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verminute
Stay out of theOTD!


Registered: 11/16/07
Posts: 595
Last seen: 15 years, 10 months
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Re: I'm Totally New [Re: djsethall]
#7868288 - 01/12/08 07:58 AM (16 years, 20 days ago) |
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Quote:
djsethall said: So should I do a LC the snapple bottle tek and use like 1 cc of spore solution and have that grow? Maybe for use in my next batch?
Do you have a link for this Tek? TIA
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djsethall
Dr. Justy



Registered: 01/07/08
Posts: 88
Loc: PNW
Last seen: 2 years, 11 months
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Okee Dokee, got my snapple bottle in the boiler now. I don't have a PC yet, so I have to do a 90 minute boil I guess. Hopefully it works. It was hard to find a pan that fit the snapple bottle, I'll try to find a smaller glass bottle tommorow at work. I'm hoping that I will be able to do a LC to LC culture to keep the strain alive forever. Hopefully RR or Roadkill will read this and tell me if I am doing this right or not. Anyways, gotta give the dog a bath, Later!! Wish me luck!!!
-------------------- Any posts made by me are purely fictional in nature and by no means is anything I say to be taken seriously. I do not grow or condone the growing of anything not legal. Any and all pictures I post are pictures wideley available on the internet and any discussions I am involved in are purely hypothetical or are commentary in nature and should not constitute advice or be considered advice to assist in activities that are deemed illegal.
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Roadkill
Retired Shroomery Mod



Registered: 12/11/01
Posts: 22,674
Loc: Montana
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Re: I'm Totally New [Re: djsethall]
#7868331 - 01/12/08 08:33 AM (16 years, 20 days ago) |
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Quote:
djsethall said:
Hopefully RR or Roadkill will read this and tell me if I am doing this right or not.
don't ask me...
I'm the one that messed up during shooting RR's video!~
Quote:
djsethall said:
I do think I noticed a breech in sterility protocol in the lets grow mushrooms video. About 12 minutes in, Jim sterilizes his hands, shakes up the spores, then grabs the stool he is sitting on and then lights the alcohol burner with a lighter. I count 3 places that contaminants could be. One is the stool, one is the lighter and the third is the lid of the burner. Am I wrong or what?

your good!~
I was nervous and touched things that I normally wouldn't have touched during the inoculation process.
I should have wiped my gloves down with Isopropyl Alcohol after I touched the stool, the lighter and the burner.
none of the jars made in the video contaminated... though. 
tc
-------------------- Laterz, Road Who the hell you callin crazy? You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch! Brainiac said: PM the names with on there names, that means they have mushrooms for sale.
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Blutjager
Inhuman


Registered: 06/11/06
Posts: 9,220
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Quote:
trueShroom said:
Quote:
Lc is a billion times better than spore syringes in my opinion,its not just for faster inoculation,its a great way to make your spores last forever
Cool, could you possibly link the LC tek you use? She'd like to try it to make a good stock of viable mycelium to grow in the future, even though she has another syringe of Hawaiian Strain waiting in my fridge.(Waiting to see if this first one is successful!)
The tek I use is in my Sig
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verminute
Stay out of theOTD!


Registered: 11/16/07
Posts: 595
Last seen: 15 years, 10 months
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Re: I'm Totally New [Re: Blutjager]
#7868397 - 01/12/08 09:05 AM (16 years, 20 days ago) |
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Whooops, I have my signatures turned off.... Thanks.
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Roadkill
Retired Shroomery Mod



Registered: 12/11/01
Posts: 22,674
Loc: Montana
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Re: I'm Totally New [Re: verminute]
#7868414 - 01/12/08 09:13 AM (16 years, 20 days ago) |
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Quote:
verminute said:
Whooops, I have my signatures turned off.... Thanks.
it's in his journal...
here is the link...
http://www.shroomery.org/forums/showflat.php/Cat/0/Number/7320513/an/0/page/0
tc
-------------------- Laterz, Road Who the hell you callin crazy? You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch! Brainiac said: PM the names with on there names, that means they have mushrooms for sale.
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HeadFood
Shroomer



Registered: 12/30/07
Posts: 390
Last seen: 11 years, 1 month
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Re: I'm Totally New [Re: Roadkill]
#7868459 - 01/12/08 09:30 AM (16 years, 20 days ago) |
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sounds like everything is going good for you so far. keep it up and before you know it you're going to have a huge smile across your face
-------------------- Phi Alpha
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djsethall
Dr. Justy



