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Offlinelbtchnlgs
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Trich is ruining my life!
    #7864060 - 01/11/08 09:17 AM (16 years, 21 days ago)

I just threw out 3 of 4 cakes. This is my 2nd grow in a row of loosing everything. My 4th cake is has a little bit of trich but im going to keep it around (my apartment is obviously already filled with trich spores) and hope I can get a few fruits.

This is very discouraging, I have not been able to indulge in any fruits in months!

Advice for beginners, KNOW WHAT THE BEGINNING OF TRICH LOOKS LIKE, when you see signs of it TOSS YOUR MATERIAL so you don't end up ruining your ability to grow in the future, as I have.

QUESTION: If I made a mono tub in a decently sterilized enviornment (very small room lysoled heavily) would my risks of contam fall because I would not have to fan?


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OfflineBeebo
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Re: Trich is ruining my life! [Re: lbtchnlgs]
    #7864493 - 01/11/08 11:16 AM (16 years, 21 days ago)

I've never been one for open air innoc. Every time I did, I ended up contaminated, yet every time I use a still air box, no problem. It gets discouraging I know, but just review your sterile tek and everything will work out. Do you flame your needle and keep it wrapped in a alcohol pad?


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Offlinelbtchnlgs
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Re: Trich is ruining my life! [Re: Beebo]
    #7865232 - 01/11/08 02:10 PM (16 years, 21 days ago)

None of my jars get contams, I am very good about that, I use wbs (btw).

It's after I spawn to my coir/coffee that contams show up. It's hard to keep a sterile environment when working with baking trays due to the size


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OfflineRogerRabbitM
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Re: Trich is ruining my life! [Re: lbtchnlgs]
    #7866038 - 01/11/08 05:17 PM (16 years, 21 days ago)

If you're pasteurizing the coir and it contaminates prior to first flush, then it's a fact that the contamination is coming from your spawn. Pasteurized coir can sit damp in open air for weeks without growing molds. Mold spores such as Trichoderma have a hard time germinating on it. That indicates your grain spawn or brf cake is where the mold is originating.
RR


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Offlinelbtchnlgs
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Re: Trich is ruining my life! [Re: RogerRabbit]
    #7866423 - 01/11/08 06:53 PM (16 years, 21 days ago)

RR so your saying that although my apt may be filled with airborne trich spores they should NOT germinate in my coir spawn, even if the bricks have bee sitting in my kitchen cabinet for a few weeks (dry).

I do not see how they could have come from my wbs jars because they were definitely 100% white myc, so it must be my spawn procedure.

When I mix my spawn (coir) I just use boiling water, I will pasteurize next time.

Also, shouldn't I worry about airborne trich and other molds during my daily fanning, or is the myc established enough to fight the contam?

Again, a pointless thanks for all of your help in these forums, since you must be thanked countless times


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OfflineNem
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Re: Trich is ruining my life! [Re: lbtchnlgs]
    #7867011 - 01/11/08 09:59 PM (16 years, 21 days ago)

I am wondering about the coming from wbs jars that are 100% white as well.


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OfflineRogerRabbitM
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Re: Trich is ruining my life! [Re: Nem]
    #7869618 - 01/12/08 04:17 PM (16 years, 20 days ago)

Most molds are white as hell. Until that is, they sporulate.

Try this. Moisten up some coir and set it aside for a few weeks. I doubt seriously any molds will be able to germinate on it for at least two weeks, usually more like five or six.

I'm sure the mold was in your spawn. Try the above experiment to verify. Always give your jars of spawn the smell test before using. Even when solid white, a contaminated jar will often smell like soil, rather than fresh mushrooms. If so, it has molds.
RR


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Invisiblelarge_dose
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Re: Trich is ruining my life! [Re: RogerRabbit]
    #7869948 - 01/12/08 05:34 PM (16 years, 20 days ago)

Hmm, this explains why the only contamination problems i have are with trich hitting my casing layer after the first flush.

I've never had my substrate contaminate, but my casing layer always does 2nd or 3rd flush. I use hydrated lime but that doesnt do good long term so i need some oyster shell.

If i substititued coir for my peat would i have less contamination issues just getting rid of the peat ?

I just love my 50/50+ pinset's.


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OfflineRogerRabbitM
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Re: Trich is ruining my life! [Re: large_dose]
    #7871098 - 01/12/08 09:42 PM (16 years, 20 days ago)

I'd suggest increasing air exchange. You don't need a long-term buffer because casing layers don't outlast the effects of the hydrated lime, which will keep your pH good for at least a month.
RR


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semper in excretia sumus solim profundum variat

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Offline0xYg3n
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Re: Trich is ruining my life! [Re: RogerRabbit]
    #7871436 - 01/12/08 11:04 PM (16 years, 20 days ago)

If one was using grain, and parts of the grain were still slightly uncolonized, like say for instance, only 1/2 of the kernel was covered in mycelia would it then be open to infection?


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Offlinelbtchnlgs
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Re: Trich is ruining my life! [Re: 0xYg3n]
    #7871583 - 01/12/08 11:42 PM (16 years, 20 days ago)

I'll give it all another try. I alcohol and flame the needles before each injection. Ah well, I've never had good luck

Going to sprawl mart in the morning for more wbs. I was planning on doing a g2g transfer, maybe i'll try that in half of my jars, and we'll see check results


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Invisiblelarge_dose
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Re: Trich is ruining my life! [Re: lbtchnlgs] * 1
    #7872082 - 01/13/08 01:30 AM (16 years, 19 days ago)

Dont spawn totally uncolonized grains but i've had no problems with a few uncolonized pieces in there.


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InvisibleDontPlay
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Re: Trich is ruining my life! [Re: large_dose]
    #7872493 - 01/13/08 07:10 AM (16 years, 19 days ago)

Well you should remove those small uncolonized areas before casing.


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Offline0xYg3n
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Re: Trich is ruining my life! [Re: DontPlay]
    #7875715 - 01/13/08 09:00 PM (16 years, 19 days ago)

What about trich on horse poo? Would that be bad grain spawn as well or could it be the poo spawn the mold on its own?


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Re: Trich is ruining my life! [Re: 0xYg3n]
    #7875895 - 01/13/08 09:32 PM (16 years, 19 days ago)

If a bulk substrate contaminates with green mold within two to three weeks of spawning, it was the spawn. NEVER use any uncolonized spawn in a bulk project. Allow the jars to fully colonize, and then let sit for a few days so the mycelium can thicken up.
RR


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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
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