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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation > Mushroom Cultivation Archive > Panaeolus
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Panaeolis trop on horse poo. (8-13) *completed*
    #789252 -

This will be a grow log featuring Panaeolis tropicalis. I know the OMC has been questioning the authenticity of prints of this species in circulation, but until I know what it really is, I will continue to call it Pan trop (cyan?)

Dude has attempted to grow this species 3 or 4 times, all of which yielded in lots of contams, and 1 tiny fruit. Hopefully this time things will be different. We will see...

Anyway, the grow log will include a tray of microwave pasteurised horse manure, that will be spawned with a jar of multispore innoc?d Pan trop (cyan?) colonised millet.

--
7/31

This jar of colonized birdseed was used to spawn a tray of microwave pasteurised horse poo.



this is about 12 hours after spawning.



----
update 8-1

twelve hours later.



----
8-2



***this is strange, I know i updated earlier and checked it, and it ws fine, then later the update is gone...???***

---
udated 8-4

dude will case tomorrow morning. He will need to put the casing in the fridge (after 12 hours more of incubation) for the next few days because there is no space for the tray in his fruiting chamber. After he takes it out of the fridge it will go right into the fruiting chamber with more updates then...

----
update 8-8

after 3 days in the fridge, a spot opened up in the fruiting chamber. So dude took it out of the fridge, sprinkled a very small amount of casing over a few areas, sprayed, then put into pinset conditions.

Here is a pic of it resting in the chamber:



----
update 8-10

today dude saw a bunch of knots scattered throughout the casing. Here is a closeup:



it shouldn?t be too long now.

---
update 8-11



very tiny, but lots of them. Hopefully they will get stockier, because they look pretty petite. This is the best dude has done with this species, so he?s happy nomatter what!

-----
update 8-13

Here is a pic from a little while ago:



Dude said he was too tired to pic the fruits which are ripe now, and will wait till morning and he will take some better pics then too.

They are growing well, i guess, but they are soo damn small. I?ve seen other posts which show nice good sized specimens. Maybe a good clone needs to be picked...

----
morning



evening



They are actually fairly nice in size now. I am very happy to see dude get a flush like this, especially after all of the problems they?ve posed in the past, Finally!





--------------------
Study the past...
See the future...

Edited by Olgualion (08/18/02 10:38 AM)

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Re: Panaeolis tropicalis (cyan?) on nuked horse poo. [Re: Olgualion]
    #789256 -

Looks like you have a good shot at some success there!
keep up the posts!
pretty!

--Seril


--------------------
Seril,
Political language -true of all political parties- is designed to make lies sound truthful and murder respectable, and to give solidity to pure wind..

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Re: Panaeolis tropicalis (cyan?) on nuked horse poo. [Re: Olgualion]
    #789793 -

updated


--------------------
Study the past...
See the future...

Edited by Olgualion (08/01/02 04:55 AM)

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Re: Panaeolis tropicalis (cyan?) on horse poo. (8-1) [Re: Olgualion]
    #791909 -

updated 8-2


--------------------
Study the past...
See the future...

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Re: Panaeolis tropicalis (cyan?) on horse poo. (8-1) [Re: Olgualion]
    #792472 -

So far so good, I'd like to see how these turn out.


You seem to be having a lot of success with pasturization of dung using the microwave; just wondering if you could post your method for this.



--------------------
I saw you today on the number twelve bus,
you were going my way

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Re: Panaeolis tropicalis (cyan?) on horse poo. (8-1) [Re: Millencolin]
    #792616 -

Hi Millencolin,

Actually, it is fairly simple. First dude goes out to a horse trail in a local park, armed with a few empty plastic bags. He walks the trail in search of horse doo. He fills up the plastic bags with it, he tries to separate the doo into different bags depending upon freshness. When he gets home, he lays the poo out to dry in their respective piles.

Once a pile is completely dry, he brings it inside and begins breaking it up as finely as possible into a microwave safe bowl. After he has a good amount in the bowl, he then adds boiling water to the bowl. It doesnt matter if you add too much. this is allowed to sit for a half hour or so. He then puts the bowl in the microwave for like 5 minutes or so on high. removes the bowl and mixes up the dung. Puts it back into the microwave and nukes for a final 8 minutes on high. As soon as it stops he places some AL foil on top of the bowl, but leaves it in the micro (with the door closed) to cool overnight.

