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Offlinenekrakys
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Adding B group vitamins to substrate[UPDATE 08.08] big flush
    #7881704 - 01/15/08 03:34 AM (16 years, 17 days ago)

Hi everyone, somewhere i read that B group vitamins required for synthesis of psilocin and psilocybin in shroom. So when none of B vitamins is presence, psilocin synthesis is stopped(when using white rice instead brown one) So i decided to add some vitamins to my pf substrate.
Inoculated 5 jars with PF strain. 4 jars was sterilized in pot for 45Min's and 1 in microwave under 350W for 2min. All jars are 5 days old, mycelium is growing and no contamination  :uptosomething:


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Edited by nekrakys (02/08/08 03:31 AM)


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Offlineveda_sticks
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Re: Adding B group vitamins to substrate [Re: nekrakys]
    #7881773 - 01/15/08 05:02 AM (16 years, 17 days ago)

There was a discussion about something similar to this not to long ago, have a search for it.

I cant remember of the top of my head, but im sure its not just vitimans, theres are other things to take into account.

Mycelium eats food.


--------------------

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Offlinenekrakys
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Re: Adding B group vitamins to substrate [Re: veda_sticks]
    #7882922 - 01/15/08 11:28 AM (16 years, 17 days ago)

Well ill keep informing. Hope that potency will enormous


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Invisiblepoot
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Re: Adding B group vitamins to substrate [Re: nekrakys]
    #7883588 - 01/15/08 02:03 PM (16 years, 17 days ago)

Quote:

nekrakys said:
Hope that potency will enormous [/quote

a potency is. an enormous i hope then yes. oh yes! people yes! 5 shrooms for you!


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Offlinenekrakys
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Re: Adding B group vitamins to substrate [Re: poot]
    #7883850 - 01/15/08 03:00 PM (16 years, 17 days ago)

Hope so :uptosomething:


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Invisiblebadman
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Re: Adding B group vitamins to substrate [Re: nekrakys]
    #7884741 - 01/15/08 05:36 PM (16 years, 17 days ago)

You may be on to a winner vitamin B complexes contain nitrogen and aromatic rings similar to psilocybin. Potency might be improved by adding the amino acids Phenylalanine and tryptophan.


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Offlineverminute
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Re: Adding B group vitamins to substrate [Re: badman]
    #7884777 - 01/15/08 05:45 PM (16 years, 17 days ago)

I was planning on a similar theory, except I was going to use Whey Protein which contains B vitamins and amino acids... Plus a lot of other shit that I don't understand...


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Invisiblefastfred
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Re: Adding B group vitamins to substrate [Re: nekrakys]
    #7886640 - 01/15/08 11:23 PM (16 years, 17 days ago)

Quote:

nekrakys said:
Hi everyone, somewhere i read that B group vitamins required for synthesis of psilocin and psilocybin in shroom. So when none of B vitamins is presence, psilocin synthesis is stopped




Actually I think that's just something you made up.

Perhaps adding some wishfull thinking and a touch of placebo effect will give you crazy potent shroomz! Better write up a tek!


-FF


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Offlinenekrakys
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Re: Adding B group vitamins to substrate [Re: fastfred]
    #7886997 - 01/16/08 01:20 AM (16 years, 16 days ago)

6th day of grow. Mycelium growing crazy, no contam so far (note that 1 jar was pre-sterilized in MW for 2mins!


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OfflineRogerRabbitM
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Re: Adding B group vitamins to substrate [Re: nekrakys]
    #7887584 - 01/16/08 08:56 AM (16 years, 16 days ago)

Quote:

4 jars was sterilized in pot for 45Min's and 1 in microwave under 350W for 2min. All jars are 5 days old, mycelium is growing and no contamination





Improper sterilization and 'mycelium going crazy' after six days? I hate to have to say it, but this sounds much more like mold mycelium than mushroom mycelium.
RR


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Offlinenekrakys
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Re: Adding B group vitamins to substrate [Re: RogerRabbit]
    #7888128 - 01/16/08 12:50 PM (16 years, 16 days ago)

yep, still no contams, not newbie in growing, so i know how mycelium looks like. Growth in MW sterilized is like slower that sterilized in pot. Spore syringe was prepared also not very sterile conditions. All contams i have is green mold, which dies at 40C maybe my room has only this contam, dont know.....

here the pics, the first from right is MW pre-sterilized



Maybe MW'ed one has slower grow, because nutrients had been nuked away :confused:
Note that i used mine spore syringe from 3 prints, so it makes difference i think from casual syringes


Edited by nekrakys (01/16/08 01:06 PM)


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Offlineverminute
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Re: Adding B group vitamins to substrate [Re: nekrakys]
    #7888138 - 01/16/08 12:52 PM (16 years, 16 days ago)

In the MW I assume you left the metal mason top off?


