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tahoe
Noob Slayer



Registered: 11/26/03
Posts: 6,274
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The Only LC Tech! The Liquid Culture How To Guide 3
#7887928 - 01/16/08 11:36 AM (16 years, 16 days ago) |
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I see so many questions about lc's. I see all the different recipes, the techs, the ideas. I have seen recipes that use honey, karo, dextrose, turbino... I know these ideas came up because of the ease of obtaining some of these products. We all have the Internet so its about time you guys slow down, order some real supplies, and make some real liquid cultures that work great.
I have tried most of the formulas and lid designs and this seems to be the best one.
The formula is basic. 1 gram of powdered brewers malt per 500ml of water. Any water will work. Tap, spring, distilled, rain, toilet(unused). A gram of powdered malt is about a 1/2 teaspoon of powder. I know this will seem like its not enough but it is. It is more than enough. When making lc's less sugar is better. You do not want a hyper active mycelium that goes into shock once put to grain which has less sugar. You want the opposite. A healthy low sugar mycelium that goes hyper active once it hits the grain that has more sugar.
Here are the supplies you will need fo this.
Jars and plastic lids. metal work and are good but I know some of you will try to deviate from the tech and try to microwave the liquid. If you do not have a pressure cooker then you are not serious about mycology.
Quart jars with plastic lids. I prefer small mouth but either will work http://www.canningpantry.com/ball-quart-mason-jar.html http://sporeworks.com/store/product.php?productid=16254&cat=0&page=2
brewers malt. Buy the lightest color that they offer. http://sporeworks.com/store/catalog/Dry-Malt-Extract-Wheat-Formula-p-16218.html
Rubber stoppers http://www.sciplus.com/search.cfm
1/4th and 1/2 inch drill bits. Preferably forstner http://www.traditionalwoodworker.com/product_info.php/cPath/269_270_277/products_id/1412
Whatman filters Also known as Syringe filters
 http://cgi.ebay.com/Corning-Syringe-Filters_W0QQitemZ270200001056QQihZ017QQcategoryZ26415QQssPageNameZWDVWQQrdZ1QQcmdZViewItem
Silicon http://doitbest.com/main.aspx?pageid=64&sku=779324&memberid=0129&associate=true
Drill a 1/4 inch hole and a 1/2 hole in the lid. Drill the holes towards the outside of the lid but do not go through the area that makes the seal on the jar. Lightly sand the area around the hole so that the silicon will stick. Use just a bit a silicon and glue the stopper and filter to the lid. Wait 24 hours. Maybe take the dirt bike out for soil eroding, nature destroying, air polluting, noise producing ride.

Now add your 500ml of water(any type) and 1 dry gram of powder to the quart jar. Smaller or larger jars will require more/less water/malt. You guys should be smart enough to figure it out but than again you guys never cease to amaze me.
Put the lid on and cover with foil. Pc for 20-30 mins at 10-15 psi. 30 mins will be more than enough at 5 psi so don't get carried away here like I know you guys love to do. Longer is not better. You will cause floaters and might damage the sugar but this is highly unlikely. Let cool in your pc, glove box, or flow hood. Use the injector port for spore syringe inoculations although this is not recommended. It is best as with any lc to use a live culture from a petri dish. Always keep the lid covered with foil when not in use. Do not get carried away and use coffee filters. These things harbor mold spores. Always spray the jar/lid with alcohol before unscrewing the lid to drop in the culture. Always wipe the injector port with alcohol before inserting a needle. I am becoming a big fan of the alcohol wipes and holding them around the needle while inserting it and pulling it out.
Here are the lids with the correct location of the holes. The stopper needs a 1/2inch hole. The filter needs anywhere from a 1/8th-1/4 inch hole

The injector port with silicon on it

The filter with silicon

Just make sure the silicon oozes out from under the filter and injector port.

Fill the open area around the filter. You do not want an area for growth here because it will be impossible to clean. It will not affect the contam rate because if its contamed than the lc will be. It will be sterile after pc'ing.

It should look like this

After pc'ing they will be this color. They are actually a little lighter in color but the flash turned them yellow

After a few weeks. This is a fully colonized lc. It needs to be in the fridge. You will most likely be able to use your lc a week after inoculation. This one is basically over grown but still good.

Here is one in action. Look how is just stops spinning. This is because it is super thick. Like jello.