In the morning, he washes out an AL pan and then begins squeezing the dung a handful at a time to remove much of the water and puts it into the pan. After he has squeezed out all of the dung, he spawns it with the desired colonized substrate.

The black poo water left over is used to feed his many houseplants.

I think that covers everything, if i left anything out, or you have any more q?'s, feel free to ask..


--------------------
Study the past...
See the future...

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Re: Panaeolis tropicalis (cyan?) on horse poo. (8-2) [Re: Olgualion]
    #793652 -

I cant wait to see this.. I love your logs


--------------------
-SumGuy :cool:

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Re: Panaeolis tropicalis (cyan?) on horse poo. (8-1) [Re: Olgualion]
    #795905 -

Thanks for the info, I'll have to try this sometime soon and see how it turns out overall. Have you noticed any negative aspects as compared to other methods of pasturization?


--------------------
I saw you today on the number twelve bus,
you were going my way

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Re: Panaeolis tropicalis (cyan?) on horse poo. (8-1) [Re: Millencolin]
    #796375 -

updated up top...

The only negative that I can think of is that you can't pasteurise as much in a small microwave as you can in a pillowcase in a big pot

This is only dude's second attempt with this method, so it hasn't really been done enough to be able to tell for sure...


--------------------
Study the past...
See the future...

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Re: Panaeolis tropicalis (cyan?) on horse poo. (8-2) [Re: Olgualion]
    #803954 -

updated up top...


--------------------
Study the past...
See the future...

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Re: Panaeolis tropicalis (cyan?) on horse poo. (8-8) [Re: Olgualion]
    #808702 -

updated up top.


--------------------
Study the past...
See the future...

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Re: Panaeolis tropicalis (cyan?) on horse poo. (8-10) [Re: Olgualion]
    #810692 -

updated up top..


--------------------
Study the past...
See the future...

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Re: Panaeolis tropicalis (cyan?) on horse poo. (8-11) [Re: Olgualion]
    #810893 -

What is your secret of allways getting such an even pinset?

Do you have like 10 identical trays and allways only photograph the nicest?

I never have such a good pinset....


--------------------
Grow Mushrooms from Spores - PF-Tek for Simple Minds

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Re: Panaeolis tropicalis (cyan?) on horse poo. (8-11) [Re: Anno]
    #810895 -

To show you what I?m talking about:

I spawned 3 different panaeolus cyanescens strans to pasteurized cow dung the same day you did.
Mine look like this right now:

#

The 2 vermiculite trays are P. cubensis.

As you see, the pinset is not nearly as nice here.....


--------------------
Grow Mushrooms from Spores - PF-Tek for Simple Minds

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Re: Panaeolis tropicalis (cyan?) on horse poo. (8-11) [Re: Anno]
    #813616 -

He tries to match the enviromental conditions as closely as possible, he can read his casing very well IMO. I know you have both of these down.

But what I think a very important thing in the process he follows is the cold shock. Every tray he does gets covered with Al foil and holes poked in. Then when the first areas of mycelium poke through these areas are covered with more casing and sprayed. put back into incubation, then ususally less than 24 hours later they go into the fridge. dude always puts them into a clean trashbag first. They are left in the fridge for at least 12 hours, sometimes as long as a week(depending). This tray was in the fridge for 2 or 3 days i think. Then they are placed directly into the fruiting chamber, with initiation conditions.

This is what works for dude. And I stopped preaching about cold shocking a while ago.. lol

But anyway, I would much rather have your flush anyway. Your's are definitely bigger healthier and overall nicer looking!


--------------------
Study the past...
See the future...

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Re: Panaeolis tropicalis (cyan?) on horse poo. (8-13) [Re: Olgualion]
    #813810 -

I don't think they look all that small. They look pretty tasty to me anyways. I believe the Pan-Cyans are my favorite shrooms that I've ever seen cultivated. There's just something about the way they look... Either way, congratulations and enjoy the fruits of your labors.


--------------------
peace
bluefoot

"There is more in us than we know. If we can be made to see it, perhaps, for the rest of our lives, we will be unwilling to settle for less." - Kurt Hahn

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Re: Panaeolis tropicalis (cyan?) on horse poo. (8-11) [Re: Olgualion]
    #814003 -

I found that they don?t like a high humidity after the pins are there.

They grow much more naturraly looking and tall at 80% RH than at 90 or even more...


