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djsethall
Dr. Justy



Registered: 01/07/08
Posts: 88
Loc: PNW
Last seen: 2 years, 11 months
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I'm Totally New
#7859256 - 01/10/08 12:37 PM (16 years, 22 days ago) |
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OK, This is my second attempt at trying to post. I have no idea as to what to do. I have watched RR vids. I have never actually grown mushrooms. I am interested in growing gourmet mushrooms as well as the "others". What exactly is a laminar flow hood? Can I build one? So far I have made 5 BRF cakes in half pint mason jars. I steamed them for 90 minutes as described in the RR vids. I am awaiting spores from two different places. I ordered from sporestore and then after reading some bad reviews, I ordered from Hawk's Eye (BTW, Great Communication) and should be receiving my syringe in a day or two. When growing master cultures in quart jars, how important is it to use rye berries? Can I use other grains like pearl barley? Please correct me if I am wrong, but does mycellium grow in any grain so long as there are no preservatives? I have many more questions but do not want to jumble this post up more than it already is....
Update, The Hawks Eye Fuggin ROCKS. Mailman showed up today with my spores. Little did he know what I was doing with his little delivery. The Spore syringe had some fuzzy looking black stuff in it. I am thinking that the spores germinated and it was mycelium in the syringe. Anyways, I inoculated my BRF cakes that I hope I made correctly. It was RR's recipe and technique to sterilize the cakes. The jars are now sitting on my cable box which sits at 80 degrees 24/7. I should see something in what 3-5 days? I flame sterilized the needle in between jars, not between holes. I ended up using about 7 cc's between 5 jars. I wore rubber gloves and used purell on them in between innoculations. Have I done it right or what? Any feedback?
-------------------- Any posts made by me are purely fictional in nature and by no means is anything I say to be taken seriously. I do not grow or condone the growing of anything not legal. Any and all pictures I post are pictures wideley available on the internet and any discussions I am involved in are purely hypothetical or are commentary in nature and should not constitute advice or be considered advice to assist in activities that are deemed illegal.
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MYSTIQUE
Say Hi to the elves for me.




Registered: 04/28/07
Posts: 1,764
Loc: Canada
Last seen: 5 years, 4 months
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Re: I'm Totally New [Re: djsethall]
#7859275 - 01/10/08 12:43 PM (16 years, 22 days ago) |
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lol I just seen this in the journal. If you are new a flow hood can be a bitch much they are like 250$ and more and kinda hard to build. Id go with a still air box for now. Allot of people around here call them glove boxes.
You can use grains some have different levels of success but if you put the name of your grain you want to use in a search you will get lots of answers from people who have used it. Most people use rye barries wild bird seed finch seed and to less success corn and cracked corn.
Don't count on 3-5 days it takes longer sometimes. Just sit back and wait that what this is all about.
You seemed to do everything fine and clean I don't flame between holes in a jar cuz if one is contaminated they all are in that jar.
-------------------- Dont know what the fuck I just said? READ THIS http://www.shroomery.org/5122/The-Shroomery-Mushroom-Glossary I ain't a hippy but I'm covered in dirt Sippin lots of mushroom tea in a tye-dye shirt Chasin' the Grateful Dead, no shoes on my feet Beggin' in the parking lot for something to eat, DO NOT USE FIRE IN YOUR GLOVE BOX!!!!!!!
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doc34
Fungitarian