I would like to thank www.sporeworks.com for their years of support and help with pioneering radical and new cultivation ideas. I am trying to get them to stock these filters.
Credit for this tech shouldn't be given to me. I first saw the idea in one of monstermich's post. I have heard that the good man "Agar" first brought these filters to our attention.
I would also like to thank Samurai_Jim, blood4blood, oysterguy63, and emrandel for giving me the strength and courage to finish this tech.
Edited by tahoe (01/17/08 12:37 PM)
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Boomers420
Young Hand



Registered: 10/19/06
Posts: 439
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Re: The Only LC Tech! The Liquid Culture How To Guide [Re: tahoe]
#7887942 - 01/16/08 11:42 AM (16 years, 16 days ago) |
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God I love you Tahoe
-------------------- Fuck me, ain't no shrooms where I lives now
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ronue
steamin'
Registered: 05/27/07
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Re: The Only LC Tech! The Liquid Culture How To Guide [Re: Boomers420]
#7887991 - 01/16/08 12:02 PM (16 years, 16 days ago) |
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It still seems complicated - if you opt for internet shopping instead of using your supermarket, why not buy some YPD broth from known lab suppliers? I use Sigma's,
http://www.sigmaaldrich.com/catalog/search/ProductDetail/SIGMA/Y1375
and I was never dissapointed. Just mix the powder with water and autoclave . The price is very reasonable as well!!
As for tubes / containers, for LC, I buy falcon tubes - they can be autoclaved as well, and they come sterilised.
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Samurai_Jim
Samurai Emperor.


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Re: The Only LC Tech! The Liquid Culture How To Guide [Re: ronue] 1
#7888143 - 01/16/08 12:54 PM (16 years, 16 days ago) |
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Dang, I was just getting interested.
-------------------- Decreasing the world's bug population, one faceshield at a time.
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blood4blood
Calmer Than You Are


Registered: 04/25/07
Posts: 6,029
Loc: The Valley
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Re: The Only LC Tech! The Liquid Culture How To Guide [Re: Samurai_Jim]
#7888222 - 01/16/08 01:17 PM (16 years, 16 days ago) |
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finish your tek you bastard.... 
im listening.
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shroober
Myco Junkie


Registered: 01/02/08
Posts: 879
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Re: The Only LC Tech! The Liquid Culture How To Guide [Re: blood4blood]
#7888261 - 01/16/08 01:28 PM (16 years, 16 days ago) |
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at least i know where to get those syringe filters now
finish the tek!!!
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MrMaddHatter
Dementia praecox



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Re: The Only LC Tech! The Liquid Culture How To Guide [Re: tahoe]
#7888283 - 01/16/08 01:34 PM (16 years, 16 days ago) |
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Quote:
If you do not have a pressure cooker then you are not serious about mycology.
True
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fastfred
Old Hand



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Re: The Only LC Tech! The Liquid Culture How To Guide [Re: tahoe]
#7888314 - 01/16/08 01:42 PM (16 years, 16 days ago) |
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Quote:
tahoe said: I see so many questions about lc's. I see all the different recipes, the techs, the ideas. I have seen recipes that use honey, karo, dextrose, turbino... I know these ideas came up because of the ease of obtaining some of these products. We all have the interenet so its about time you guys slow down, order some real supplies, and make some real liquid cultures that work great.
LOL! That is too true.
As far as your LCs go... scientists have been doing this sort of thing for 100's of years. You're not going to find any 'improvements' that haven't already been tried. All of the so-called 'improvements' are nothing more than added complexity and cost. They simply lead to a larger chance of failure and added cost.
If you want the most simple and best technique that has been devised in the centuries and decades of scientific research then simply drill a hole in your lid and pull some polyfill through it.
Take any sort of solid lid, drill a hole in the lid (about pencil to dime size is best IME), take some polyfill and draw it out, then fold it in half, pull the loose ends through the hole, and trim any large excess off with scissors.
Optionally you can drill another hole large enough for your needle or cannula and tape it. Sometimes it's handy to have this extra hole near the edge for sucking up myc. Otherwise you can just use the polyfill hole.
Even the polyfill hole is really optional. All you really need is one hole to inject and retrieve from the jar. LCs don't need air exchange unless you want to grow them overly thick. If you do that you'll probably run into problems drawing up such thick myc.
-FF
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oysterguy63
60's child

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Re: The Only LC Tech! The Liquid Culture How To Guide [Re: tahoe]
#7888489 - 01/16/08 02:47 PM (16 years, 16 days ago) |
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Quote:
tahoe said:
ah nevermind, you guys wont listen anyways
Will too uh huh. 
Some of us are good grasshoppas Sensei
-------------------- Oysters...yuummmmm
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Samurai_Jim
Samurai Emperor.


