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Invisiblebmiles
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My first grow.
    #7870639 - 01/12/08 07:56 PM (16 years, 20 days ago)

ok... so i got my spore syringe in the mail today, and decided i would get the rest of the supplies that i didn't have, so me and my girl drove all around to stores looking for the stuff. i think i got it all, if so i'm going to start the process tonight or tomorrow. i'm going to be using the pf tek method following the lets grow mushrooms dvd, and i also read the psilocybin mushroom handbook.

supplies:
b+


3.5 cubic feet bag of verm (couldn't find a small bag anywhere)


12 8ounce jars


glove box and pot for sterilizing


micropore tape and gloves


brown rice flour


i have a few questions for you guys...
brown rice flour is a bitch to find, so we ended up going to a chinese market and found rice bran, will this work?

when sterilzing, do you keep the micro pore tape on the jars? the jars will have foil on top of course.

also, do the other supplies look adequate? i'm sure i'll have more questions later on.


--------------------
Never go with a hippy to a second location.


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OfflineJustice_Fish
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Re: My first grow. [Re: bmiles]
    #7870649 - 01/12/08 07:58 PM (16 years, 20 days ago)

What's with the tape?
And the gloves?


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InvisibleCrasher
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Re: My first grow. [Re: Justice_Fish]
    #7870660 - 01/12/08 08:01 PM (16 years, 20 days ago)

The gloves are for the...glovebox.

Tape for covering inoculation holes.

Right?


--------------------
Give me silence, water, hope;
Give me struggle, iron, volcanoes...


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Invisiblebmiles
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Re: My first grow. [Re: Crasher]
    #7870665 - 01/12/08 08:01 PM (16 years, 20 days ago)

Quote:

Crasher said:
The gloves are for the...glovebox.

Tape for covering inoculation holes.

Right?




you are correct.


--------------------
Never go with a hippy to a second location.


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Invisiblebmiles
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Re: My first grow. [Re: bmiles]
    #7870689 - 01/12/08 08:07 PM (16 years, 20 days ago)

also, should i wash the jars before loading up the mycelium food?
and i found the answer to my question, rice bran does work instead of rice flour.


Edited by bmiles (01/12/08 08:22 PM)


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OfflineSportinStyle
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Re: My first grow. [Re: bmiles]
    #7870761 - 01/12/08 08:25 PM (16 years, 20 days ago)

Honestly the glovebox is not really needed. Sterlization is EXTREMELY important, but a glovebox for PFTek is almost overkill.

You would get by just the same by using a non airated area (no breezes) and by spraying lysol on the area you plan on using. Just pressur cook for the right amount of time, and make sure substrate recieves zero outside contact after that. I have never used a glove box, and my rye grow and PFTek both worked or are working fine, without contams.


--------------------
RIP Heath Ledger


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Invisiblebmiles
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Re: My first grow. [Re: SportinStyle]
    #7870784 - 01/12/08 08:30 PM (16 years, 20 days ago)

alright, i guess i'll use the plastic tote for my fruiting chamber then.
i don't have a pressure cooker, but i have a big enough pot to do 6 jars at a time, i'm going to steam sterilize them like they do in the dvd.


--------------------
Never go with a hippy to a second location.


Edited by bmiles (01/12/08 08:33 PM)


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OfflineCubie
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Re: My first grow. [Re: SportinStyle]
    #7870793 - 01/12/08 08:32 PM (16 years, 20 days ago)

Id recommend a pressure cooker over the pot if you have one handy. If not their expencive but good luck on your first grow make sure you don't skip even the simplest step and you'll do fine.


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Invisiblebmiles
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Re: My first grow. [Re: Cubie]
    #7870811 - 01/12/08 08:35 PM (16 years, 20 days ago)

buying all this stuff left me with no more money, so i'm stuck with the pot for now, but i think it should be fine. thanks for the advice.


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Never go with a hippy to a second location.


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OfflineCubie
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Re: My first grow. [Re: bmiles]
    #7870823 - 01/12/08 08:40 PM (16 years, 20 days ago)

Believe me I understand I was once in the same position


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Invisiblebmiles
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Re: My first grow. [Re: Cubie]
    #7870825 - 01/12/08 08:40 PM (16 years, 20 days ago)

did you steam sterilize without a PC?


--------------------
Never go with a hippy to a second location.


