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mushsage
Stranger
Registered: 10/27/07
Posts: 22
Last seen: 15 years, 10 months
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what generation am i on?
#7879641 - 01/14/08 06:22 PM (16 years, 18 days ago) |
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okay i'm kinda confused as to which generation i'm on; here's what i've done...
multispore > grain jar > straw > harvested mushroom tissue to petri which i will then > grain jar > straw
what generation am i at now (with my petri) and what generation will at be at the end?
and will going from my current petri to another petri add another generation? (i read that having to do few cell divisions to colonize a petri that it's not such as problem as having to colonize an entire jar)
Edited by mushsage (01/14/08 11:48 PM)
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ravin0fff
Stranger

Registered: 10/17/07
Posts: 228
Last seen: 15 years, 8 months
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Re: Reusing frozen agar/steralizing glass petris/what generation am i on? [Re: mushsage]
#7879985 - 01/14/08 07:20 PM (16 years, 18 days ago) |
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i'm looking to do some reishi cultures, so i'de like to know some of this stuff as well
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ravin0fff
Stranger

Registered: 10/17/07
Posts: 228
Last seen: 15 years, 8 months
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Re: Reusing frozen agar/steralizing glass petris/what generation am i on? [Re: ravin0fff]
#7880670 - 01/14/08 10:01 PM (16 years, 18 days ago) |
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bump
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mushsage
Stranger
Registered: 10/27/07
Posts: 22
Last seen: 15 years, 10 months
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Re: Reusing frozen agar/steralizing glass petris/what generation am i on? [Re: ravin0fff]
#7881188 - 01/14/08 11:49 PM (16 years, 18 days ago) |
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bump
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Crasher
αἱρετίζω




Registered: 03/13/01
Posts: 6,220
Loc: Tardy to the Party
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Re: Reusing frozen agar/steralizing glass petris/what generation am i on? [Re: mushsage]
#7881204 - 01/14/08 11:53 PM (16 years, 18 days ago) |
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don't bump.
-------------------- Give me silence, water, hope; Give me struggle, iron, volcanoes...
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The shroomy 1
Luminous beings surround me




Registered: 03/27/07
Posts: 5,543
Loc: The Aether
Last seen: 5 months, 5 days
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Re: Reusing frozen agar/steralizing glass petris/what generation am i on? [Re: Crasher]
#7881313 - 01/15/08 12:23 AM (16 years, 18 days ago) |
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Hell, you're "cloning" the original fruit body.
Edited by The shroomy 1 (01/15/08 12:27 AM)
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poot
bottom feeder



Registered: 11/24/07
Posts: 212
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Re: Reusing frozen agar/steralizing glass petris/what generation am i on? [Re: The shroomy 1]
#7881382 - 01/15/08 12:48 AM (16 years, 18 days ago) |
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bump
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veda_sticks
Cultivator




Registered: 07/29/07
Posts: 14,191
Loc: UK
Last seen: 4 years, 25 days
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Re: Reusing frozen agar/steralizing glass petris/what generation am i on? [Re: poot]
#7881779 - 01/15/08 05:07 AM (16 years, 17 days ago) |
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I would hazard a guess at being 2nd generation.
Please read the forum rules, wait a day before bumping a post.
-------------------- PF TEK - writeup by EvilMushroom666 Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD Cakes can and will pin! - So you think cakes suck for pins. Your wrong Franks Simple Coir/Verm Tek Franks Proper Pasturisation Tek Franks Spawning To Bulk - Monotub Professor Pinheads RTV Injection Port Tek Foo Mans No Soak WBS Prep Tek
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tahoe
Noob Slayer



Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
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Re: Reusing frozen agar/steralizing glass petris/what generation am i on? [Re: veda_sticks]
#7882746 - 01/15/08 11:04 AM (16 years, 17 days ago) |
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why do you care what generation you are on? the spore syringe is probably a hundred generataions away from the original specimen. Are you worried about getting a degenerate strain. it doesnt happen near as often as people make it seem. If you are starting from a multispore than you will be fine. Cloning a mushroom from a multi spore will not continue the generations. I have heard that cloning clones might degreade them but i doupt you will ever get this far into cultivation.
Clone you best mushroom to agar. Split that petri up to 15 more petris. Work wiht one petri and store the rest in the fridge. If you are using agar to start grain masters then you will be fine. You will be able to start 5-10 grain masters from each petri.
I see that I have a new mission in life. 1. keep your casings wet 2.lc's less sugar is better 3. dont big deal the degenerate strain thing
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
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poot
bottom feeder



Registered: 11/24/07
Posts: 212
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Re: Reusing frozen agar/steralizing glass petris/what generation am i on? [Re: tahoe]
#7883606 - 01/15/08 02:09 PM (16 years, 17 days ago) |
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damn thats an ass...
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