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Re: The Only LC Tech! The Liquid Culture How To Guide [Re: oysterguy63]
#7888491 - 01/16/08 02:49 PM (16 years, 16 days ago) |
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I'll listen to about anything. Gwar, not so much, but just about anything.
-------------------- Decreasing the world's bug population, one faceshield at a time.
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emrandel
Love iS freeD


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Re: The Only LC Tech! The Liquid Culture How To Guide [Re: Samurai_Jim]
#7888599 - 01/16/08 03:15 PM (16 years, 16 days ago) |
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Well... I'll definitely be using some of those resources for my next LC's. Wish you'd finished the tek, it was shaping to be a good one.
Wish I'd known to use brewer's malt... we have a brew shop close by. Next time... next time...
-------------------- "No drug, not even alcohol, causes the fundamental ills of society. If we're looking for the source of our troubles, we shouldn't test people for drugs, we should test them for stupidity, ignorance, green and love of power!" - P.J. O'Rourke
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tahoe
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Re: The Only LC Tech! The Liquid Culture How To Guide [Re: emrandel]
#7888863 - 01/16/08 04:08 PM (16 years, 16 days ago) |
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okay, let me finish it. Look back to the first post
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
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blood4blood
Calmer Than You Are


Registered: 04/25/07
Posts: 6,029
Loc: The Valley
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Re: The Only LC Tech! The Liquid Culture How To Guide [Re: tahoe]
#7889487 - 01/16/08 06:16 PM (16 years, 16 days ago) |
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damn good write up tahoe. thanks for finishing it man.
off to the favorites.
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Samurai_Jim
Samurai Emperor.


Registered: 01/12/08
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Re: The Only LC Tech! The Liquid Culture How To Guide [Re: blood4blood]
#7889755 - 01/16/08 07:06 PM (16 years, 16 days ago) |
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Very interesting.
-------------------- Decreasing the world's bug population, one faceshield at a time.
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Mycodood



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Re: The Only LC Tech! The Liquid Culture How To Guide [Re: Samurai_Jim]
#7890209 - 01/16/08 08:36 PM (16 years, 16 days ago) |
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Those whatman's are too expensive and have no real value add, and it's already been done. I use polyfill like FF and if the LC goes bad I just use another drop of spore solution and make a new one.
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Blutjager
Inhuman


Registered: 06/11/06
Posts: 9,220
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Re: The Only LC Tech! The Liquid Culture How To Guide [Re: Mycodood]
#7890307 - 01/16/08 08:55 PM (16 years, 16 days ago) |
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" If you do not have a pressure cooker then you are not serious about mycology."
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tahoe
Noob Slayer



Registered: 11/26/03
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Re: The Only LC Tech! The Liquid Culture How To Guide [Re: Mycodood]
#7890491 - 01/16/08 09:37 PM (16 years, 16 days ago) |
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Quote:
Mycodood said: Those whatman's are too expensive and have no real value add,
Its just a much cleaner setup
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
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blood4blood
Calmer Than You Are


Registered: 04/25/07
Posts: 6,029
Loc: The Valley
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Re: The Only LC Tech! The Liquid Culture How To Guide [Re: tahoe]
#7890522 - 01/16/08 09:44 PM (16 years, 16 days ago) |
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Quote:
tahoe said:
Quote:
Mycodood said: Those whatman's are too expensive and have no real value add,
Its just a much cleaner setup
hells yea! once you become self-sufficient not having things that make this hobby more convienant is just pointless.
i purchased 6 of those special lc lids and will never go back to a poly filtered lid
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emrandel
Love iS freeD


Registered: 03/03/07
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Re: The Only LC Tech! The Liquid Culture How To Guide [Re: blood4blood]
#7891022 - 01/16/08 11:17 PM (16 years, 16 days ago) |
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Awesome, thanks a lot for finishing the write up. I'll be using it soon to make some LCs. I especially appreciate the links to everything I need for the tek. It'll make it much easier to get supplies.
Good day to you.
-------------------- "No drug, not even alcohol, causes the fundamental ills of society. If we're looking for the source of our troubles, we shouldn't test people for drugs, we should test them for stupidity, ignorance, green and love of power!" - P.J. O'Rourke
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tahoe
Noob Slayer



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Re: The Only LC Tech! The Liquid Culture How To Guide [Re: emrandel]
#7891367 - 01/17/08 12:30 AM (16 years, 16 days ago) |
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This would start pinning soon if it wasnt a species that required casing http://www.shroomery.org/forums/files/08-003/052619518-IMG_1423.jpg
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
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tahoe
Noob Slayer