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OfflineSportinStyle
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Re: My first grow. [Re: bmiles]
    #7870833 - 01/12/08 08:43 PM (16 years, 20 days ago)

If using a pot, put it on medium setting and put on a lid. I boil for about 1 hour and 30 mins, pc for about 1 and 15.

Just like the other guy said, follow the directions to a "t" and you will be golden.

Good luck!


--------------------
RIP Heath Ledger


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Invisibledumbfounded1600
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Re: My first grow. [Re: bmiles]
    #7870856 - 01/12/08 08:48 PM (16 years, 20 days ago)

SportinStyle - Honestly the glovebox is not really needed. Sterlization is EXTREMELY important, but a glovebox for PFTek is almost overkill.

You would get by just the same by using a non airated area (no breezes) and by spraying lysol on the area you plan on using. Just pressur cook for the right amount of time, and make sure substrate recieves zero outside contact after that. I have never used a glove box, and my rye grow and PFTek both worked or are working fine, without contams.


1. You can never be too sterile. Gloves are better then none at which even though you wash your hands bacteria can still surface.
2. Lysol is just compressed ISO and overpriced..just use ISO in a empty spray bottle...works great. :smirk:
3. And make sure your substrate receives zero outside contact after that? It needs Gas Exchange or it'll stall.


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OfflineCubie
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Re: My first grow. [Re: SportinStyle]
    #7870857 - 01/12/08 08:48 PM (16 years, 20 days ago)

I used singirls zip lock tek.


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Offlinefstrainpain
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Re: My first grow. [Re: Cubie]
    #7870925 - 01/12/08 09:02 PM (16 years, 20 days ago)

i would have done without the rest and got a pc. i use regular brown rice grains and put 1/2 a cup in a blender for about 3 minutes. i don't use a glove box or anything just swabs needle with alcohol and flame sterilize between jars i pc em for 60 min and let cool over night. never had a jar get contaminated.


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Invisiblebmiles
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Re: My first grow. [Re: fstrainpain]
    #7871168 - 01/12/08 09:59 PM (16 years, 20 days ago)

i filled up the jars, time to sterilize them in the pot.


--------------------
Never go with a hippy to a second location.


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Invisiblebmiles
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Re: My first grow. [Re: bmiles]
    #7872143 - 01/13/08 01:51 AM (16 years, 19 days ago)

i had twelve jars, but it was my first time using a syringe and also my first time doing this, so i put too much juice into a few of them, so i only inoculated 6 jars. hopefully i'll have better odds anyway, with more spores in each jar, but now all i can do is wait. thanks for the help guys.
-bmiles


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Never go with a hippy to a second location.


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OfflinePoisonedV
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Re: My first grow. [Re: bmiles]
    #7872150 - 01/13/08 01:54 AM (16 years, 19 days ago)

Holy shit dude thats overkill. Ive only done a few, and hardly any sterilization, but they all made it


--------------------
Lazy...


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OfflineSkeeblix
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Re: My first grow. [Re: PoisonedV]
    #7872163 - 01/13/08 01:59 AM (16 years, 19 days ago)

It's better to go overkill than do the bare minimum, my friend. When you move up to grain and from there up to bulk growing, sterile technique will become something you'll REALLY not want to skimp on.

Losing five pounds of substrate because you felt like being lazy and skipping a few steps is like a swift kick in the nutsack for no good reason. Contamination is entirely preventable, so why not prevent it?


--------------------
This post approved by:


Premedman1 said:
:lol: I just shat my pants.


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Invisiblebmiles
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Re: My first grow. [Re: PoisonedV]
    #7872168 - 01/13/08 02:00 AM (16 years, 19 days ago)

it was kinda hard to split up 1cc between the four holes.
also, i'm pretty faded so that probably didn't help.
12cc's in 6 jars, thats not too bad of overkill i dont think.


--------------------
Never go with a hippy to a second location.


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Invisiblebmiles
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Re: My first grow. [Re: bmiles]
    #7872176 - 01/13/08 02:06 AM (16 years, 19 days ago)

i ended up using the tote as a glove box,
i thought it would be better than doing it in open air.
swabbed the inside with alcohol,
wiped down the gloves and shot up.


--------------------
Never go with a hippy to a second location.