Registered: 01/07/08
Posts: 88
Loc: PNW
Last seen: 2 years, 11 months
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Re: I'm Totally New [Re: Roadkill]
#7868561 - 01/12/08 10:06 AM (16 years, 20 days ago) |
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Quote:
Roadkill said:
Quote:
djsethall said:
Hopefully RR or Roadkill will read this and tell me if I am doing this right or not.
don't ask me...
I'm the one that messed up during shooting RR's video!~

your good!~
I was nervous and touched things that I normally wouldn't have touched during the inoculation process.
I should have wiped my gloves down with Isopropyl Alcohol after I touched the stool, the lighter and the burner.
none of the jars made in the video contaminated... though. 
tc
Whoa, I wasn't trying to get anyone's goat or anything, just wondering if what I saw was what I saw. What about the LC to LC culture, will it work or what? I would love your input Roadkill. I don't have the means as of yet to be doing grain cultures, slant cultures or strain isolation as of yet. All of which are in the works, heck I have a MJ grow 4 years in the planning. I do know that the devil is in the details. Nobody has answered the question yet as to the LC to LC.
-------------------- Any posts made by me are purely fictional in nature and by no means is anything I say to be taken seriously. I do not grow or condone the growing of anything not legal. Any and all pictures I post are pictures wideley available on the internet and any discussions I am involved in are purely hypothetical or are commentary in nature and should not constitute advice or be considered advice to assist in activities that are deemed illegal.
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djsethall
Dr. Justy



Registered: 01/07/08
Posts: 88
Loc: PNW
Last seen: 2 years, 11 months
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Good news, I see growth. 3 and a half days from inoculation and I come home from work and blammo white moldy lookin stuff on most of my injection sites. I am friggin happier than a.....(couldn't think of anything). Thanks All for your input and I'll keep updating this thread, kinda like a journal. I do still have one unanswered question, Can I keep a lc to lc master going or what? I am thinking that my answer is yes so long as I am careful not to contaminate it. My plan is to have 2 or more snapple bottle lc teks going. I never want to pay for spores again, well at least for the B+ strain.
-------------------- Any posts made by me are purely fictional in nature and by no means is anything I say to be taken seriously. I do not grow or condone the growing of anything not legal. Any and all pictures I post are pictures wideley available on the internet and any discussions I am involved in are purely hypothetical or are commentary in nature and should not constitute advice or be considered advice to assist in activities that are deemed illegal.
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04281969
Hobbyist



Registered: 08/09/06
Posts: 1,406
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Re: I'm Totally New [Re: djsethall]
#7877652 - 01/14/08 10:08 AM (16 years, 18 days ago) |
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I will probably try some LC too, but you don't need to pay for spores ever again once you have some mushrooms growing. From a mushroom, you can make a spore print that will keep for at least a year. It's worked for me, anyway.
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djsethall
Dr. Justy



Registered: 01/07/08
Posts: 88
Loc: PNW
Last seen: 2 years, 11 months
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Ok, so I figured out why nobody was answering my LC to LC question. I just got done reading the CREAMCORN TEK and it is super simple. Props to the cornman. Just do a search of LC tek and look for creamcorn's. It is real easy to do and allows for a biopsy of an actual fruited mushroom instead of using a spore print. This also seems like a great way to do a strain isolation. My cakes are about 25% colonized. Once I birth them and fruit them I will be doing the biopsy tek to et a master LC. This hobby is almost as addictive as growing pot. I need a mass of equipment. I am gonna need a decent microscope, petri dishes, agar solutions and such. I would hihly recommend anyone etting involved with this to purchase the whole dvd set that RR puts out. I wish he would have at least touched on LC though. hint, hint !!!
-------------------- Any posts made by me are purely fictional in nature and by no means is anything I say to be taken seriously. I do not grow or condone the growing of anything not legal. Any and all pictures I post are pictures wideley available on the internet and any discussions I am involved in are purely hypothetical or are commentary in nature and should not constitute advice or be considered advice to assist in activities that are deemed illegal.
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djsethall
Dr. Justy



Registered: 01/07/08
Posts: 88
Loc: PNW
Last seen: 2 years, 11 months
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OK... That god damn sporestore, Never use anyone but a shroomery vendor. I ordered before joining the shroomery, so I had no idea. I was enticed by the low prices I guess. I placed the order on 12/31/07 and wow, they shipped yesterday 1/22/08. Speedy Service. My spores from Hawks Eye have already colonized about 97% in the BRF Cakes. Enough of that Rant. I do Have good news, I will be upgrading my lab with a brand new Mirro Matic 16 Qt PC. Its not an All American but at 16 bucks plus shipping, who can bitch? I think I'll be able to PC 7 quart jars at once. My plan is to do a WBS Grain spawn and probably a straw log tek. Few questions though, Once the Straw log is 100% colonized, can I do a dunk? Possibly in a large tote or something? Will that induce fruiting? I'll be hitting up the local mega chain harddware store soon for some totes, I need a FC and want to make an incubator. I have heard pro's and the "doesn't really matter" arguments. I might hold off on the incubator for awhile. I do need a FC like within a week. I'll probably pick up some perlite while I am there also. Let me know if there is anything more I need to do ASAP?
-------------------- Any posts made by me are purely fictional in nature and by no means is anything I say to be taken seriously. I do not grow or condone the growing of anything not legal. Any and all pictures I post are pictures wideley available on the internet and any discussions I am involved in are purely hypothetical or are commentary in nature and should not constitute advice or be considered advice to assist in activities that are deemed illegal.
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djsethall
Dr. Justy