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Re: The Only LC Tech! The Liquid Culture How To Guide [Re: Mycodood]
#7892857 - 01/17/08 12:39 PM (16 years, 15 days ago) |
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Quote:
Mycodood said: Those whatman's are too expensive and have no real value add,
I updated the tech with another link for syringe filters check ebay. This is a good deal http://cgi.ebay.com/Corning-Syringe-Filters_W0QQitemZ270200001056QQihZ017QQcategoryZ26415QQssPageNameZWDVWQQrdZ1QQcmdZViewItem
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Uns4ne
I Fail At Life




Registered: 10/17/07
Posts: 112
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Re: The Only LC Tech! The Liquid Culture How To Guide [Re: tahoe]
#7893221 - 01/17/08 02:12 PM (16 years, 15 days ago) |
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smokedout420
The EnlightenedOne



Registered: 12/16/07
Posts: 484
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Re: The Only LC Tech! The Liquid Culture How To Guide [Re: Uns4ne]
#8096859 - 03/03/08 10:09 AM (15 years, 10 months ago) |
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dohhhh..... anyone using the .45um's my dumbass wasnt paying attention and bought the 5 of the .45um will this be ok?
edit: im really a dumb ass just seen tahoe's link for .45um
thanks dude
Edited by smokedout420 (03/03/08 10:18 AM)
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tahoe
Noob Slayer



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Re: The Only LC Tech! The Liquid Culture How To Guide [Re: smokedout420]
#8096866 - 03/03/08 10:12 AM (15 years, 10 months ago) |
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i use both and I have not noticed a difference. They will be fine. The .2 are of corse better but not needed.
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
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smokedout420
The EnlightenedOne



Registered: 12/16/07
Posts: 484
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Re: The Only LC Tech! The Liquid Culture How To Guide [Re: tahoe]
#8161513 - 03/18/08 10:16 AM (15 years, 10 months ago) |
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man so i messed up the last night and was in a rush moving my incubator. my lc fell over and some juice started coming out the filter this morning. i used one my blank syringe to hook up to the filter to pull the juice off the filter. do you think it will wick contams....... man what was i thinkin i should've looked inside.
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tahoe
Noob Slayer



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Re: The Only LC Tech! The Liquid Culture How To Guide [Re: smokedout420]
#8161556 - 03/18/08 10:25 AM (15 years, 10 months ago) |
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its done, if the filter got wet with the sugary liquid it will contam. It is probably still good as of now but it will go to shit soon. Cook some jars today
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
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smokedout420
The EnlightenedOne



Registered: 12/16/07
Posts: 484
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Re: The Only LC Tech! The Liquid Culture How To Guide [Re: tahoe]
#8161588 - 03/18/08 10:31 AM (15 years, 10 months ago) |
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will the filter still be usable if i pc again?
if not it no big i got a 50 pack of syringe filters coming in sometime today
thanks
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tahoe
Noob Slayer



Registered: 11/26/03
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Re: The Only LC Tech! The Liquid Culture How To Guide [Re: smokedout420]
#8161640 - 03/18/08 10:41 AM (15 years, 10 months ago) |
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no, it would have to have all the lc washed out of it.
They make replacement disc that you can put in yourself.
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
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tahoe
Noob Slayer



Registered: 11/26/03
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Re: The Only LC Tech! The Liquid Culture How To Guide [Re: tahoe]
#9802114 - 02/15/09 12:11 PM (14 years, 11 months ago) |
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this thread is truly relevant
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
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houdinihar
Adventurous Learner


Registered: 09/12/08
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Re: The Only LC Tech! The Liquid Culture How To Guide [Re: tahoe]
#9802750 - 02/15/09 02:20 PM (14 years, 11 months ago) |
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tahoe, thanks for posting this again. i am using this info now.
houdinihar
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GetHyphae
Stranger


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Re: The Only LC Tech! The Liquid Culture How To Guide [Re: houdinihar]
#9891948 - 03/01/09 06:17 PM (14 years, 10 months ago) |
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what is the rubber stopper used for? is it self-healing?
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tahoe
Noob Slayer



Registered: 11/26/03
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Re: The Only LC Tech! The Liquid Culture How To Guide [Re: GetHyphae]
#9893479 - 03/01/09 10:05 PM (14 years, 10 months ago) |
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yeah, its an injection port
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
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ano
2-stepper