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Offlineseg_x
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Re: My first grow. [Re: bmiles]
    #7872331 - 01/13/08 03:57 AM (16 years, 19 days ago)

gloveboxes are not overkill...

... booo to whomever thinks so.


--------------------


My Fall Mushrooms


Edited by seg_x (01/13/08 03:57 AM)


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Offlineburgerpizza
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Re: My first grow. [Re: seg_x]
    #7872354 - 01/13/08 04:42 AM (16 years, 19 days ago)

way to go man. good luck!


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Offlineq pasa
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Re: My first grow. [Re: seg_x]
    #7872355 - 01/13/08 04:43 AM (16 years, 19 days ago)

bmiles I think you did well so far! having more spores in the jars than needed is not bad. gloveboxes rock! have you thought about how to incubate yet? what´s your roomtemperature? 80 degrees are perfect for incubating but actually anything below is alright too. it may just take longer for the substrate to be fully colonized which kind of increases the contamination risk. you can find nice ideas for building incubators on this site!
anyway, good luck!!


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OfflineThe shroomy 1
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Re: My first grow. [Re: SportinStyle]
    #7872382 - 01/13/08 05:13 AM (16 years, 19 days ago)

Quote:

SportinStyle said:
Honestly the glovebox is not really needed. Sterlization is EXTREMELY important, but a glovebox for PFTek is almost overkill.

You would get by just the same by using a non airated area (no breezes) and by spraying lysol on the area you plan on using. Just pressur cook for the right amount of time, and make sure substrate recieves zero outside contact after that. I have never used a glove box, and my rye grow and PFTek both worked or are working fine, without contams.




There is no such thing as "OVER KILL"! Especially with limited funds. I always recommend "Balls Out" STERILE TECHNIQUE. No one here knows what kind of environment he lives in. Having a pet dog around is enough to transport God knows how many contaminants from the outside in. (That's just an example). There is nothing worse than spending the money and time only to find out that Half your jars, (or more), got contaminated because you skimped on sterile technique.


--------------------


AMU Q&A thread.


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Invisibleshaggydogman
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Re: My first grow. [Re: q pasa]
    #7872386 - 01/13/08 05:17 AM (16 years, 19 days ago)

All sounds good. :smile:
Don't go too high with incubation, higher temps favour bacteria more than mycelium. Mid 70s is good.
Gloveboxes / Still air boxes are great! Essential in my mind.

Now go fill that empty syringe up with water and practice your one handed 0.25cc squirt.

I prefer the toy gun grip. :smile: 
Hold your hand like you're 6 and are pretending to make a gun shape. Thumb up, index finger out, other 3 finders clenched up. Look at your palm. Now imaging the 3 fingers clenched have the syringe in them and the thumb is on the plunger. The index finger is just hanging out there for balance and style points!!

Now I wanna see you empty a full 12cc syringe, making all the necessary syringe adjustments as the syringe gets less full, without using your other hand. So that is 48 squirts of .25cc and I want them done in under 2 Min's...
You wanna cultivate these baby's you gotta become a top syringe slinger...now go practice!!!!

Good luck... :smile:


--------------------
Rye -- WBS | Grain LC -- G2G | Bulk -- Monotub | 50/50 -- Late Casing -- A Pinning Strategy
Disclaimer: My opinion is subject to change at short notice subject to but not limited by new information and knowledge being made available.


Edited by shaggydogman (01/13/08 05:19 AM)


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Offlineveda_sticks
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Re: My first grow. [Re: SportinStyle]
    #7872390 - 01/13/08 05:22 AM (16 years, 19 days ago)

Quote:

SportinStyle said:
Honestly the glovebox is not really needed. Sterlization is EXTREMELY important, but a glovebox for PFTek is almost overkill.

You would get by just the same by using a non airated area (no breezes) and by spraying lysol on the area you plan on using. Just pressur cook for the right amount of time, and make sure substrate recieves zero outside contact after that. I have never used a glove box, and my rye grow and PFTek both worked or are working fine, without contams.




This is really bad advice! while yes you have had great success without one, what about the others who used open air inoculations, failed miserebally and came here wondering why, only to be told use a glove box, and when they did bam success rate thru the roof.

Like me who had a 90% failure rate, moved to pressure cooker instead of steam sterilising, stil 90% failure. Then i made a glove box, bam 100% success.