Registered: 01/07/08
Posts: 88
Loc: PNW
Last seen: 2 years, 11 months
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Re: I'm Totally New [Re: djsethall]
#7929373 - 01/25/08 11:31 AM (16 years, 7 days ago) |
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A little update, my jars are 100% colonized now. I am going to wait a few days before birthing them though. It is really hard to wait. I have made my FC and have 16 quarts of perlite to throw in the bottom of the FC after being soaked. I bought two bags of Miracle Grow perlite that says it has some ferts mixed into the perlite. Will this be a problem? RR or Roadkill, any suggestions? Oh yeah, I got a PC on the way. Bought it from a police auction. 16 bucks plus shipping. It is a mirro matic 16 qt jobby. Not all american, but any pc is better than none.
-------------------- Any posts made by me are purely fictional in nature and by no means is anything I say to be taken seriously. I do not grow or condone the growing of anything not legal. Any and all pictures I post are pictures wideley available on the internet and any discussions I am involved in are purely hypothetical or are commentary in nature and should not constitute advice or be considered advice to assist in activities that are deemed illegal.
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verminute
Stay out of theOTD!


Registered: 11/16/07
Posts: 595
Last seen: 15 years, 10 months
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Re: I'm Totally New [Re: djsethall]
#7929980 - 01/25/08 02:00 PM (16 years, 7 days ago) |
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You don't need fert. in perlite... Serves no propuse.
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djsethall
Dr. Justy



Registered: 01/07/08
Posts: 88
Loc: PNW
Last seen: 2 years, 11 months
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OK, got a little impatient on the whole waiting to birth them. Dunk da dunk dunk dunk. They are dunked in the fridge right now. Smelled like shrooms though. Now I can start my next batch. This Time I'll be using LC stuff instead of spores. TTYL
-------------------- Any posts made by me are purely fictional in nature and by no means is anything I say to be taken seriously. I do not grow or condone the growing of anything not legal. Any and all pictures I post are pictures wideley available on the internet and any discussions I am involved in are purely hypothetical or are commentary in nature and should not constitute advice or be considered advice to assist in activities that are deemed illegal.
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verminute
Stay out of theOTD!


Registered: 11/16/07
Posts: 595
Last seen: 15 years, 10 months
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Re: I'm Totally New [Re: djsethall]
#7934473 - 01/26/08 11:18 AM (16 years, 6 days ago) |
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Word, good luck.
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djsethall
Dr. Justy



Registered: 01/07/08
Posts: 88
Loc: PNW
Last seen: 2 years, 11 months
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Re: I'm Totally New [Re: verminute]
#7959384 - 01/31/08 08:11 AM (16 years, 1 day ago) |
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Wow, that is all I can say is wow!!! I have been reading up here on LC a lot and did one with about 1 cc of spore solution left over from my first batch. I still have 1 cc left in the syringe, but I might not ever need it. I did the karo syrup snapple bottle tek first go around and it worked, well ok. I went to my local walgreens and found syringes with a 21 gauge needle for 25 cents. So I sucked up about 10 cc's of LC making sure to get some of the clumps of stuff floating. After noc'ing up 6 jars, I had about 3.5 cc's left. Anyways, I decided to try a light dried malt extract LC. The Karo and the potato dextrose are kinda slow. I also have about 15 lb's of Light DME left over from a batch of Alaskan Amber that I brewed. So I weighed it out and made a 2% solution by weight, pc'd it and let her cool down. Right before I went to bed about 9 last night, I noticed that the snapple bottle was still a little warm but not hot by any means, so I shot her up with the remaining 3.5 cc's. Here we go, LC to LC with like 10 hours of time in the bottle.

I am pretty impressed, all those clumps are myc
-------------------- Any posts made by me are purely fictional in nature and by no means is anything I say to be taken seriously. I do not grow or condone the growing of anything not legal. Any and all pictures I post are pictures wideley available on the internet and any discussions I am involved in are purely hypothetical or are commentary in nature and should not constitute advice or be considered advice to assist in activities that are deemed illegal.
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djsethall
Dr. Justy



Registered: 01/07/08
Posts: 88
Loc: PNW
Last seen: 2 years, 11 months
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Re: I'm Totally New [Re: trueShroom]
#7982094 - 02/05/08 10:36 AM (15 years, 11 months ago) |
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Woohoo, my first pinset. Finally, I'll have a good fruitbody to take a clone from. Ther is one bigger one surrounded by a few smaller ones. I would post pics, but photobucket is doing maintenance right now. I just wanted to thank RR and Roadkill. You guys ROCK!!!
Update: pics


Edited by djsethall (02/06/08 07:54 AM)
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