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Invisibletahoe
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Registered: 11/26/03
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Re: The Only LC Tech! The Liquid Culture How To Guide [Re: Mycodood]
    #7892857 - 01/17/08 12:39 PM (16 years, 15 days ago)

Quote:

Mycodood said:
Those whatman's are too expensive and have no real value add,



I updated the tech with another link for syringe filters
check ebay. This is a good deal
http://cgi.ebay.com/Corning-Syringe-Filters_W0QQitemZ270200001056QQihZ017QQcategoryZ26415QQssPageNameZWDVWQQrdZ1QQcmdZViewItem


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InvisibleUns4ne
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Re: The Only LC Tech! The Liquid Culture How To Guide [Re: tahoe]
    #7893221 - 01/17/08 02:12 PM (16 years, 15 days ago)

:headbanger:


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Invisiblesmokedout420
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Re: The Only LC Tech! The Liquid Culture How To Guide [Re: Uns4ne]
    #8096859 - 03/03/08 10:09 AM (15 years, 10 months ago)

dohhhh..... anyone using the .45um's my dumbass wasnt paying attention and bought the 5 of the .45um will this be ok?

edit: im really a dumb ass just seen tahoe's link for .45um

thanks dude


Edited by smokedout420 (03/03/08 10:18 AM)


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Invisibletahoe
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Re: The Only LC Tech! The Liquid Culture How To Guide [Re: smokedout420]
    #8096866 - 03/03/08 10:12 AM (15 years, 10 months ago)

i use both and I have not noticed a difference. They will be fine. The .2 are of corse better but not needed.


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread


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Invisiblesmokedout420
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Re: The Only LC Tech! The Liquid Culture How To Guide [Re: tahoe]
    #8161513 - 03/18/08 10:16 AM (15 years, 10 months ago)

man so i messed up the last night and was in a rush moving my incubator. my lc fell over and some juice started coming out the filter this morning. i used one my blank syringe to hook up to the filter to pull the juice off the filter. do you think it will wick contams....... man what was i thinkin i should've looked inside.


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Invisibletahoe
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Re: The Only LC Tech! The Liquid Culture How To Guide [Re: smokedout420]
    #8161556 - 03/18/08 10:25 AM (15 years, 10 months ago)

its done, if the filter got wet with the sugary liquid it will contam. It is probably still good as of now but it will go to shit soon.
Cook some jars today


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread


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Invisiblesmokedout420
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Re: The Only LC Tech! The Liquid Culture How To Guide [Re: tahoe]
    #8161588 - 03/18/08 10:31 AM (15 years, 10 months ago)

will the filter still be usable if i pc again?

if not it no big i got a 50 pack of syringe filters coming in sometime today

thanks:thumbup:


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Invisibletahoe
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Re: The Only LC Tech! The Liquid Culture How To Guide [Re: smokedout420]
    #8161640 - 03/18/08 10:41 AM (15 years, 10 months ago)

no, it would have to have all the lc washed out of it.

They make replacement disc that you can put in yourself.


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread


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Invisibletahoe
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Re: The Only LC Tech! The Liquid Culture How To Guide [Re: tahoe]
    #9802114 - 02/15/09 12:11 PM (14 years, 11 months ago)

this thread is truly relevant


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread


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Offlinehoudinihar
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Re: The Only LC Tech! The Liquid Culture How To Guide [Re: tahoe]
    #9802750 - 02/15/09 02:20 PM (14 years, 11 months ago)

tahoe,
thanks for posting this again. i am using this info now.

houdinihar


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OfflineGetHyphae
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Re: The Only LC Tech! The Liquid Culture How To Guide [Re: houdinihar]
    #9891948 - 03/01/09 06:17 PM (14 years, 10 months ago)

what is the rubber stopper used for? is it self-healing?


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Invisibletahoe
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Re: The Only LC Tech! The Liquid Culture How To Guide [Re: GetHyphae]
    #9893479 - 03/01/09 10:05 PM (14 years, 10 months ago)

yeah, its an injection port


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread


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Offlineano
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Re: The Only LC Tech! The Liquid Culture How To Guide [Re: tahoe]
    #9935988 - 03/08/09 08:52 PM (14 years, 10 months ago)

How many pc's/lc usages do you usually get out of one of these filters before it breaks down/gets too dirty/etc? How often do you replace your filters?


