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blood4blood
Calmer Than You Are


Registered: 04/25/07
Posts: 6,029
Loc: The Valley
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does mycelium water not work on brf cakes???
#7869441 - 01/12/08 03:26 PM (16 years, 20 days ago) |
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i knocked up 6 or so brf cakes on the 5th of january with some mycelium water that i made out of a perfectly fine colonized rye jar following agar's tek just to see if it worked and i have 0% colonization on any of them.
there is plenty of mycelium floating around in the jar and the syringe had visible strands of mycelium in it also (ill try and post a pic of the jar tomorrow)
now i know that's not too terribly long ago but i inoculated 10 brf jars on the 8th with a multispore syringe and they are already 50% colonized. i thought this stuff was supposed to be way faster than ms innoculations.
whats the deal? does this stuff not work on brf cakes? Should i try some of it on some rye? or do you guys think its not viable?
thanks for the help in advance.
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Supplier
Mad Hatter



Registered: 01/05/08
Posts: 705
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Re: does mycelium water not work on brf cakes??? [Re: blood4blood]
#7869452 - 01/12/08 03:28 PM (16 years, 20 days ago) |
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what exactly is mycelium water? is it the liquid that is inside a colonized jar?
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blood4blood
Calmer Than You Are


Registered: 04/25/07
Posts: 6,029
Loc: The Valley
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Re: does mycelium water not work on brf cakes??? [Re: Supplier]
#7869478 - 01/12/08 03:37 PM (16 years, 20 days ago) |
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http://www.shroomery.org/forums/showflat.php?Cat=0&Number=6886318&page=0&vc=&PHPSESSID=#Post6886318
this is the tek. its supposed to be easy as hell to make (whitch it was) and its supposed to work like a liquid culture. meaning it goes to work very fast colonizing because its a living mycelium network already.
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blood4blood
Calmer Than You Are


Registered: 04/25/07
Posts: 6,029
Loc: The Valley
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Re: does mycelium water not work on brf cakes??? [Re: blood4blood]
#7870759 - 01/12/08 08:24 PM (16 years, 20 days ago) |
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nobody has had any expierence with this?
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dumbfounded1600
Stranger

Registered: 07/29/07
Posts: 2,624
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Re: does mycelium water not work on brf cakes??? [Re: blood4blood]
#7870809 - 01/12/08 08:35 PM (16 years, 20 days ago) |
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POST DELETED
Edited by dumbfounded1600 (01/12/08 09:09 PM)
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DJYoshaBYD


Registered: 04/07/07
Posts: 2,405
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Re: does mycelium water not work on brf cakes??? [Re: dumbfounded1600]
#7870819 - 01/12/08 08:37 PM (16 years, 20 days ago) |
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it sounds like you have way too much moisture or too little.. most likely too much, because you said you saw it floating around in the jar... there should be no standing liquid in your jars... that will for sure hinder growth...
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likwid
Student of theArts


Registered: 12/27/07
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Re: does mycelium water not work on brf cakes??? [Re: blood4blood]
#7871015 - 01/12/08 09:21 PM (16 years, 20 days ago) |
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It's possible that the Liquid Culture you used got contaminated. Contaminants a lot of the time will run through your jars before the myc even grabs hold.
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tahoe
Noob Slayer



Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
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Re: does mycelium water not work on brf cakes??? [Re: likwid]
#7871041 - 01/12/08 09:28 PM (16 years, 20 days ago) |
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b4b, just wait. if it was contaminated you would see a discoloration or smell it. Ther isnt too much water in the cake jars. It could just be taking its time. Give it a few more days. I am sure the mycelium would have had to recover a bit from the ass kicking you gave it when you made the mycelium water
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
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RogerRabbit
Bans for Pleasure



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Re: does mycelium water not work on brf cakes??? [Re: tahoe]
#7871051 - 01/12/08 09:31 PM (16 years, 20 days ago) |
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I've been using that method to make syringes for years. Sometimes it doesn't take, but usually it's because I make the slurry too soon. Wait a week or so after full colonization, so the mycelium has a chance to 'bulk up' inside the jar. This way, there will be a lot more that washes off the grains into the water. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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DJYoshaBYD


Registered: 04/07/07
Posts: 2,405
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Re: does mycelium water not work on brf cakes??? [Re: tahoe]
#7871057 - 01/12/08 09:32 PM (16 years, 20 days ago) |
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no way... its been a week and no growth at all from LC.. no way... I get noticeable results within that.. no growth after 7 days with lc means something is wrong... correct me if im wrong
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thedefone
deus ex machina