Registered: 02/14/04
Posts: 2,667
Loc: Myceliaville !!!
Last seen: 7 months, 29 days
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Re: I'm Totally New [Re: djsethall]
#7859321 - 01/10/08 12:57 PM (16 years, 22 days ago) |
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Quote:
djsethall said: OK, This is my second attempt at trying to post. I have no idea as to what to do. I have watched RR vids. I have never actually grown mushrooms. I am interested in growing gourmet mushrooms as well as the "others". What exactly is a laminar flow hood? Can I build one? So far I have made 5 BRF cakes in half pint mason jars. I steamed them for 90 minutes as described in the RR vids. I am awaiting spores from two different places. I ordered from sporestore and then after reading some bad reviews, I ordered from Hawk's Eye (BTW, Great Communication) and should be receiving my syringe in a day or two. When growing master cultures in quart jars, how important is it to use rye berries? Can I use other grains like pearl barley? Please correct me if I am wrong, but does mycellium grow in any grain so long as there are no preservatives? I have many more questions but do not want to jumble this post up more than it already is....
Update, The Hawks Eye Fuggin ROCKS. Mailman showed up today with my spores. Little did he know what I was doing with his little delivery. The Spore syringe had some fuzzy looking black stuff in it. I am thinking that the spores germinated and it was mycelium in the syringe. Anyways, I inoculated my BRF cakes that I hope I made correctly. It was RR's recipe and technique to sterilize the cakes. The jars are now sitting on my cable box which sits at 80 degrees 24/7. I should see something in what 3-5 days? I flame sterilized the needle in between jars, not between holes. I ended up using about 7 cc's between 5 jars. I wore rubber gloves and used purell on them in between innoculations. Have I done it right or what? Any feedback?
For some-one who has never grown mushrooms you are definately on the right track. Make a glove-box they are cheaper and keep studying. Patience
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When growing master cultures in quart jars, how important is it to use rye berries?
You can use just about any grain, although rye, millet and WBS(wheat,milo,millet) are the most common.
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but does mycellium grow in any grain so long as there are no preservatives?
Most of them yes
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The Spore syringe had some fuzzy looking black stuff in it. I am thinking that the spores germinated and it was mycelium in the syringe.
You would be correct
Quote:
Have I done it right or what? Any feedback?
    
Doc
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djsethall
Dr. Justy



Registered: 01/07/08
Posts: 88
Loc: PNW
Last seen: 2 years, 11 months
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Re: I'm Totally New [Re: doc34]
#7859353 - 01/10/08 01:03 PM (16 years, 22 days ago) |
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About how long before I will be able to see visible colonies? I still have to order some petri dishes and agar stuff so I can do strain isoloations for slant cultures. I figure I have some time to get these things. I have seen some mason jar petri dishes already on this site. If I make a few of these, can I inoculate a petri dish with my syringe and use that to grow some of the mycellium? I'm probably getting way ahead of myself here!!!
Oh yeah, Do I keep the masking tape over the holes I drilled in the lids, or do I remove the tape for gas exchange?
-------------------- Any posts made by me are purely fictional in nature and by no means is anything I say to be taken seriously. I do not grow or condone the growing of anything not legal. Any and all pictures I post are pictures wideley available on the internet and any discussions I am involved in are purely hypothetical or are commentary in nature and should not constitute advice or be considered advice to assist in activities that are deemed illegal.
Edited by djsethall (01/10/08 01:07 PM)
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verminute
Stay out of theOTD!


Registered: 11/16/07
Posts: 595
Last seen: 15 years, 10 months
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Re: I'm Totally New [Re: djsethall]
#7859495 - 01/10/08 01:29 PM (16 years, 22 days ago) |
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It can take up to 2 weeks to see growth... Depending on the species as well. Nice job, sounds like you're on the right track.
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verminute
Stay out of theOTD!


