Home | Community | Message Board

World Seed Supply
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: North Spore Bulk Substrate   Original Sensible Seeds Bulk Cannabis Seeds   Left Coast Kratom Buy Kratom Extract   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Mushroom-Hut Liquid Cultures

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
InvisibleCrasher
αἱρετίζω
Male User Gallery


Folding@home Statistics
Registered: 03/13/01
Posts: 6,220
Loc: Tardy to the Party
LC to Agar to assess contamination?
    #7861133 - 01/10/08 07:15 PM (16 years, 22 days ago)

Salutations all-

After preparing a few liquid cultures, I began to consider the volume of contaminated LC's cultivators experience, and present the following question:

Granted that taking one culture to agar is creating a risk of contamination, does anyone take Lc's to agar in order to assess their viability before taking it to grain?

Thanks.


--------------------
Give me silence, water, hope;
Give me struggle, iron, volcanoes...


Extras: Filter Print Post Top
Offlineveda_sticks
Cultivator
Male User Gallery


Folding@home Statistics
Registered: 07/29/07
Posts: 14,191
Loc: UK Flag
Last seen: 4 years, 25 days
Re: LC to Agar to assess contamination? [Re: Crasher]
    #7861142 - 01/10/08 07:18 PM (16 years, 22 days ago)

I just make up 1 pf tek jar as a test, if it works out then i use the rest of the syringe.

So far i havnt had a contaminated LC. Though i have had 1 LC look ok but not grow in a substrate.

The agar however could be a good thing.If u did get contamination and myc grew aswell, you still have the option of cleaning up by transfering.


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek


Extras: Filter Print Post Top
OfflineRogerRabbitM
Bans for Pleasure
Male User Gallery


Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 3 days
Trusted Cultivator
OG Cultivator
Re: LC to Agar to assess contamination? [Re: veda_sticks]
    #7861296 - 01/10/08 07:50 PM (16 years, 22 days ago)

If you have agar skills, I wouldn't even bother with LC. An agar plate will darn near fully grow out from spores before you even see a speck of white in an LC. In addition, you can isolate for rhizomorphic growth on agar.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


Extras: Filter Print Post Top
InvisibleCrasher
αἱρετίζω
Male User Gallery


Folding@home Statistics
Registered: 03/13/01
Posts: 6,220
Loc: Tardy to the Party
Re: LC to Agar to assess contamination? [Re: RogerRabbit]
    #7861809 - 01/10/08 09:15 PM (16 years, 22 days ago)

unfortunately, I haven't touched an agar plate in 7 years, but I suppose shaking the rust off old tricks is worth it.

Thanks for the perspectives.


--------------------
Give me silence, water, hope;
Give me struggle, iron, volcanoes...


Extras: Filter Print Post Top
Invisibletahoe
Noob Slayer
Male User Gallery


Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
Trusted Cultivator
Re: LC to Agar to assess contamination? [Re: Crasher]
    #7862227 - 01/10/08 10:16 PM (16 years, 22 days ago)

my new gig is isolationg rhizomorphic mycelium on a plate and putting it to agar. To test the lc I use a pf jar or grain jar. I do not shake the grain jar and i only inoculate one side of the grain jar. I watch it for a week and if it passes the test I shake it. then I watch it. If it has and mold it will blow up once shook. Finnaly I smell it once fully colonized. it takes a few weeks to test for sure but it is worth it.


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread


Extras: Filter Print Post Top
OfflinePr0_X
CultivationLifer
Male User Gallery


Registered: 01/18/06
Posts: 617
Loc: Igloo's and polar bears
Last seen: 16 years, 1 day
Re: LC to Agar to assess contamination? [Re: tahoe]
    #7862279 - 01/10/08 10:26 PM (16 years, 22 days ago)

The best way to to go about propagating huge amounts of spawn would be to start off from spores to agar, isolate out the best growth over 4 or 5 generations and then slice it into small squares (wedges) and add to a jar of rye, then use that rye with the isolated mycelium to spawn as many jars as it can....

Growing out optimum growth takes abit of time but in the long run you know your going to get the best colonization times and fruits, suffer now and laugh later, LC's are way to easy to contam in my opinion and you can never be sure. but all this is just my two cents


--------------------
It's okay to hurt my feelings
cause you know, they're so numb anyway.
but I guess it's what I get
for being to fuckin stupid to stay away - Jake

- Support the FSR at www.fsrcanada.com and www.fsre.nl


Edited by Pr0_X (01/10/08 10:27 PM)


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: North Spore Bulk Substrate   Original Sensible Seeds Bulk Cannabis Seeds   Left Coast Kratom Buy Kratom Extract   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Mushroom-Hut Liquid Cultures


Similar ThreadsPosterViewsRepliesLast post
* 1st Time Agar Psycholek 464 8 03/02/18 05:03 PM
by Tormato
* Do all contaminants have odor? paranoid2 1,153 4 06/24/02 03:37 PM
by Mycelium
* agar
( 1 2 all )
arabmobster 4,456 21 04/11/03 04:10 PM
by arabmobster
* Why grow on agar? stcore 2,012 1 07/29/02 05:03 PM
by bluhoney
* ? re: LB-agar, also seeking the mycologist aka Erliquin Lefty 2,864 3 05/24/04 01:09 PM
by Lefty
* agar question muse_sick 710 7 05/17/04 12:22 AM
by ATWAR
* Post deleted by Moe Howard vampirism 575 3 11/23/02 02:15 PM
by resin
* Help with agar!? noobmyco 166 2 03/02/18 10:27 AM
by elasticaltiger

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
2,146 topic views. 14 members, 140 guests and 34 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.021 seconds spending 0.007 seconds on 14 queries.