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Re: The Only LC Tech! The Liquid Culture How To Guide [Re: tahoe]
#9935988 - 03/08/09 08:52 PM (14 years, 10 months ago) |
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How many pc's/lc usages do you usually get out of one of these filters before it breaks down/gets too dirty/etc? How often do you replace your filters?
-------------------- Dream like you'll live forever. Live like you'll die tomorrow.
Edited by ano (03/08/09 08:53 PM)
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rocket88
Semi-Noob



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Re: The Only LC Tech! The Liquid Culture How To Guide [Re: tahoe]
#9981438 - 03/16/09 11:59 AM (14 years, 10 months ago) |
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This is a great tek. I just bought my first syringes and this is definitely something I'm gonna try but I have a question about something.
Could you use a poly Buchner funnel with Tyvek over the top instead of the Whatman filters? The Buchner should still fit into the hole in the lid and can be sealed w/ the silicone. A sheet of Tyvek or two rubber banded to the top of the funnel should allow for good air exchange and would keep the filter well away from any liquid.
Here's a link to the Buchner.
Thanks a lot for all of the great information you guys make available.
rocket
-------------------- "Rightful liberty is unobstructed action according to our will within limits drawn around us by the equal rights of others. I do not add 'within the limits of the law' because law is often but the tyrant's will, and always so when it violates the rights of the individual." - Thomas Jefferson
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No_Life_G33k
Now with 10%less noobness


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Re: The Only LC Tech! The Liquid Culture How To Guide [Re: Samurai_Jim]
#9983912 - 03/16/09 06:15 PM (14 years, 10 months ago) |
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Has anyone hads an issue where the whatman filters slow down the process when trying to asperate the solution out of the LC jar???
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Django12
Spider Pants ofLance McGance


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Re: The Only LC Tech! The Liquid Culture How To Guide [Re: No_Life_G33k]
#10577090 - 06/26/09 02:26 PM (14 years, 7 months ago) |
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Hate to resurrect this thread, and this might be a stupid question..But do you inject through the rubber stopper, and aspirate through the filter? or do you inject and aspirate through the rubber port? Thanks.
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CH HELL
Brain Sturgeon



Registered: 10/02/08
Posts: 6,610
Loc: mars
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Re: The Only LC Tech! The Liquid Culture How To Guide [Re: Django12]
#10577103 - 06/26/09 02:29 PM (14 years, 7 months ago) |
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Never stick a needle through the fliter! Every thing should be done through the injection port. CH
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Ayrios
Stranger

Registered: 12/08/02
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Re: The Only LC Tech! The Liquid Culture How To Guide [Re: tahoe]
#10754596 - 07/28/09 10:16 AM (14 years, 6 months ago) |
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I have a couple quart jars coming along very nicely at 9 days... I'm just wondering when I'll be able to use ALL of it. Currently, there are a couple dozen clusters of myc in each jar with a lot of tiny specks of myc. In the past, I haven't needed to use LCs right away, but now I have a bunch of in vitro bags I want to noc up. Even though there's only a bit of clustered myc in each jar, can I still agitate them, suck up 60 cc per bag, and noc them up? Would it be better to wait till it's much thicker?
And I don't feel bad about resurrecting this thread as it's seemingly the most complete LC tek I've read :P
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veda_sticks
Cultivator




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Re: The Only LC Tech! The Liquid Culture How To Guide [Re: Ayrios]
#10754620 - 07/28/09 10:20 AM (14 years, 6 months ago) |
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It would be better to wait until its ready.
In the meantime, inoc a few bags up with spores. It will serve as a backup incase the LC doesnt work out.
-------------------- PF TEK - writeup by EvilMushroom666 Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD Cakes can and will pin! - So you think cakes suck for pins. Your wrong Franks Simple Coir/Verm Tek Franks Proper Pasturisation Tek Franks Spawning To Bulk - Monotub Professor Pinheads RTV Injection Port Tek Foo Mans No Soak WBS Prep Tek
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Ayrios
Stranger

Registered: 12/08/02
Posts: 619
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Re: The Only LC Tech! The Liquid Culture How To Guide [Re: Ayrios]
#10754663 - 07/28/09 10:33 AM (14 years, 6 months ago) |
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Actually I'm working with sterile substrate bags (MM-style), so I need lots of LC :P I will just wait another week or so and see how it's looking then.
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busyskull
Captain Amigo



