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Offlinenekrakys
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Adding B group vitamins to substrate[UPDATE 08.08] big flush
    #7881704 - 01/15/08 03:34 AM (16 years, 17 days ago)

Hi everyone, somewhere i read that B group vitamins required for synthesis of psilocin and psilocybin in shroom. So when none of B vitamins is presence, psilocin synthesis is stopped(when using white rice instead brown one) So i decided to add some vitamins to my pf substrate.
Inoculated 5 jars with PF strain. 4 jars was sterilized in pot for 45Min's and 1 in microwave under 350W for 2min. All jars are 5 days old, mycelium is growing and no contamination  :uptosomething:


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Edited by nekrakys (02/08/08 03:31 AM)


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Offlineveda_sticks
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Re: Adding B group vitamins to substrate [Re: nekrakys]
    #7881773 - 01/15/08 05:02 AM (16 years, 17 days ago)

There was a discussion about something similar to this not to long ago, have a search for it.

I cant remember of the top of my head, but im sure its not just vitimans, theres are other things to take into account.

Mycelium eats food.


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Offlinenekrakys
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Re: Adding B group vitamins to substrate [Re: veda_sticks]
    #7882922 - 01/15/08 11:28 AM (16 years, 17 days ago)

Well ill keep informing. Hope that potency will enormous


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Invisiblepoot
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Re: Adding B group vitamins to substrate [Re: nekrakys]
    #7883588 - 01/15/08 02:03 PM (16 years, 17 days ago)

Quote:

nekrakys said:
Hope that potency will enormous [/quote

a potency is. an enormous i hope then yes. oh yes! people yes! 5 shrooms for you!


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Offlinenekrakys
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Re: Adding B group vitamins to substrate [Re: poot]
    #7883850 - 01/15/08 03:00 PM (16 years, 17 days ago)

Hope so :uptosomething:


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Invisiblebadman
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Re: Adding B group vitamins to substrate [Re: nekrakys]
    #7884741 - 01/15/08 05:36 PM (16 years, 17 days ago)

You may be on to a winner vitamin B complexes contain nitrogen and aromatic rings similar to psilocybin. Potency might be improved by adding the amino acids Phenylalanine and tryptophan.


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Offlineverminute
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Re: Adding B group vitamins to substrate [Re: badman]
    #7884777 - 01/15/08 05:45 PM (16 years, 17 days ago)

I was planning on a similar theory, except I was going to use Whey Protein which contains B vitamins and amino acids... Plus a lot of other shit that I don't understand...


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Invisiblefastfred
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Re: Adding B group vitamins to substrate [Re: nekrakys]
    #7886640 - 01/15/08 11:23 PM (16 years, 17 days ago)

Quote:

nekrakys said:
Hi everyone, somewhere i read that B group vitamins required for synthesis of psilocin and psilocybin in shroom. So when none of B vitamins is presence, psilocin synthesis is stopped




Actually I think that's just something you made up.

Perhaps adding some wishfull thinking and a touch of placebo effect will give you crazy potent shroomz! Better write up a tek!


-FF


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Offlinenekrakys
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Re: Adding B group vitamins to substrate [Re: fastfred]
    #7886997 - 01/16/08 01:20 AM (16 years, 17 days ago)

6th day of grow. Mycelium growing crazy, no contam so far (note that 1 jar was pre-sterilized in MW for 2mins!


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OfflineRogerRabbitM
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Re: Adding B group vitamins to substrate [Re: nekrakys]
    #7887584 - 01/16/08 08:56 AM (16 years, 16 days ago)

Quote:

4 jars was sterilized in pot for 45Min's and 1 in microwave under 350W for 2min. All jars are 5 days old, mycelium is growing and no contamination





Improper sterilization and 'mycelium going crazy' after six days? I hate to have to say it, but this sounds much more like mold mycelium than mushroom mycelium.
RR


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Offlinenekrakys
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Re: Adding B group vitamins to substrate [Re: RogerRabbit]
    #7888128 - 01/16/08 12:50 PM (16 years, 16 days ago)

yep, still no contams, not newbie in growing, so i know how mycelium looks like. Growth in MW sterilized is like slower that sterilized in pot. Spore syringe was prepared also not very sterile conditions. All contams i have is green mold, which dies at 40C maybe my room has only this contam, dont know.....

here the pics, the first from right is MW pre-sterilized



Maybe MW'ed one has slower grow, because nutrients had been nuked away :confused:
Note that i used mine spore syringe from 3 prints, so it makes difference i think from casual syringes


Edited by nekrakys (01/16/08 01:06 PM)


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Offlineverminute
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Re: Adding B group vitamins to substrate [Re: nekrakys]
    #7888138 - 01/16/08 12:52 PM (16 years, 16 days ago)

In the MW I assume you left the metal mason top off?


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Offlinenekrakys
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Re: Adding B group vitamins to substrate [Re: verminute]
    #7888159 - 01/16/08 12:57 PM (16 years, 16 days ago)

i left foil on top :rolleyes:


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Offlineverminute
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Re: Adding B group vitamins to substrate [Re: nekrakys]
    #7888982 - 01/16/08 04:31 PM (16 years, 16 days ago)

Did the MW blow up!? :smile:


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Offlineverminute
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Re: Adding B group vitamins to substrate [Re: nekrakys]
    #7889026 - 01/16/08 04:41 PM (16 years, 16 days ago)

Quote:

nekrakys said:
All contams i have is green mold, which dies at 40C maybe my room has only this contam, dont know.....

Maybe MW'ed one has slower grow, because nutrients had been nuked away :confused:
Note that i used mine spore syringe from 3 prints, so it makes difference i think from casual syringes




If you get green mold toss it out! 40oC will kill mycelium anyway, don't open, just toss, even if you have health insureance, not worth the risk buddy...

Heat from boiling or microwave is not enough to vaporize nutrients from what I believe, someone please correct me if I'm wrong.


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Offlinenekrakys
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Re: Adding B group vitamins to substrate [Re: verminute]
    #7891080 - 01/16/08 11:31 PM (16 years, 16 days ago)

i talk about green mold spores, not mycelium :cool: and.... green mold is not dangerous, its from penicilinum group :smile:


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InvisibleJewelessCaesar
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Re: Adding B group vitamins to substrate [Re: nekrakys]
    #7891188 - 01/16/08 11:53 PM (16 years, 16 days ago)

Quote:

nekrakys said:
i talk about green mold spores, not mycelium :cool: and.... green mold is not dangerous, its from penicilinum group :smile:




Contams



Read that.


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Offlinenekrakys
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Re: Adding B group vitamins to substrate [Re: JewelessCaesar]
    #7891371 - 01/17/08 12:31 AM (16 years, 16 days ago)

Well, in my studies i have cycle of microbiology, and i know that pennicilinum group only can harm if you're alergic. Course i dont advice   
doing such as things i do, its only experiment , :uptosomething:


PS. MW didnt blow, why it had? I always MW'ing my food in foil :rolleyes:


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Offlineverminute
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Re: Adding B group vitamins to substrate [Re: nekrakys]
    #7892856 - 01/17/08 12:39 PM (16 years, 15 days ago)

Penicillin was a break through just on mistake too I believe.

Do you see sparks or lightening bolts in the microwave with the foil? You must have a nice microwave he he :smile:


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InvisibleBlutjager
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Re: Adding B group vitamins to substrate [Re: verminute]
    #7893445 - 01/17/08 03:23 PM (16 years, 15 days ago)

Green mold must DIE:butcher:


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