Registered: 11/16/07
Posts: 595
Last seen: 15 years, 10 months
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Re: I'm Totally New [Re: djsethall]
#7859503 - 01/10/08 01:30 PM (16 years, 22 days ago) |
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Quote:
djsethall said: Oh yeah, Do I keep the masking tape over the holes I drilled in the lids, or do I remove the tape for gas exchange?
Yes, remove the tape after incoulation.
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djsethall
Dr. Justy



Registered: 01/07/08
Posts: 88
Loc: PNW
Last seen: 2 years, 11 months
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Re: I'm Totally New [Re: verminute]
#7860865 - 01/10/08 06:17 PM (16 years, 22 days ago) |
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The species is the B plus strain.
-------------------- Any posts made by me are purely fictional in nature and by no means is anything I say to be taken seriously. I do not grow or condone the growing of anything not legal. Any and all pictures I post are pictures wideley available on the internet and any discussions I am involved in are purely hypothetical or are commentary in nature and should not constitute advice or be considered advice to assist in activities that are deemed illegal.
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Funkatron9000
Astronaut



Registered: 10/03/07
Posts: 535
Loc: Sector Zed: Region 6: Sol...
Last seen: 2 years, 2 months
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Re: I'm Totally New [Re: djsethall]
#7860900 - 01/10/08 06:25 PM (16 years, 22 days ago) |
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just wanted to welcome you djsethall! You'll love B+, or atleast I do 
 
-------------------- You cannot yet touch my daughters awesome boobage
You must fight to the death in the Breastriary of Nippopolis.
I thought Gene Wilder was cool BEFORE he was dead.
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verminute
Stay out of theOTD!


Registered: 11/16/07
Posts: 595
Last seen: 15 years, 10 months
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God damn that is a huge flush(s)
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veda_sticks
Cultivator




Registered: 07/29/07
Posts: 14,191
Loc: UK
Last seen: 4 years, 25 days
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Re: I'm Totally New [Re: verminute]
#7861070 - 01/10/08 07:02 PM (16 years, 22 days ago) |
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Quote:
Quote:
The Spore syringe had some fuzzy looking black stuff in it. I am thinking that the spores germinated and it was mycelium in the syringe.
You would be correct
wrong, what you are seeing is thousands and thousands of spores sticking together in a clump, this happens over time, the spores settle and stick to each other. Give the syringe a good shake bofore inoculation to help distrubute the spores in the water.
You are on the right track, asking good questions, infact rather unique questions. Its quite refreshing having a newbie come here and not ask the same boring old questions
-------------------- PF TEK - writeup by EvilMushroom666 Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD Cakes can and will pin! - So you think cakes suck for pins. Your wrong Franks Simple Coir/Verm Tek Franks Proper Pasturisation Tek Franks Spawning To Bulk - Monotub Professor Pinheads RTV Injection Port Tek Foo Mans No Soak WBS Prep Tek
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doc34
Fungitarian



Registered: 02/14/04
Posts: 2,667
Loc: Myceliaville !!!
Last seen: 7 months, 29 days
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Quote:
The Spore syringe had some fuzzy looking black stuff in it. I am thinking that the spores germinated and it was mycelium in the syringe.
--------------------------------------------------------------------------------
You would be correct
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wrong, what you are seeing is thousands and thousands of spores sticking together in a clump, this happens over time, the spores settle and stick to each other. Give the syringe a good shake bofore inoculation to help distrubute the spores in the water.
Me wrong? I highly doubt that-He said "The Spore syringe had some fuzzy looking black stuff in it", "I am thinking that the spores germinated and it was mycelium in the syringe" If you see a dark clump that is "FUZZY" looking, then yes your spores have germinated, if you just see a clump of dark things and no "FUZZY" stuff then, no your spores did not germinate. Am I the only one that has had spores germinate in a syringe, no, it happens quite often in mushroom cultivation, hell the last spore syringe I got from a sponsor was germinated( Dark black looking clump that was "FUZZY").
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verminute
Stay out of theOTD!