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Offlinenekrakys
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Re: Adding B group vitamins to substrate [Re: verminute]
    #7888159 - 01/16/08 12:57 PM (16 years, 16 days ago)

i left foil on top :rolleyes:


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Offlineverminute
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Re: Adding B group vitamins to substrate [Re: nekrakys]
    #7888982 - 01/16/08 04:31 PM (16 years, 16 days ago)

Did the MW blow up!? :smile:


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Offlineverminute
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Re: Adding B group vitamins to substrate [Re: nekrakys]
    #7889026 - 01/16/08 04:41 PM (16 years, 16 days ago)

Quote:

nekrakys said:
All contams i have is green mold, which dies at 40C maybe my room has only this contam, dont know.....

Maybe MW'ed one has slower grow, because nutrients had been nuked away :confused:
Note that i used mine spore syringe from 3 prints, so it makes difference i think from casual syringes




If you get green mold toss it out! 40oC will kill mycelium anyway, don't open, just toss, even if you have health insureance, not worth the risk buddy...

Heat from boiling or microwave is not enough to vaporize nutrients from what I believe, someone please correct me if I'm wrong.


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Offlinenekrakys
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Re: Adding B group vitamins to substrate [Re: verminute]
    #7891080 - 01/16/08 11:31 PM (16 years, 16 days ago)

i talk about green mold spores, not mycelium :cool: and.... green mold is not dangerous, its from penicilinum group :smile:


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InvisibleJewelessCaesar
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Re: Adding B group vitamins to substrate [Re: nekrakys]
    #7891188 - 01/16/08 11:53 PM (16 years, 16 days ago)

Quote:

nekrakys said:
i talk about green mold spores, not mycelium :cool: and.... green mold is not dangerous, its from penicilinum group :smile:




Contams



Read that.


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Offlinenekrakys
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Re: Adding B group vitamins to substrate [Re: JewelessCaesar]
    #7891371 - 01/17/08 12:31 AM (16 years, 16 days ago)

Well, in my studies i have cycle of microbiology, and i know that pennicilinum group only can harm if you're alergic. Course i dont advice   
doing such as things i do, its only experiment , :uptosomething:


PS. MW didnt blow, why it had? I always MW'ing my food in foil :rolleyes:


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Offlineverminute
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Re: Adding B group vitamins to substrate [Re: nekrakys]
    #7892856 - 01/17/08 12:39 PM (16 years, 15 days ago)

Penicillin was a break through just on mistake too I believe.

Do you see sparks or lightening bolts in the microwave with the foil? You must have a nice microwave he he :smile:


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Re: Adding B group vitamins to substrate [Re: verminute]
    #7893445 - 01/17/08 03:23 PM (16 years, 15 days ago)

Green mold must DIE:butcher:


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Re: Adding B group vitamins to substrate [Re: Blutjager]
    #7893473 - 01/17/08 03:30 PM (16 years, 15 days ago)

are you doing a side by side comparison of b vitamin enhanced substrate and normal substrate? I would do that and then administer a double blind test on your friends to determine potency.


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Offlinenekrakys
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Re: Adding B group vitamins to substrate [Re: Crasher]
    #7896178 - 01/18/08 06:30 AM (16 years, 14 days ago)

But mycelium growing rate is awesome comparing to my latest batch. Day 8 after inoculation. BTW i don't see any sparks on foil :crazy2: The left one in pic is MW'ed, possible water nuked out?



And yes, placebo effect can make difference when determinating potency, well still happy that adding vitamins dont ruined my batch yet.


----UPDATE----- 01.28 Well three cakes dunked,cold shocked,rolled in verm now sitting in 70liter tub on perlite at 21C. The MW'ed jar became stalled for long time, and get contaminated by Green HULK :sad:


The right one is recovered from Mr.Green mold (not enough verm on top of jar):spank: and now is waiting to revenge by pumping psilocybin in mushies 


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Edited by nekrakys (01/26/08 04:37 AM)


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Offlinenekrakys
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Re: Adding B group vitamins to substrate [Re: nekrakys]
    #7933731 - 01/26/08 04:43 AM (16 years, 6 days ago)

Mushies will be given away to Trip King to determing potency of these goodies. Trip King shouldnt know about B vitamins.....


