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Invisiblebmiles
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Registered: 12/31/07
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My first grow.
    #7870639 - 01/12/08 07:56 PM (16 years, 20 days ago)

ok... so i got my spore syringe in the mail today, and decided i would get the rest of the supplies that i didn't have, so me and my girl drove all around to stores looking for the stuff. i think i got it all, if so i'm going to start the process tonight or tomorrow. i'm going to be using the pf tek method following the lets grow mushrooms dvd, and i also read the psilocybin mushroom handbook.

supplies:
b+


3.5 cubic feet bag of verm (couldn't find a small bag anywhere)


12 8ounce jars


glove box and pot for sterilizing


micropore tape and gloves


brown rice flour


i have a few questions for you guys...
brown rice flour is a bitch to find, so we ended up going to a chinese market and found rice bran, will this work?

when sterilzing, do you keep the micro pore tape on the jars? the jars will have foil on top of course.

also, do the other supplies look adequate? i'm sure i'll have more questions later on.


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Never go with a hippy to a second location.


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OfflineJustice_Fish
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Re: My first grow. [Re: bmiles]
    #7870649 - 01/12/08 07:58 PM (16 years, 20 days ago)

What's with the tape?
And the gloves?


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InvisibleCrasher
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Re: My first grow. [Re: Justice_Fish]
    #7870660 - 01/12/08 08:01 PM (16 years, 20 days ago)

The gloves are for the...glovebox.

Tape for covering inoculation holes.

Right?


--------------------
Give me silence, water, hope;
Give me struggle, iron, volcanoes...


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Invisiblebmiles
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Re: My first grow. [Re: Crasher]
    #7870665 - 01/12/08 08:01 PM (16 years, 20 days ago)

Quote:

Crasher said:
The gloves are for the...glovebox.

Tape for covering inoculation holes.

Right?




you are correct.


--------------------
Never go with a hippy to a second location.


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Invisiblebmiles
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Re: My first grow. [Re: bmiles]
    #7870689 - 01/12/08 08:07 PM (16 years, 20 days ago)

also, should i wash the jars before loading up the mycelium food?
and i found the answer to my question, rice bran does work instead of rice flour.


Edited by bmiles (01/12/08 08:22 PM)


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OfflineSportinStyle
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Re: My first grow. [Re: bmiles]
    #7870761 - 01/12/08 08:25 PM (16 years, 20 days ago)

Honestly the glovebox is not really needed. Sterlization is EXTREMELY important, but a glovebox for PFTek is almost overkill.

You would get by just the same by using a non airated area (no breezes) and by spraying lysol on the area you plan on using. Just pressur cook for the right amount of time, and make sure substrate recieves zero outside contact after that. I have never used a glove box, and my rye grow and PFTek both worked or are working fine, without contams.


--------------------
RIP Heath Ledger


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Invisiblebmiles
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Re: My first grow. [Re: SportinStyle]
    #7870784 - 01/12/08 08:30 PM (16 years, 20 days ago)

alright, i guess i'll use the plastic tote for my fruiting chamber then.
i don't have a pressure cooker, but i have a big enough pot to do 6 jars at a time, i'm going to steam sterilize them like they do in the dvd.


--------------------
Never go with a hippy to a second location.


Edited by bmiles (01/12/08 08:33 PM)


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OfflineCubie
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Re: My first grow. [Re: SportinStyle]
    #7870793 - 01/12/08 08:32 PM (16 years, 20 days ago)

Id recommend a pressure cooker over the pot if you have one handy. If not their expencive but good luck on your first grow make sure you don't skip even the simplest step and you'll do fine.


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Invisiblebmiles
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Re: My first grow. [Re: Cubie]
    #7870811 - 01/12/08 08:35 PM (16 years, 20 days ago)

buying all this stuff left me with no more money, so i'm stuck with the pot for now, but i think it should be fine. thanks for the advice.


--------------------
Never go with a hippy to a second location.


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OfflineCubie
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Re: My first grow. [Re: bmiles]
    #7870823 - 01/12/08 08:40 PM (16 years, 20 days ago)

Believe me I understand I was once in the same position


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Invisiblebmiles
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Re: My first grow. [Re: Cubie]
    #7870825 - 01/12/08 08:40 PM (16 years, 20 days ago)

did you steam sterilize without a PC?


