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Offlinemushsage
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Registered: 10/27/07
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Last seen: 15 years, 10 months
what generation am i on?
    #7879641 - 01/14/08 06:22 PM (16 years, 18 days ago)

okay i'm kinda confused as to which generation i'm on; here's what i've done...

multispore > grain jar > straw > harvested mushroom tissue to petri which i will then > grain jar > straw

what generation am i at now (with my petri) and what generation will at be at the end?

and will going from my current petri to another petri add another generation? (i read that having to do few cell divisions to colonize a petri that it's not such as problem as having to colonize an entire jar)


Edited by mushsage (01/14/08 11:48 PM)


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Offlineravin0fff
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Registered: 10/17/07
Posts: 228
Last seen: 15 years, 8 months
Re: Reusing frozen agar/steralizing glass petris/what generation am i on? [Re: mushsage]
    #7879985 - 01/14/08 07:20 PM (16 years, 18 days ago)

i'm looking to do some reishi cultures, so i'de like to know some of this stuff as well


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Offlineravin0fff
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Registered: 10/17/07
Posts: 228
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Re: Reusing frozen agar/steralizing glass petris/what generation am i on? [Re: ravin0fff]
    #7880670 - 01/14/08 10:01 PM (16 years, 18 days ago)

bump


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Offlinemushsage
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Registered: 10/27/07
Posts: 22
Last seen: 15 years, 10 months
Re: Reusing frozen agar/steralizing glass petris/what generation am i on? [Re: ravin0fff]
    #7881188 - 01/14/08 11:49 PM (16 years, 18 days ago)

bump


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InvisibleCrasher
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Re: Reusing frozen agar/steralizing glass petris/what generation am i on? [Re: mushsage]
    #7881204 - 01/14/08 11:53 PM (16 years, 18 days ago)

don't bump.


--------------------
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Give me struggle, iron, volcanoes...


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OfflineThe shroomy 1
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Re: Reusing frozen agar/steralizing glass petris/what generation am i on? [Re: Crasher]
    #7881313 - 01/15/08 12:23 AM (16 years, 18 days ago)

Hell, you're "cloning" the original fruit body.


Edited by The shroomy 1 (01/15/08 12:27 AM)


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Invisiblepoot
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Registered: 11/24/07
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Re: Reusing frozen agar/steralizing glass petris/what generation am i on? [Re: The shroomy 1]
    #7881382 - 01/15/08 12:48 AM (16 years, 18 days ago)

bump


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Offlineveda_sticks
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Re: Reusing frozen agar/steralizing glass petris/what generation am i on? [Re: poot]
    #7881779 - 01/15/08 05:07 AM (16 years, 17 days ago)

I would hazard a guess at being 2nd generation.

Please read the forum rules, wait a day before bumping a post.


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Invisibletahoe
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Re: Reusing frozen agar/steralizing glass petris/what generation am i on? [Re: veda_sticks]
    #7882746 - 01/15/08 11:04 AM (16 years, 17 days ago)

why do you care what generation you are on?
the spore syringe is probably a hundred generataions away from the original specimen.
Are you worried about getting a degenerate strain. it doesnt happen near as often as people make it seem.
If you are starting from a multispore than you will be fine.
Cloning a mushroom from a multi spore will not continue the generations.
I have heard that cloning clones might degreade them but i doupt you will ever get this far into cultivation.

Clone you best mushroom to agar. Split that petri up to 15 more petris. Work wiht one petri and store the rest in the fridge. If you are using agar to start grain masters then you will be fine.
You will be able to start 5-10 grain masters from each petri.

I see that I have a new mission in life.
1. keep your casings wet
2.lc's less sugar is better
3. dont big deal the degenerate strain thing


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


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Invisiblepoot
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Re: Reusing frozen agar/steralizing glass petris/what generation am i on? [Re: tahoe]
    #7883606 - 01/15/08 02:09 PM (16 years, 17 days ago)

damn thats an ass...


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