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OfflineCiv
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Re: Adding B group vitamins to substrate [Re: nekrakys]
    #7933879 - 01/26/08 08:00 AM (16 years, 6 days ago)

*sits back and pulls out some popcorn*

Your grow area is disgustingly dirty. Your method to guess potency by feeding them to a friend isn't going to give you the level of psy/baeopsy in the batch or any information that could help you put a number on the strength.

You extract from two seperate batches, then compare results. Its about to be a contamination wonderland ... keep us updated.


Edited by Civ (01/26/08 08:08 AM)


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OfflineCiv
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Re: Adding B group vitamins to substrate [Re: fastfred]
    #7933898 - 01/26/08 08:11 AM (16 years, 6 days ago)

Quote:

Actually I think that's just something you made up.

Perhaps adding some wishfull thinking and a touch of placebo effect will give you crazy potent shroomz! Better write up a tek!





FF!  Omg I really needed that this morning :smile:


--------------------
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So, wash it well afterwards & DON'T tell them..."  -Agar


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Offlinenekrakys
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Re: Adding B group vitamins to substrate [Re: Civ]
    #7933908 - 01/26/08 08:14 AM (16 years, 6 days ago)

yep, poor conditions in prison, its called Dirty's TEK :crazy2:


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Re: Adding B group vitamins to substrate [Re: nekrakys]
    #7934012 - 01/26/08 08:47 AM (16 years, 6 days ago)

microwaving foil or any metal is dangerous.. are you trying to blow up you kitchen? there is no way you can have metal in there without it getting hot, sparking or lighting ablaze... I cannot believe that you have the only microwave in the history of the world the doesnt react when metal is set inside.. at least just boil your jars... it works great for pf tek jars... you for sure need a PC for grain, though...

again, unless you want to be another fire death statistic, I suggest NOT MICROWAVING ANYTHING METAL... mostley...


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Offlinenekrakys
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Re: Adding B group vitamins to substrate [Re: DJYoshaBYD]
    #7934022 - 01/26/08 08:52 AM (16 years, 6 days ago)

Yeah i bought PC  last week, today i inoculated 1 pint and 1 quart jar of rye.

But still, MW doesnt spark, i always MWing my chicken in foil... it doesnt blow... i thought it can be on the movies :confused:


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Offlineverminute
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Re: Adding B group vitamins to substrate [Re: nekrakys]
    #7934443 - 01/26/08 11:13 AM (16 years, 6 days ago)

Quote:

nekrakys said:
Yeah i bought PC  last week, today i inoculated 1 pint and 1 quart jar of rye.

But still, MW doesnt spark, i always MWing my chicken in foil... it doesnt blow... i thought it can be on the movies :confused:




If it works in the movies then for sure it will work in real life. We all know that...


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Offlinenekrakys
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Re: Adding B group vitamins to substrate [Re: verminute]
    #7934724 - 01/26/08 12:17 PM (16 years, 6 days ago)

not blowing for me, maybe it has protection or smth....


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Offlineverminute
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Re: Adding B group vitamins to substrate [Re: nekrakys]
    #7934964 - 01/26/08 01:01 PM (16 years, 6 days ago)

I'm sorry dude, I can't understand what you're saying... Is english your second language? Tu parle francias?


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Offlinenekrakys
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Re: Adding B group vitamins to substrate [Re: verminute]
    #7935218 - 01/26/08 01:49 PM (16 years, 6 days ago)

:spank: im saying: MW dont blow when nuking foil. is that clear? каков ваш второй язык? Испанский?


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Offlinenekrakys
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Re: Adding B group vitamins to substrate [Re: Blutjager]
    #7990508 - 02/07/08 04:35 AM (15 years, 11 months ago)

well three cakes fruiting pretty well.Lots of direct misting, PMP 24/7. As you can see there is still water around cakes, so myc can leach it. 146 pins by now :nicekitty:




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Edited by nekrakys (02/07/08 04:46 AM)


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Offlinenekrakys
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Re: Adding B group vitamins to substrate [Re: nekrakys]
    #7994802 - 02/08/08 02:52 AM (15 years, 11 months ago)

175g from first flush :ooo: Potency will be tested tonight :naughty:


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Edited by nekrakys (02/08/08 03:27 AM)


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OfflineCakes
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Re: Adding B group vitamins to substrate [Re: nekrakys]
    #7994876 - 02/08/08 04:19 AM (15 years, 11 months ago)

Not bad from 3 cakes :thumbup:


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OfflineNibin
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Re: Adding B group vitamins to substrate [Re: nekrakys]
    #7994977 - 02/08/08 06:07 AM (15 years, 11 months ago)

Quote:

nekrakys said:
Well, in my studies i have cycle of microbiology, and i know that pennicilinum group only can harm if you're alergic. Course i dont advice   
doing such as things i do, its only experiment , :uptosomething:


PS. MW didnt blow, why it had? I always MW'ing my food in foil :rolleyes:




First of all, any kind of spore in the lungs is quite bad for you. Granted there are much worse things to breathe in than penicillium mold spores but they aren't harmless.