--------------------
Grow Mushrooms from Spores - PF-Tek for Simple Minds

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Re: Panaeolis tropicalis (cyan?) on horse poo. (8-11) [Re: Anno]
    #814184 -

Hmmm. Dude will have to play with his setup some so he can drop his humidity that low. The way he has it, when the tape is covering the holes in his sterlite, the humidity is about 95, and after tape removal, it drops to a little under 90. So he'll tweak it a bit for the next flush.


--------------------
Study the past...
See the future...

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Re: Panaeolis tropicalis (cyan?) on horse poo. (8-11) [Re: Olgualion]
    #814197 -

Gee somebody must think dude is a crappy cultivator! rating jumped from 5 to 3 in one vote (5+5+1)/3 = 3. They voted me a 1.. oh well. G.S.--> XcCtomlk!@2


--------------------
Study the past...
See the future...

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Re: Panaeolis tropicalis (cyan?) on horse poo. (8-11) [Re: Olgualion]
    #814222 -

Okay, so you cold shock for a few days and you recase when you see stuff poke up though the casing layer? Is that all you do, no secret dance or prayer?
I think your very talented, I rated you a 5 btw...
lurker

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Re: Panaeolis tropicalis (cyan?) on horse poo. (8-11) [Re: Anno]
    #814269 -

I've been thinking about the pinsets. I think that knowing your strain or isolate is very important. If you have a strain that shows too much variability with multispore, then it is important to make an isolate. Proper maintenance of the tray before initation is very important. Water content and patching are most important now. Dude keeps his casing often beyond field capacity, but always fans vigorously after a spraying. you want to patch any areas of mycelium that are dense. If you have more than a tiny bit of mycelium at each patch, you want to sprinkle some casing. Then when there are a bunch of areas of a very little mycelium showing it is time for initiation. It is now time for initiation or coldshock. whichever you prefer. This is going to be different for strains that take longer to pin. The B+ strain dude has seems to pin (i believe it is 4 days after initiation begins).

When initiation starts, I think the air exchanges are the most imortant factor. This is why dude leaves his coolmiost on 24-7. He doesn't want to just worry about the humidity, at the expense of air exchanges. So what he does is hold the air exchanges constant and use another method to reduce humidity. He uses holes in his terrarium. Pretty much randomly placed around the terrarium, covered during initiation to keep the humidity very high. Then he takes the tape off to reduce the humidity, during growth.

Heat actually seems to be the least important factor (again, for this specific strain). With this method dude often gets nice flushes. He has some pink buffalos going now, but they are not following this regimen well at all. The determining factor can often be the strain.



--------------------
Study the past...
See the future...

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Re: Panaeolis tropicalis (cyan?) on horse poo. (8-11) [Re: Olgualion]
    #816686 -

updated again last night.


--------------------
Study the past...
See the future...

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Re: Panaeolis tropicalis (cyan?) on horse poo. (8-11) [Re: Olgualion]
    #816876 -

>I am very happy to see dude get a flush like this

I can imagine this....


--------------------
Grow Mushrooms from Spores - PF-Tek for Simple Minds

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Re: Panaeolis tropicalis (cyan?) on horse poo. (8-11) [Re: Anno]
    #825393 -



This is better than the 1 mushroom he got last time! lol

He was only able to get was a few caps to drop a small bit of spores. And the tray is gone.. It had a little green on it after harvest. But he has another 4 or 5 cc's of multispore left.

Any tips for getting these guys to drop nice prints? Best time?

** I'm going to mark the log complete **


--------------------
Study the past...
See the future...

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Re: Panaeolis tropicalis (cyan?) on horse poo. (8-11) [Re: Olgualion]
    #829208 -

I've gotta say, I'm very, very, impressed! Looks like they've turned out even better than would have hoped. Congratulations.


--------------------
I saw you today on the number twelve bus,
you were going my way

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Re: Panaeolis tropicalis (cyan?) on horse poo. (8-11) [Re: Anno]
    #837611 -

Man those stems are shinny, looks like there's a wave of silver in there. They may be skinny but thats an amazing pin-set!!!!

Maybe i missed it(probably) but what was the casing layer made of? Are you going to give us a 2nd flush update?

5 mushrooms for you!
Fd


--------------------
___________________________________________________________________
Fd3000 is really a brown dancing monster. He uses the info he gets from his crazy dreams to help those in need. Too bad he doesn't really exist...

"I could walk up to the president and blow smoke in his stupid monkey face and all he could do is stand there grooving on it" - Homer Simpson

Edited by Fd3000 (08/23/02 06:24 AM)

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation > Mushroom Cultivation Archive > Panaeolus
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