--------------------
Dream like you'll live forever.
Live like you'll die tomorrow.


Edited by ano (03/08/09 08:53 PM)


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Offlinerocket88
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Re: The Only LC Tech! The Liquid Culture How To Guide [Re: tahoe]
    #9981438 - 03/16/09 11:59 AM (14 years, 10 months ago)

This is a great tek.  I just bought my first syringes and this is definitely something I'm gonna try but I have a question about something.

Could you use a poly Buchner funnel with Tyvek over the top instead of the Whatman filters?  The Buchner should still fit into the hole in the lid and can be sealed w/ the silicone.  A sheet of Tyvek or two rubber banded to the top of the funnel should allow for good air exchange and would keep the filter well away from any liquid.

Here's a link to the Buchner.

Thanks a lot for all of the great information you guys make available.

rocket


--------------------
"Rightful liberty is unobstructed action according to our will within limits drawn around us by the equal rights of others. I do not add 'within the limits of the law' because law is often but the tyrant's will, and always so when it violates the rights of the individual." - Thomas Jefferson

:crazy2:


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OfflineNo_Life_G33k
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Re: The Only LC Tech! The Liquid Culture How To Guide [Re: Samurai_Jim]
    #9983912 - 03/16/09 06:15 PM (14 years, 10 months ago)

Has anyone hads an issue where the whatman filters slow down the process when trying to asperate the solution out of the LC jar???


--------------------
Hot Water Pasteuriztion - A How To Pictorial
http://www.shroomery.org/forums/showflat.php/Cat/0/Number/4527808/an/0/page/0

Make your own shroom chocolates
http://www.shroomery.org/forums/showflat.php/Cat/0/Number/4500682/an/0/page/5

guide with pix.


Make Your Own Magnetic Stirrer! How-To Tek w/ pix
http://www.shroomery.org/forums/showflat.php/Cat/0/Number/5865949/an/0/page/3


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OfflineDjango12
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Re: The Only LC Tech! The Liquid Culture How To Guide [Re: No_Life_G33k]
    #10577090 - 06/26/09 02:26 PM (14 years, 7 months ago)

Hate to resurrect this thread, and this might be a stupid question..But do you inject through the rubber stopper, and aspirate through the filter? or do you inject and aspirate through the rubber port? Thanks.


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InvisibleCH HELL
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Re: The Only LC Tech! The Liquid Culture How To Guide [Re: Django12]
    #10577103 - 06/26/09 02:29 PM (14 years, 7 months ago)

Never stick a needle through the fliter!  Every thing should be done through the injection port.
CH


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InvisibleAyrios
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Re: The Only LC Tech! The Liquid Culture How To Guide [Re: tahoe]
    #10754596 - 07/28/09 10:16 AM (14 years, 6 months ago)

I have a couple quart jars coming along very nicely at 9 days...  I'm just wondering when I'll be able to use ALL of it.  Currently, there are a couple dozen clusters of myc in each jar with a lot of tiny specks of myc.  In the past, I haven't needed to use LCs right away, but now I have a bunch of in vitro bags I want to noc up.  Even though there's only a bit of clustered myc in each jar, can I still agitate them, suck up 60 cc per bag, and noc them up?  Would it be better to wait till it's much thicker?

And I don't feel bad about resurrecting this thread as it's seemingly the most complete LC tek I've read :P


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Offlineveda_sticks
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Re: The Only LC Tech! The Liquid Culture How To Guide [Re: Ayrios]
    #10754620 - 07/28/09 10:20 AM (14 years, 6 months ago)

It would be better to wait until its ready.

In the meantime, inoc a few bags up with spores. It will serve as a backup incase the LC doesnt work out.


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek


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InvisibleAyrios
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Re: The Only LC Tech! The Liquid Culture How To Guide [Re: Ayrios]
    #10754663 - 07/28/09 10:33 AM (14 years, 6 months ago)

Actually I'm working with sterile substrate bags (MM-style), so I need lots of LC :P  I will just wait another week or so and see how it's looking then.


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Shop: Kraken Kratom Red Vein Kratom   Left Coast Kratom Buy Kratom Extract   North Spore Bulk Substrate   Mushroom-Hut Liquid Cultures


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