Not everyone has a low spore count in there house. I say a glovebox is essential. Its good practice, especially for those who want to step up and use agar without having to fork out on a flow hood.

This is the kinda of advice that new people will take, then when the get hit hard with failure, and possibly give up. I would rather recomend to be on the safe side and have them succeed well.


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek


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OfflineThe shroomy 1
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Re: My first grow. [Re: shaggydogman]
    #7872393 - 01/13/08 05:26 AM (16 years, 19 days ago)

Quote:

shaggydogman said:
All sounds good. :smile:
Don't go too high with incubation, higher temps favour bacteria more than mycelium. Mid 70s is good.
Gloveboxes / Still air boxes are great! Essential in my mind.

Now go fill that empty syringe up with water and practice your one handed 0.25cc squirt.

I prefer the toy gun grip. :smile: 
Hold your hand like you're 6 and are pretending to make a gun shape. Thumb up, index finger out, other 3 finders clenched up. Look at your palm. Now imaging the 3 fingers clenched have the syringe in them and the thumb is on the plunger. The index finger is just hanging out there for balance and style points!!

Now I wanna see you empty a full 12cc syringe, making all the necessary syringe adjustments as the syringe gets less full, without using your other hand. So that is 48 squirts of .25cc and I want them done in under 2 Min's...
You wanna cultivate these baby's you gotta become a top syringe slinger...now go practice!!!!

Good luck... :smile:




Ha!  That is "The Shit"!  Very nice Shaggy!  :headbanger:


--------------------


AMU Q&A thread.


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OfflineThe shroomy 1
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Re: My first grow. [Re: bmiles]
    #7872398 - 01/13/08 05:29 AM (16 years, 19 days ago)

Quote:

bmiles said:
i ended up using the tote as a glove box,
i thought it would be better than doing it in open air.
swabbed the inside with alcohol,
wiped down the gloves and shot up.




Good for you! That Glove box will be your Best Friend, I promise!


--------------------


AMU Q&A thread.


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OfflineSportinStyle
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Re: My first grow. [Re: The shroomy 1]
    #7872433 - 01/13/08 06:10 AM (16 years, 19 days ago)

I don't know... although I only have one grow under my belt, the man said he was very limited on funds so I tried to give him an alternative. I'd rather have a perlite terrarium than a glovebox anyday if money was tight.

I have 4 dogs, and live in a house where the two people living here (myself and girlfriend) both smoke like chimneys on christmas, yet I have never had any contams. I will never shoot down the use of any materials that work for anyone, but just like when I began growing marijuana, and everyone's like "Make sure your pH is right, blah, blah."

2 years later, I have made 4 harvests of atleast 3-5 ounces per plant without EVER buffering the pH. That is EXCEPTIONAL for a yield.

Idk, you already did it, so I say good for you. Good luck!!


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Invisibleantimatt3r
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Re: My first grow. [Re: bmiles]
    #7872443 - 01/13/08 06:21 AM (16 years, 19 days ago)

lol one syringe for 6 jars...... oops

I usually put the needle on the glass where I can see it, then squirt out just enough to see it come out into the jar, just a tiny push of the plunger most of the time really its probably over 1/4cc but not over 1/2cc for sure.... you should be able to do about 8-10 jars with 12cc spores... please check out liquid culture if you havent already!


--------------------
Everything and anything said by this person or thru this account is completely fictional and/or hypothetical fantasy and should not be taken seriously and do not reflect the views or actions of the account holder.


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OfflineThe shroomy 1
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Re: My first grow. [Re: SportinStyle]
    #7872455 - 01/13/08 06:44 AM (16 years, 19 days ago)

Quote:

SportinStyle said:
I don't know... although I only have one grow under my belt, the man said he was very limited on funds so I tried to give him an alternative. I'd rather have a perlite terrarium than a glovebox anyday if money was tight.

I have 4 dogs, and live in a house where the two people living here (myself and girlfriend) both smoke like chimneys on christmas, yet I have never had any contams. I will never shoot down the use of any materials that work for anyone, but just like when I began growing marijuana, and everyone's like "Make sure your pH is right, blah, blah."

2 years later, I have made 4 harvests of atleast 3-5 ounces per plant without EVER buffering the pH. That is EXCEPTIONAL for a yield.

Idk, you already did it, so I say good for you. Good luck!!