--------------------
Never go with a hippy to a second location.


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OfflineSportinStyle
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Re: My first grow. [Re: bmiles]
    #7870833 - 01/12/08 08:43 PM (16 years, 20 days ago)

If using a pot, put it on medium setting and put on a lid. I boil for about 1 hour and 30 mins, pc for about 1 and 15.

Just like the other guy said, follow the directions to a "t" and you will be golden.

Good luck!


--------------------
RIP Heath Ledger


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Invisibledumbfounded1600
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Registered: 07/29/07
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Re: My first grow. [Re: bmiles]
    #7870856 - 01/12/08 08:48 PM (16 years, 20 days ago)

SportinStyle - Honestly the glovebox is not really needed. Sterlization is EXTREMELY important, but a glovebox for PFTek is almost overkill.

You would get by just the same by using a non airated area (no breezes) and by spraying lysol on the area you plan on using. Just pressur cook for the right amount of time, and make sure substrate recieves zero outside contact after that. I have never used a glove box, and my rye grow and PFTek both worked or are working fine, without contams.


1. You can never be too sterile. Gloves are better then none at which even though you wash your hands bacteria can still surface.
2. Lysol is just compressed ISO and overpriced..just use ISO in a empty spray bottle...works great. :smirk:
3. And make sure your substrate receives zero outside contact after that? It needs Gas Exchange or it'll stall.


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OfflineCubie
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Re: My first grow. [Re: SportinStyle]
    #7870857 - 01/12/08 08:48 PM (16 years, 20 days ago)

I used singirls zip lock tek.


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Offlinefstrainpain
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Re: My first grow. [Re: Cubie]
    #7870925 - 01/12/08 09:02 PM (16 years, 20 days ago)

i would have done without the rest and got a pc. i use regular brown rice grains and put 1/2 a cup in a blender for about 3 minutes. i don't use a glove box or anything just swabs needle with alcohol and flame sterilize between jars i pc em for 60 min and let cool over night. never had a jar get contaminated.


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Invisiblebmiles
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Re: My first grow. [Re: fstrainpain]
    #7871168 - 01/12/08 09:59 PM (16 years, 20 days ago)

i filled up the jars, time to sterilize them in the pot.


--------------------
Never go with a hippy to a second location.


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Invisiblebmiles
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Re: My first grow. [Re: bmiles]
    #7872143 - 01/13/08 01:51 AM (16 years, 20 days ago)

i had twelve jars, but it was my first time using a syringe and also my first time doing this, so i put too much juice into a few of them, so i only inoculated 6 jars. hopefully i'll have better odds anyway, with more spores in each jar, but now all i can do is wait. thanks for the help guys.
-bmiles


--------------------
Never go with a hippy to a second location.


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OfflinePoisonedV
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Re: My first grow. [Re: bmiles]
    #7872150 - 01/13/08 01:54 AM (16 years, 20 days ago)

Holy shit dude thats overkill. Ive only done a few, and hardly any sterilization, but they all made it


--------------------
Lazy...


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OfflineSkeeblix
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Re: My first grow. [Re: PoisonedV]
    #7872163 - 01/13/08 01:59 AM (16 years, 20 days ago)

It's better to go overkill than do the bare minimum, my friend. When you move up to grain and from there up to bulk growing, sterile technique will become something you'll REALLY not want to skimp on.

Losing five pounds of substrate because you felt like being lazy and skipping a few steps is like a swift kick in the nutsack for no good reason. Contamination is entirely preventable, so why not prevent it?


--------------------
This post approved by:


Premedman1 said:
:lol: I just shat my pants.


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Invisiblebmiles
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Re: My first grow. [Re: PoisonedV]
    #7872168 - 01/13/08 02:00 AM (16 years, 20 days ago)

it was kinda hard to split up 1cc between the four holes.
also, i'm pretty faded so that probably didn't help.
12cc's in 6 jars, thats not too bad of overkill i dont think.


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Never go with a hippy to a second location.


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