize your casing mix


--------------------
We can't stop here...This is bat country!!!

Fear and Loathing is the greatest movie ever!!!

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Offlinearloniousmonk
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Registered: 01/01/08
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Re: Several Rye Casings failed on me!!! please help! [Re: toytac]
    #7912591 - 01/21/08 10:19 PM (16 years, 2 months ago)

You need more fresh air I think


--------------------
They mostley come at night,... mostley.....

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OfflineCenebite
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Registered: 10/27/04
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Re: Several Rye Casings failed on me!!! please help! [Re: toytac]
    #7912991 - 01/21/08 11:32 PM (16 years, 2 months ago)

That yellow on the bottom pic doesn't look good...

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Offlinexaxphaanes
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Registered: 08/08/05
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Re: Several Rye Casings failed on me!!! please help! [Re: Cenebite]
    #7913435 - 01/22/08 01:14 AM (16 years, 2 months ago)

the yellow could be two things either it got burned or kinda scorched from the light or it is metabolites both really aren't too bad but if you have allot of it, it could mean bacterial contamination


--------------------
"Anything i say is fictional"
  what you should look for in manure

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Offlinetoytac
Stranger
Registered: 01/17/08
Posts: 38
Last seen: 13 years, 7 months
Re: Several Rye Casings failed on me!!! please help! [Re: toytac]
    #7915435 - 01/22/08 04:20 PM (16 years, 2 months ago)

do anyones pins grow in a lower temp? such as 70-74?

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Invisibledurban_poison
myco contractor

Registered: 09/19/01
Posts: 2,417
Re: Several Rye Casings failed on me!!! please help! [Re: toytac]
    #7915519 - 01/22/08 04:32 PM (16 years, 2 months ago)

they will grow in those temps. i have the same shelving.

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Invisibledurban_poison
myco contractor

Registered: 09/19/01
Posts: 2,417
Re: Several Rye Casings failed on me!!! please help! [Re: toytac]
    #7915555 - 01/22/08 04:37 PM (16 years, 2 months ago)

moisture in your casing looks like the issue. those arent brazil or pf are they? fae should also be increased.

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Offlinetoytac
Stranger
Registered: 01/17/08
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Last seen: 13 years, 7 months
Re: Several Rye Casings failed on me!!! please help! [Re: durban_poison]
    #7919912 - 01/23/08 01:36 PM (16 years, 2 months ago)

well here is the thing... i was very unorganized in my first attempt which was two separates - pink b and taz

one of them died out due to contamination a while back.. and i dont know which one i have . i did get another syringe of b+ and it will be a couple more days until i inoculate a few more jars of it!

which is the easiest to grow in your opinion? fastest? more giving?

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