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badman


Registered: 06/14/06
Posts: 4,039
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Two years of work...
#7877789 - 01/14/08 11:10 AM (16 years, 18 days ago) |
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During these two(ish) years, using PF cakes which are 1/5th of a pint i think i have created a B+ sub-strain which is VERY aggressive. By aggressive i mean it fights contamination. Has any one else got a contam resistant strain? I did this from spores then 20 sets of 10 clone (2 each) then using 15 generations of LCs.
One 1/2 pint will fully colonise in under two weeks when using LC PCed at 10psi for 15 maybe 20 mins excluding cool down time. using the same LC an unsterile cake will colonize in 4 weeks after fighting off contams.
Need some input from the community any ideas in cutting out the PCing and reducing colonistion time
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MYSTIQUE
Say Hi to the elves for me.




Registered: 04/28/07
Posts: 1,764
Loc: Canada
Last seen: 5 years, 4 months
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Re: Two years of work... [Re: badman]
#7878593 - 01/14/08 02:29 PM (16 years, 18 days ago) |
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Thats amazing how do I get a print.
-------------------- Dont know what the fuck I just said? READ THIS http://www.shroomery.org/5122/The-Shroomery-Mushroom-Glossary I ain't a hippy but I'm covered in dirt Sippin lots of mushroom tea in a tye-dye shirt Chasin' the Grateful Dead, no shoes on my feet Beggin' in the parking lot for something to eat, DO NOT USE FIRE IN YOUR GLOVE BOX!!!!!!!
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Cakes
some guy




Registered: 08/26/05
Posts: 1,613
Loc: Arizonee
Last seen: 7 months, 8 hours
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Re: Two years of work... [Re: MYSTIQUE]
#7878656 - 01/14/08 02:51 PM (16 years, 18 days ago) |
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Me too! I would totally pay for a print.
--------------------
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badman


Registered: 06/14/06
Posts: 4,039
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Re: Two years of work... [Re: Cakes]
#7878921 - 01/14/08 03:55 PM (16 years, 18 days ago) |
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yeh sure but not yet cos it hasn't been perfected once i can get them to colonize unsterile cakes quicker and with less time. the only thing is that B+ give big shrooms right, well these only give shrooms 4-5 inch MAX no matter how deep. once this is done im gonna try to increase the potency.
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badman


Registered: 06/14/06
Posts: 4,039
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Re: Two years of work... [Re: badman]
#7878965 - 01/14/08 04:03 PM (16 years, 18 days ago) |
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By the way ill only send prints to UK may mainland europe if im in good mood. USA laws seem a pretentious on the whole drugs front sorry
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MYSTIQUE
Say Hi to the elves for me.




Registered: 04/28/07
Posts: 1,764
Loc: Canada
Last seen: 5 years, 4 months
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Re: Two years of work... [Re: badman]
#7878996 - 01/14/08 04:08 PM (16 years, 18 days ago) |
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im from Canada lol
-------------------- Dont know what the fuck I just said? READ THIS http://www.shroomery.org/5122/The-Shroomery-Mushroom-Glossary I ain't a hippy but I'm covered in dirt Sippin lots of mushroom tea in a tye-dye shirt Chasin' the Grateful Dead, no shoes on my feet Beggin' in the parking lot for something to eat, DO NOT USE FIRE IN YOUR GLOVE BOX!!!!!!!
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fastfred
Old Hand



Registered: 05/17/04
Posts: 6,899
Loc: Dark side of the moon
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Re: Two years of work... [Re: MYSTIQUE]
#7879033 - 01/14/08 04:16 PM (16 years, 18 days ago) |
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What are you going to call the strain? Any side-by-side or controlled experiments yet?
Orissa India is claimed to be contaminant resistant. If you're looking for size and contam resistance that might be a good strain to incorporate into your line.
Also how would you propose to select for potency? With the number of samples you'd have to test I don't think bioassay is going to cut it.
-FF
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badman


Registered: 06/14/06
Posts: 4,039
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Re: Two years of work... [Re: fastfred]
#7879162 - 01/14/08 04:46 PM (16 years, 18 days ago) |
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fastfred thanks for the rating.
orissa im gonna have to aquire this, i have only been working with b+. i have done controls of course otherwise i couldn't say they had contam resistance right.
for potency assay (bit long winded) i have been drying them to 0% H20 and using mass from crude product as an indicator
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badman


Registered: 06/14/06
Posts: 4,039
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Re: Two years of work... [Re: badman]
#7879184 - 01/14/08 04:49 PM (16 years, 18 days ago) |
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you have some given me BAD ASS ideas fastfred
   yes mate IOU 2
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PoisonedV
Fuming Shrooming




Registered: 09/13/07
Posts: 398
Loc: Hell
Last seen: 9 years, 1 month
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Re: Two years of work... [Re: badman]
#7879566 - 01/14/08 06:07 PM (16 years, 18 days ago) |
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LOL, THE US IS PRETENTIOUS? uk drug laws are just as bad as the us also thats a stupid reason to deny prints
-------------------- Lazy...
Edited by PoisonedV (01/14/08 06:08 PM)
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Cakes
some guy




