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gc1966
The Journeyman

Registered: 12/12/07
Posts: 177
Loc: Sydney, Australia
Last seen: 1 month, 13 days
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Ist Timer, I fucked up sterilization, I think
#7886097 - 01/15/08 09:36 PM (16 years, 17 days ago) |
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When inoculating my substrate, I did not wipe the back stuff off the needle after injecting,the carbon left from the needle seems to be located in the top layer of dry vermiculite, although I,m concerned it may impact the whole mycelium growing process.Also how long a period will it take before I start seeing evidence of mycelium growth?
-------------------- The strangest life I've ever known
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TobesCanaian


Registered: 12/05/07
Posts: 114
Loc:
Last seen: 3 years, 8 months
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Re: Ist Timer, I fucked up sterilization, I think [Re: gc1966]
#7886417 - 01/15/08 10:35 PM (16 years, 17 days ago) |
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There is no way you can fix that now so the only thing you can do is wait, I have had cakes that started going right away and some that took a couple weeks depends on the type of spore, and it doesnt really sound like you injected past the top vermiculite layer so it will take a little longer if it works at all
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bait_
AllSpice

Registered: 04/24/07
Posts: 2,292
Loc: This Cannot Be Happening
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Re: Ist Timer, I fucked up sterilization, I think [Re: TobesCanaian]
#7886435 - 01/15/08 10:38 PM (16 years, 17 days ago) |
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expect to see growth in about 3 days maybe 5. after that I tend to start to get worried.
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Beebo
Stranger



Registered: 01/17/06
Posts: 197
Last seen: 14 years, 4 months
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Re: Ist Timer, I fucked up sterilization, I think [Re: TobesCanaian]
#7886458 - 01/15/08 10:41 PM (16 years, 17 days ago) |
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Well, since you're talking about the top layer of dry vermiculite, I'm going to assume that you're talking about the PF-tek, yes? Well don' tworry about the carbon, I've put needles in red hot before and injected and they were fine. Though that's not a good idea what I used to do, so don't do that.
The time however depends on many factors, but I'd say if nothing shows up in two weeks, then you should get worried. It took me about..8 days to see growth, so don't really expect to see growth in 3 days. Usually if there is growth in three days, and by day 7 it looks completely overtaken, it's most likely not myc! Usually with this hobby, slow and steady wins the race. At-least, that's my take on it. Good luck growing
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The shroomy 1
Luminous beings surround me




Registered: 03/27/07
Posts: 5,543
Loc: The Aether
Last seen: 5 months, 5 days
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Re: Ist Timer, I fucked up sterilization, I think [Re: Beebo]
#7886605 - 01/15/08 11:17 PM (16 years, 17 days ago) |
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Carbon is not a problem, Don't sweat that. You should see something in about 5 days.
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