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OfflineHappyCake
Stranger

Registered: 01/02/08
Posts: 18
Last seen: 16 years, 1 day
The Liquid Culture Jar.
    #7870429 - 01/12/08 07:14 PM (16 years, 20 days ago)

Hello all,

I have read up on creating a liquid culture and it sounds like a very beneficial thing for me to do for my future in mycology. I went out and snagged a number of Dupont Tyvek envelopes from our favorite friendly government run post office, and some Clear RTV from my neighborhood hardware store.

I read around, some people say to use an entire top of tyvek, others say to make a hole, and then cover it in tyvek. I decided the latter, and glued the Tyvek on the upside of the jar lid covering a 1/2 inch hole with an RTV injection port.

My main concern as with most everyone here is contamination. I am assuming that with proper procedure, 1:20 Honey to water mixture and 20 minutes of pressure cooking with foil covering the exposed top, that i will have less to worry about dealing with contamination.

Am I correct in this assumption?

Secondly, is my main concern also about the possibility of the tyvek becoming wet from mixing up the mycellium growth with a broken shard of glass and or extracting mycellium for inoculation. I am using two layers of the tyvek.

Should i be concerned?
If so, what other solutions are there?

I was just inoculating some jars with a friend about a week back and when withdrawing mycellium from his liquid culture, he didn't appear in any apprehension about turning the jar completely upside down. Of course i don't think he was using Tyvek, however it most likely was just polyfill if not tyvek.

Please, give me a hand and put me in the place I should be.
Thanks

~Happycake


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Invisibleflavoraid
now with twicethe ketamine andopiates!
Male

Registered: 12/05/07
Posts: 1,678
Re: The Liquid Culture Jar. [Re: HappyCake]
    #7870638 - 01/12/08 07:56 PM (16 years, 20 days ago)

agar has a awesome tek for jars he uses a canning jar of w.e size makes two holes and has rubber tubing coming out -- usually it's stuffed with polyfil for gas exchange but he can clamp off the tubing and shake the crap out of them or he can clamp one hole remove the polyfil from the other and extract. seems pretty awesome to me!


--------------------
coda said:
imachavel, Man you really need to do some reading, the amount of bullshit you put into almost every single one of your posts is absolutely astounding.


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InvisibleBlutjager
Inhuman
Male

Registered: 06/11/06
Posts: 9,220
Re: The Liquid Culture Jar. [Re: flavoraid]
    #7870760 - 01/12/08 08:25 PM (16 years, 20 days ago)

The Lc link in my Sig is the easiest way to make liquid culture in my opinion,it is essentially Agar's baby bottle tek with the exception of me liking honey better than karo and I use sharp shards of broken glass as an agitation device


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OfflineHappyCake
Stranger

Registered: 01/02/08
Posts: 18
Last seen: 16 years, 1 day
Re: The Liquid Culture Jar. [Re: Blutjager]
    #7871135 - 01/12/08 09:51 PM (16 years, 20 days ago)

I do like the baby bottle LC, however the main concern brought with that is the air exchange. I would like to avoid stalling out.

~Happycake


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InvisibleDJYoshaBYD
Male

Registered: 04/07/07
Posts: 2,405
Re: The Liquid Culture Jar. [Re: HappyCake]
    #7871149 - 01/12/08 09:54 PM (16 years, 20 days ago)

sterilize at what psi? dont do it too high.. you will caramelize the stuff...20 min at 15 psi works well... imo


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Invisiblekupa trooper
Male User Gallery


Registered: 11/11/07
Posts: 750
Loc: Earth
Re: The Liquid Culture Jar. [Re: DJYoshaBYD]
    #7871263 - 01/12/08 10:23 PM (16 years, 20 days ago)

this is what i made it seems to be working out well. it is not my idea i think it is what flavoraid is talking about. a little involved but worth it IMO.......


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I don't know but I've been told. It's hard to run with the weight of gold                                                                  On the other hand I heard it said. It's just as hard with the weight of lead.


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Invisibleflavoraid
now with twicethe ketamine andopiates!
Male

Registered: 12/05/07
Posts: 1,678
Re: The Liquid Culture Jar. [Re: kupa trooper]
    #7871787 - 01/13/08 12:23 AM (16 years, 20 days ago)

more or less agar used dual rubber tubing and both tubes could serve as inoculation, extraction and gas exchange. I'm going to make 6 pint sized lc jars for my choiced strains and make a dozen baby bottles for w.e -- gotta find the right baby bottles :frown:


--------------------
coda said:
imachavel, Man you really need to do some reading, the amount of bullshit you put into almost every single one of your posts is absolutely astounding.


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InvisibleBlutjager
Inhuman
Male

Registered: 06/11/06
Posts: 9,220
Re: The Liquid Culture Jar. [Re: HappyCake]
    #7871844 - 01/13/08 12:34 AM (16 years, 20 days ago)

Quote:

HappyCake said:
I do like the baby bottle LC, however the main concern brought with that is the air exchange. I would like to avoid stalling out.

~Happycake




Trust me it will get plenty thick enough >> http://www.shroomery.org/forums/showflat.php/Cat/0/Number/7871322/an/0/page/0


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Invisible04281969
Hobbyist
Male User Gallery


Registered: 08/09/06
Posts: 1,406
Re: The Liquid Culture Jar. [Re: Blutjager]
    #7872396 - 01/13/08 05:28 AM (16 years, 19 days ago)

Fantastic! No need to try to reinvent the wheel.
That looks perfect.


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