Lol! I totally understand where you're coming from! I did the same exact thing with both my Dro and my casing layer. Ph this and Ph that.. All in all the Ph level thing really took care of itself. I unfortunately have had my fair share of contamination in my past. As a matter of fact, Building my own Flow hood was the single most important thing I did in my experience as a cultivator. If you have not experienced failure and you have 4 dogs and you and your girlfriend smoke like chimneys, All I can say is WOW! Take a look at my gallery. You'll see the contamination folder.


--------------------


AMU Q&A thread.


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OfflineSupplier
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Re: My first grow. [Re: bmiles]
    #7872510 - 01/13/08 07:21 AM (16 years, 19 days ago)

2 cc's isn't that bad. you should be fine. i got 2 8oz jars to get 3/4 colonized with 20cc's in each jar before it got contaminated haha so im sure you'll be fine.


--------------------
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Invisibleshaggydogman
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Re: My first grow. [Re: Supplier]
    #7872516 - 01/13/08 07:33 AM (16 years, 19 days ago)

No 2ccs per jar is fine.
But 6 jars nocc'ed up when you could have had 12!! :smile:


--------------------
Rye -- WBS | Grain LC -- G2G | Bulk -- Monotub | 50/50 -- Late Casing -- A Pinning Strategy
Disclaimer: My opinion is subject to change at short notice subject to but not limited by new information and knowledge being made available.


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Invisiblebmiles
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Re: My first grow. [Re: shaggydogman]
    #7873971 - 01/13/08 03:49 PM (16 years, 19 days ago)

i sucked up some sterile water in the syringe because there were a lot of spores on the inside of the syringe. i shot them into 3 more jars last night. we will see if that works.  my temp should be fine, its winter here so we always have the heater running, and the sun is shinning through the window on them.

i think the glove box was essential, my girl has two cats that run around here all the time, and our carpet isn't exactly the cleanest.  the glove box gave me a little peace of mind, and i'll just buy another tote and some perlite when they are ready to birth.  i'll have the money by then :crazy2:

i think i can see little mycelium if i look very closely in a few of the jars, or maybe this is wishful thanking :smirk:

thanks for the comments guys.


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Invisiblebmiles
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Re: My first grow. [Re: bmiles]
    #7883894 - 01/15/08 03:09 PM (16 years, 17 days ago)

62 hours and still no visible sign of mycelium...
damn i'm so impatient sometimes :sad:


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Invisibleshaggydogman
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Re: My first grow. [Re: bmiles]
    #7884026 - 01/15/08 03:31 PM (16 years, 17 days ago)

Good sign...If there was colonisation now it would more than likly be a contam. My guess is 123 hrs :p

“If we are facing in the right direction, all we have to do is keep on walking.” - Buddhist proverb.


--------------------
Rye -- WBS | Grain LC -- G2G | Bulk -- Monotub | 50/50 -- Late Casing -- A Pinning Strategy
Disclaimer: My opinion is subject to change at short notice subject to but not limited by new information and knowledge being made available.


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Re: My first grow. [Re: shaggydogman]
    #7884052 - 01/15/08 03:36 PM (16 years, 17 days ago)

well then... i'm glad there isn't any colonization.
my impatience is gone.


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Never go with a hippy to a second location.


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Invisiblebmiles
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Re: My first grow. [Re: bmiles]
    #7894475 - 01/17/08 07:18 PM (16 years, 15 days ago)

ok a little update.
so i inoculated on saturday, and it is now thursday.
i see mycelium in one of my jars, but none in the others.
this is still exciting though, my first mycelium.
the jar in question was inoculated with two other jars
that were inoculated 3 hours after my initial 6 jars.
i looked at the syringe and noticed there were a lot of spores
still stuck on the edges, so i sucked up some tap water and
shot as much of the remaining spores as i could into those three jars.
the only problem is, i think it might become contaminated as i wasn't using very sterile practices when i did the three jars.
atleast something is growing i guess, haha.  :grin:


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Never go with a hippy to a second location.


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Invisiblebmiles
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Registered: 12/31/07
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Re: My first grow. [Re: bmiles]
    #7906244 - 01/20/08 03:59 PM (16 years, 12 days ago)

if you are interested in following my grow,
i set up a grow log and will no longer
be posting in this thread.
here is my grow log:
my first grow, pftek with b+ and limited budget


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Never go with a hippy to a second location.


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