Second. Unless you are working from a genetic isolate the whole experiment will be a load of bs as you won't have the same genetics in each jar and so won't be able to compare


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OfflineRogerRabbitM
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Re: Adding B group vitamins to substrate [Re: Nibin]
    #7994991 - 02/08/08 06:16 AM (15 years, 11 months ago)

I can't seem to get past the 'green mold is part of the penicillium group' part. :rolleyes:
RR


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Offlinenekrakys
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Re: Adding B group vitamins to substrate [Re: RogerRabbit]
    #7995090 - 02/08/08 07:32 AM (15 years, 11 months ago)

someone will :mushroom2:


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InvisibleHippie3
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Re: Adding B group vitamins to substrate [Re: nekrakys]
    #7995199 - 02/08/08 08:19 AM (15 years, 11 months ago)

Quote:

nekrakys said:
well three cakes fruiting pretty well.Lots of direct misting, PMP 24/7. As you can see there is still water around cakes, so myc can leach it. 146 pins by now :nicekitty:







can't argue with results like that.
not really PROOF of anything
but still a nice flush.
btw
some newer microwaves can adjust for foil
and not spark. mine is that way,
just don't try nuking a fork.


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Invisibleshroober
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Re: Adding B group vitamins to substrate [Re: Hippie3]
    #7995301 - 02/08/08 08:56 AM (15 years, 11 months ago)

those are looking pretty nice but of course since this is not a controlled experiment its impossible to tell if the B vitamins had any effect, nice grow though, congrats!


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Re: Adding B group vitamins to substrate [Re: shroober]
    #7995369 - 02/08/08 09:15 AM (15 years, 11 months ago)

Thanx, its more like grow log. :grin: Potency average, not what i have expected, 16g of fresh mushies consumed 6 hours ago, not so strong visuals, etc... Well, still happy thant flush doesnt fk'd'up :blowme:


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Re: Adding B group vitamins to substrate [Re: fastfred]
    #7995493 - 02/08/08 09:44 AM (15 years, 11 months ago)

Quote:

fastfred said:
Actually I think that's just something you made up.

Perhaps adding some wishfull thinking and a touch of placebo effect will give you crazy potent shroomz!  Better write up a tek!


-FF




People should do their homework and not waste threads :smile:  Search much?


--------------------
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So, wash it well afterwards & DON'T tell them..."  -Agar


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Invisibleshroober
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Re: Adding B group vitamins to substrate [Re: Civ]
    #7995554 - 02/08/08 09:57 AM (15 years, 11 months ago)

Quote:

Civ said:
People should do their homework and not waste threads :smile:  Search much?




Yeah, because we are quickly running out of threads and as we all know they are a very limited resource. Once we run out of threads I guess the shroomery will just be shut down, I think thats how the internet works.

In reality however, that was a dick-head statement, this person is discussing valid information and contributing quality pictures. The only person that "wasted a thread" was you.


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Re: Adding B group vitamins to substrate [Re: shroober]
    #7995569 - 02/08/08 09:59 AM (15 years, 11 months ago)

dude wtf

mircowaves cause electric arcs in most metals including aluminum and tin (aka foil)

i call bullshit.

http://video.aol.com/video-detail/aluminum-foil-im-microwave/689781671


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Re: Adding B group vitamins to substrate [Re: anarchOi]
    #7995653 - 02/08/08 10:17 AM (15 years, 11 months ago)

Mine doesnt spark, srry dude, PEACE ALL :rasta:
LOVE you guys :nicekitty:


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Edited by nekrakys (02/08/08 10:18 AM)


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Re: Adding B group vitamins to substrate [Re: nekrakys]
    #7996141 - 02/08/08 12:54 PM (15 years, 11 months ago)

Quote:

nekrakys said:
Well, in my studies i have cycle of microbiology, and i know that pennicilinum group only can harm if you're alergic. Course i dont advice   
doing such as things i do, its only experiment , :uptosomething:


PS. MW didnt blow, why it had? I always MW'ing my food in foil :rolleyes:



You have very nice cakes (fruits), but for me this thread is :thumbdown: bullshit; and i hope that noobs don't stumble across. IMHO!!!