Registered: 10/06/07
Posts: 1,883
Loc: Gondwana
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Re: does mycelium water not work on brf cakes??? [Re: RogerRabbit]
#7871100 - 01/12/08 09:42 PM (16 years, 20 days ago) |
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I had never seen this particular tek before. Thank you for fucking up! Just kidding, Blood4, just kidding. RR makes a good point. How colonized was the jar you used to do this with? Also, you may just need to wait a little longer, because you're adding that much more moisture?
Thanks though, I've got 40 new PF jars waiting, not enough spores, and 4 FULLY colonized rye jars just laying around. I needed that tek thrown in my face.
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I am become death, the destroyer of worlds.
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blood4blood
Calmer Than You Are


Registered: 04/25/07
Posts: 6,029
Loc: The Valley
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Re: does mycelium water not work on brf cakes??? [Re: DJYoshaBYD]
#7871397 - 01/12/08 10:58 PM (16 years, 20 days ago) |
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Quote:
DJYoshaBYD said: it sounds like you have way too much moisture or too little.. most likely too much, because you said you saw it floating around in the jar... there should be no standing liquid in your jars... that will for sure hinder growth...
sorry for the misunderstnading. but what i meant to say here was, there was alot of mycelium floating around in the lc jar not the brf jar.
im pretty sure that i probabally made the myc water right when i noticed the jar being fully colonized. im defiently going to make another one and ill wait longer after full colonization before i attempt to make it.
ill give these jars a few more days and if i dont see any results ill just re-pc them and start over.
thanks for the help guys
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Blutjager
Inhuman


Registered: 06/11/06
Posts: 9,220
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Re: does mycelium water not work on brf cakes??? [Re: blood4blood]
#7871805 - 01/13/08 12:26 AM (16 years, 19 days ago) |
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If you shoot a syringe full of that stuff into a honey water solution it will get so thick that no matter how much you shake it(Even with broken glass in it) you will have trouble getting it back into a syringe
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blood4blood
Calmer Than You Are


Registered: 04/25/07
Posts: 6,029
Loc: The Valley
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Re: does mycelium water not work on brf cakes??? [Re: Blutjager]
#7872098 - 01/13/08 01:33 AM (16 years, 19 days ago) |
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thats what i heard. i think i might try that just to see if what i have is still viable.
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The shroomy 1
Luminous beings surround me




Registered: 03/27/07
Posts: 5,543
Loc: The Aether
Last seen: 5 months, 5 days
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Re: does mycelium water not work on brf cakes??? [Re: blood4blood]
#7872389 - 01/13/08 05:21 AM (16 years, 19 days ago) |
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The mycelium water didn't work for me either B4B! Don't feel bad. I guess it's like RR said, sometimes it takes and sometimes it doesn't. After that failure, I stuck with Grain to Grain transfers. G2G has never disappointed me.
--------------------
AMU Q&A thread.
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blood4blood
Calmer Than You Are


Registered: 04/25/07
Posts: 6,029
Loc: The Valley
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Re: does mycelium water not work on brf cakes??? [Re: The shroomy 1]
#7872644 - 01/13/08 08:54 AM (16 years, 19 days ago) |
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Quote:
The shroomy 1 said: The mycelium water didn't work for me either B4B! Don't feel bad. I guess it's like RR said, sometimes it takes and sometimes it doesn't. After that failure, I stuck with Grain to Grain transfers. G2G has never disappointed me.
do you have a flowhood? i was thinkong of doing this too but all i have is a glovebox.
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The shroomy 1
Luminous beings surround me




Registered: 03/27/07
Posts: 5,543
Loc: The Aether
Last seen: 5 months, 5 days
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Re: does mycelium water not work on brf cakes??? [Re: blood4blood]
#7872655 - 01/13/08 09:00 AM (16 years, 19 days ago) |
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Yes, I have a flow hood. Although, you can use a glove box with the same results. The only thing the Flow hood did for me was give me Freedom. Freedom of the constrains of that little glove box area.
--------------------
AMU Q&A thread.
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Supplier
Mad Hatter



Registered: 01/05/08
Posts: 705
Loc: The Sky
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Re: does mycelium water not work on brf cakes??? [Re: The shroomy 1]
#7872851 - 01/13/08 10:34 AM (16 years, 19 days ago) |
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well it looks like im not going for the myc water then haha. But it looks like it would be so promisinggg :[[[
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DJYoshaBYD


Registered: 04/07/07
Posts: 2,405
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Re: does mycelium water not work on brf cakes??? [Re: Supplier]
#7872869 - 01/13/08 10:40 AM (16 years, 19 days ago) |
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ahhh... yo comprendo
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blood4blood
Calmer Than You Are


Registered: 04/25/07
Posts: 6,029
Loc: The Valley
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Re: does mycelium water not work on brf cakes??? [Re: DJYoshaBYD]
#7874007 - 01/13/08 03:58 PM (16 years, 19 days ago) |
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ok so still no signs of life in these 7 jars i knocked up with myc water.
would i be able to shoot some spores into these without re-sterilizing them?
i opened one and smelt it. they still smell good.
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