Registered: 07/01/09
Posts: 62
Loc: Tincan Mountain
Last seen: 12 years, 4 months
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Re: The Only LC Tech! The Liquid Culture How To Guide [Re: tahoe]
#10879963 - 08/18/09 12:39 AM (14 years, 5 months ago) |
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this teks awsome, at the comic store you can bye glass beads, and if you get a long neck glass bottle and drill a hole in the cap and fill with silicone , let dry and auticlave , it works good
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just me
Friend



Registered: 07/01/07
Posts: 9,745
Loc: IL/MO/FL/TX/HI/OR
Last seen: 4 years, 7 months
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Re: The Only LC Tech! The Liquid Culture How To Guide [Re: busyskull]
#10880277 - 08/18/09 01:32 AM (14 years, 5 months ago) |
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thank you tahoe
--------------------
  -------------------------------------------------- -pEaCeLoVeGoDbLeSs- "The Downfall of Mankind; is Believing He Has Limitations."
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wildrose
Learner

Registered: 02/09/10
Posts: 41
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Re: The Only LC Tech! The Liquid Culture How To Guide [Re: just me]
#12083339 - 02/23/10 01:10 PM (13 years, 11 months ago) |
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I was using the search feature to read up on the LC teks. This one looks great but there was one question I saw unanswered...sorry if the answer is elsewhere, but here goes:
How many uses can you get out of the Whatman filters? Also, if some Canadian can give me an Edmonton or Red Deer retailer of them that would be great. The ones I've found on ebay are really being SCALPED.
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Mystery420



Registered: 11/03/08
Posts: 791
Loc:
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Re: The Only LC Tech! The Liquid Culture How To Guide [Re: wildrose]
#12083369 - 02/23/10 01:18 PM (13 years, 11 months ago) |
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I like your yz 250:D Good guide too
-------------------- "I'm the one who's gonna die when it's time for me to die, so let me live my life the way I want." -Jimi Hendrix So fuck ya'll all of ya'll If ya'll don't like me blow me
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hamloaf
Loaf of Fam.


Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma.
Last seen: 1 year, 8 months
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Re: The Only LC Tech! The Liquid Culture How To Guide [Re: tahoe]
#12083660 - 02/23/10 02:17 PM (13 years, 11 months ago) |
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Bump
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SickDog
Boozenpottenkooter


Registered: 11/25/10
Posts: 73
Loc: Jersey Pines
Last seen: 2 months, 13 days
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Re: The Only LC Tech! The Liquid Culture How To Guide [Re: tahoe]
#15162844 - 10/01/11 02:56 PM (12 years, 3 months ago) |
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another great write-up with deleted pics
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Slayer1024
Stranger Shroomer



Registered: 03/08/11
Posts: 397
Loc: At his place ^^
Last seen: 9 years, 6 months
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Re: The Only LC Tech! The Liquid Culture How To Guide [Re: SickDog]
#15162954 - 10/01/11 03:27 PM (12 years, 3 months ago) |
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MattyBear
sharing is caring



Registered: 07/04/13
Posts: 204
Last seen: 3 years, 6 months
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Re: The Only LC Tech! The Liquid Culture How To Guide [Re: Slayer1024]
#18542950 - 07/11/13 07:58 AM (10 years, 6 months ago) |
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Great tek. I just received some pearl oyster and lions mane LC syringes and I was wondering if there is anyway to pasteurize a 5 gal bucket of the liquid so I can inoculate and have 5 gal for outside spray purposes?
-------------------- Here's what we can do to change the world right now, to a better ride. Take all the money we spend on weapons and defenses each year and instead, spend it feeding, clothing and educating the poor of the world, which it would pay for many times over , not one human being excluded, and we could explore space, together, both inner and outer, forever, in peace. ~Bill Hicks~ Looking to build up a print library
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MattyBear
sharing is caring



Registered: 07/04/13
Posts: 204
Last seen: 3 years, 6 months
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Re: The Only LC Tech! The Liquid Culture How To Guide [Re: MattyBear]
#18542959 - 07/11/13 08:04 AM (10 years, 6 months ago) |
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Also, you mention that it is best to use live culture from a petri dish, As I do not use agar, would a small amount of colonized WBS spawn be better than an LC syringe? If so how much should I use and would a glove box be the most sterile way of transferring WBS to the LC jars?
-------------------- Here's what we can do to change the world right now, to a better ride. Take all the money we spend on weapons and defenses each year and instead, spend it feeding, clothing and educating the poor of the world, which it would pay for many times over , not one human being excluded, and we could explore space, together, both inner and outer, forever, in peace. ~Bill Hicks~ Looking to build up a print library
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