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And on the 7. day the creator designed the psychedelic drugs. Holy shit. Thats intelligent design far beyond my scope. :wink:
Namaste


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OfflineanarchOi
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Re: Adding B group vitamins to substrate [Re: udok]
    #7999526 - 02/09/08 09:51 AM (15 years, 11 months ago)

perhaps you mean conventional oven instead of microwave?


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Re: Adding B group vitamins to substrate [Re: anarchOi]
    #7999766 - 02/09/08 11:36 AM (15 years, 11 months ago)

:spank: Its MW!!!!!!!! damnit


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OfflineZinglons Acolyte
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Re: Adding B group vitamins to substrate [Re: nekrakys]
    #7999835 - 02/09/08 11:56 AM (15 years, 11 months ago)

there are convection microwaves that function as a microwave AND an oven


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And they wandered off.. nine ways till bedfast.
-----
"And lets pray that there's intelligent life somewhere up in space 'cause theres bugger-all down here on earth!"  -Monty Python's "The Universe Song" from The Meaning of Life
"In the beginning, the universe was created. This has made a lot of people very angry and has been widely considered as a bad move."  -Douglas Adams "The Hitchhikers Guide to the Galaxy"
"If the words 'life, liberty and the pursuit of happiness' don't include the right to experiment with your own consciousness, then the Declaration of Independence isn't worth the hemp it was written on." -Terence McKenna


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Re: Adding B group vitamins to substrate [Re: Zinglons Acolyte]
    #8003516 - 02/10/08 02:28 AM (15 years, 11 months ago)

This thread is not bout  MWs :mad2: This thread is BS and person started it Total N00B :runaway:


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Re: Adding B group vitamins to substrate [Re: nekrakys]
    #8003549 - 02/10/08 02:51 AM (15 years, 11 months ago)

the fundamental problem with the concept of this is that it neglects the fact that mushrooms like to make b group vitamins especially (or so I read in a book on mushrooms). I bet they synthesize it from amino acids or cellulose or both. I don't really know but if you could find out it could be a significant improvement. The fact that Hpoo is considered one of the best substrates indicates it is likely both, since hpoo is not heavily digested and contains amino acids nitrogen and unprocessed cellulose. I saw a psillocin molecule on a different board before and it looked like it could be made from cellulose and nitrogen and little else though my memory is foggy. However whatever enzymes are used to do that also factor into the equation.

Well my brain hurts now. I can't think of anything else that could be construed as helpful to say.


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"Have you ever seen what happens when a grenade goes off in a school? Do you really know what you’re doing when you order shock and awe? Are you prepared to kneel beside a dying soldier and tell him why he went to Iraq, or why he went to any war?"
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Re: Adding B group vitamins to substrate [Re: ScavengerType]
    #8004924 - 02/10/08 02:37 PM (15 years, 11 months ago)

as a scientific experiment, this lacked proper controls, as well as proper means of testing and therefore the entire experiment is flawed, you need to do more research about mushies before doing things like this, cause this was pretty much a waste of effort and not very well thought out


--------------------
And they wandered off.. nine ways till bedfast.
-----
"And lets pray that there's intelligent life somewhere up in space 'cause theres bugger-all down here on earth!"  -Monty Python's "The Universe Song" from The Meaning of Life
"In the beginning, the universe was created. This has made a lot of people very angry and has been widely considered as a bad move."  -Douglas Adams "The Hitchhikers Guide to the Galaxy"
"If the words 'life, liberty and the pursuit of happiness' don't include the right to experiment with your own consciousness, then the Declaration of Independence isn't worth the hemp it was written on." -Terence McKenna


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Re: Adding B group vitamins to substrate [Re: Zinglons Acolyte]
    #8005000 - 02/10/08 03:03 PM (15 years, 11 months ago)

To be fair I think he was going to make cakes anyway it probibly wasn't much effort in the first place.


--------------------
"Have you ever seen what happens when a grenade goes off in a school? Do you really know what you’re doing when you order shock and awe? Are you prepared to kneel beside a dying soldier and tell him why he went to Iraq, or why he went to any war?"
"The things that are done in the name of the shareholder are, to me, as terrifying as the things that are done—dare I say it—in the name of God. Montesquieu said, "There have never been so many civil wars as in the Kingdom of God." And I begin to feel that’s true. The shareholder is the excuse for everything."
- Author and former M6/M5 agent John le Carré on Democracy Now.
Conquer's Club


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Re: Adding B group vitamins to substrate [Re: ScavengerType]
    #8007560 - 02/11/08 02:43 AM (15 years, 11 months ago)

Yep and 100g of fresh shrooms shrank into 6g crack-dry :rolleyes: to much direct misting... :sad:


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