Registered: 08/26/05
Posts: 1,613
Loc: Arizonee
Last seen: 7 months, 8 hours
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Re: Two years of work... [Re: PoisonedV]
#7879582 - 01/14/08 06:11 PM (16 years, 18 days ago) |
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Prints are perfectly legal in almost all the US states.
--------------------
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pazzy
Always Hardcore


Registered: 08/24/07
Posts: 1,360
Last seen: 13 years, 2 months
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Re: Two years of work... [Re: Cakes]
#7879738 - 01/14/08 06:38 PM (16 years, 18 days ago) |
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Do the unsterilized cakes actually fruit or have you just checked if they manage to colonize?
Also I could be mistaken but I believe prints from your contam fighting specimen would not show the same trait.
-------------------- Are you happy for a miracle?
Absolutely no source checks/discussions.
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badman


Registered: 06/14/06
Posts: 4,039
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Re: Two years of work... [Re: pazzy]
#7882939 - 01/15/08 11:32 AM (16 years, 17 days ago) |
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Ok US laws are more pretentious than UKs. (maybe im being a bit harsh when its done ill consider it if you send me a self addressed envelope)
yeh they fruit 7-12 days in a FC.
pazzy 'contam fighting specimen would not show the same trait'
they still VERY heavy spore depositors and the caps are same colours as my standard B+s. the cakes are mostly hyphae loads of thread like structures which branch its quite annoying that shrooms are smaller in comparison to my standard B+s but im still developing it now that i have new ideas. i need to acquire orissa spores and in the summer when i get 2 months free from uni ill try to perfect it
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bait_
AllSpice

Registered: 04/24/07
Posts: 2,292
Loc: This Cannot Be Happening
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Re: Two years of work... [Re: badman]
#7883214 - 01/15/08 12:27 PM (16 years, 17 days ago) |
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You developed a stronger strain using only PF cakes? I would at least assume that you would have used some agar. 1/5 pint cakes seems really small. 1/2 pints are much more commonly used.
Also you said that this strain only becomes 4-5 tall no matter what depth of substrate they are grown on; yet you said your grows are on PF tek type cakes which would all have the same "depth" of substrate.
Just saying.
Edited by bait_ (01/15/08 12:33 PM)
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RogerRabbit
Bans for Pleasure


Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 3 days
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Re: Two years of work... (moved) [Re: badman]
#7883390 - 01/15/08 01:06 PM (16 years, 17 days ago) |
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This thread was moved from Advanced Mycology.
Reason: It needs to be pointed out that 'strains' don't contaminate, substrates do. However, it's entirely possible to inoculate an unsterilized pf cake with enough liquid/mycelium inoculant that the mushroom mycelium colonizes the cakes before contaminant molds, which must germinate from spores, can get a foothold.
By Advanced Forum guidelines, this belongs in general cultivation. I'll move it over there for you. Good luck with your experiments! RR
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badman


Registered: 06/14/06
Posts: 4,039
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Re: Two years of work... (moved) [Re: RogerRabbit]
#7884325 - 01/15/08 04:20 PM (16 years, 17 days ago) |
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only on PF cake yes since it is the easiest method, reason being i never ever get contams on agar (or WBS), never, even on home made stuff(potato). 1/5 cakes for rapid colonisation due to high SA:Vol ratio.
ive had straw at 5 inches even fruiting has occurred but no fruits as big as B+ standards(max iv had 9 maybe 10 inch) which will be consumed by contams.
Im thinking of using agar to breed various bacterium then introducing them to mycellium on agar. but im caught in two minds about this since im getting promising results with the methods we are employing at the moment.
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fastfred
Old Hand



Registered: 05/17/04
Posts: 6,899
Loc: Dark side of the moon
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Re: Two years of work... (moved) [Re: badman]
#7886204 - 01/15/08 09:55 PM (16 years, 17 days ago) |
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Don't let RR get you down badman. He likes to make up rules as he goes and characterize and categorize the thread as he sees fit.
He doesn't like to have more than 3-4 active threads in advanced any given week. Your thread will be buried three pages down after about an hour in cult, but I'll try to follow it at least.
To those of you reading this in cult: Let this serve as a warning against posting in advanced.
If you're going to post in advanced it better damn well be advanced... but not too advanced, any posts involving the use of robotics in mycology will be booted also.
-FF
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Crasher
αἱρετίζω




Registered: 03/13/01
Posts: 6,220
Loc: Tardy to the Party
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Re: Two years of work... (moved) [Re: fastfred]
#7886240 - 01/15/08 10:01 PM (16 years, 17 days ago) |
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bitter much?
-------------------- Give me silence, water, hope; Give me struggle, iron, volcanoes...
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flavoraid
now with twicethe ketamine andopiates!


Registered: 12/05/07
Posts: 1,678
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Re: Two years of work... [Re: MYSTIQUE]
#7886622 - 01/15/08 11:19 PM (16 years, 17 days ago) |
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Quote:
MYSTIQUE said: Thats amazing how do I get a print.
-------------------- coda said: imachavel, Man you really need to do some reading, the amount of bullshit you put into almost every single one of your posts is absolutely astounding.
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