Registered: 11/16/07
Posts: 595
Last seen: 15 years, 10 months
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Re: I'm Totally New [Re: doc34]
#7864514 - 01/11/08 11:19 AM (16 years, 21 days ago) |
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You two, battle to the death!!!
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04281969
Hobbyist



Registered: 08/09/06
Posts: 1,406
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Re: I'm Totally New [Re: verminute]
#7864601 - 01/11/08 11:33 AM (16 years, 21 days ago) |
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But, doesn't that usually happen with liquid cultures? What does the spore have to "eat" in distilled water?
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04281969
Hobbyist



Registered: 08/09/06
Posts: 1,406
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Re: I'm Totally New [Re: 04281969]
#7864610 - 01/11/08 11:35 AM (16 years, 21 days ago) |
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BTW, if you want to keep them out in the open, you might want to wrap them in foil so they don't start pinning too early from getting too much light.
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djsethall
Dr. Justy



Registered: 01/07/08
Posts: 88
Loc: PNW
Last seen: 2 years, 11 months
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Re: I'm Totally New [Re: 04281969]
#7866676 - 01/11/08 08:21 PM (16 years, 21 days ago) |
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I have them sitting on top of my cable box which sits in a little doorless cabinet. Should they be in total darkness or what? I could wrap them in foil and leave the lid with the holes exposed for gas exchange? The temp is 70 degrees constant as we never turn the box off. What is the max temp for spores? Right now the jars are on a rack on the box because the heat was sitting at 80 degrees plus. Keep in mind that I have never done this before. I do think I noticed a breech in sterility protocol in the lets grow mushrooms video. About 12 minutes in, Jim sterilizes his hands, shakes up the spores, then grabs the stool he is sitting on and then lights the alcohol burner with a lighter. I count 3 places that contaminants could be. One is the stool, one is the lighter and the third is the lid of the burner. Am I wrong or what?
-------------------- Any posts made by me are purely fictional in nature and by no means is anything I say to be taken seriously. I do not grow or condone the growing of anything not legal. Any and all pictures I post are pictures wideley available on the internet and any discussions I am involved in are purely hypothetical or are commentary in nature and should not constitute advice or be considered advice to assist in activities that are deemed illegal.
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djsethall
Dr. Justy



Registered: 01/07/08
Posts: 88
Loc: PNW
Last seen: 2 years, 11 months
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Re: I'm Totally New [Re: djsethall]
#7866712 - 01/11/08 08:33 PM (16 years, 21 days ago) |
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Liquid cultures, I just read a 10ml to 200ml liquid culture recipe for use in a snapple bottle. Seeing as I have about 3 cc's of spore solution left, should I do a LC in case the 5 jars I made don't take, or should I wait a week or two before starting this? The LC colonizes faster doesn't it? Will the mycellium continue to grow in the LC giving me the ability to make more and more doing like a grain to grain culture but more like a LC to LC culture? I don't want to spend another 23$ on another spore syringe. A few of my first crop will go directly to spore prints, then into my tummy hehe. I need to read up a bit more on LC I guess. If anyone can point me in the right direction, I would be most appreciative. Would an organic corn syrup be better than Karo? So many questions!!!!!!
-------------------- Any posts made by me are purely fictional in nature and by no means is anything I say to be taken seriously. I do not grow or condone the growing of anything not legal. Any and all pictures I post are pictures wideley available on the internet and any discussions I am involved in are purely hypothetical or are commentary in nature and should not constitute advice or be considered advice to assist in activities that are deemed illegal.
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trueShroom
Stranger

Registered: 12/26/07
Posts: 13
Last seen: 15 years, 10 months
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Re: I'm Totally New [Re: djsethall]
#7866817 - 01/11/08 09:06 PM (16 years, 21 days ago) |
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Hey, I'm new to the shroomery as well, and my fish just starting with her first grow (and happily seeing major progress) and just wanted to say hi because my fish is going through a lot of the same things and... those questions are the same ones she'd ask!
Do people reccommend doing liquid cultures over spore syringes? What are the advantages to them, aside from faster inoculation (possibly?)?
As for waiting for your mycelium to colonize, my fish just inoculated her first 5 jars on the 29th and didn't see growth until the 5th of January (also the B+ strain) but this was mostly because the first 3 days were at the room temperature here of 70degrees farhenheit and it was 3 days until she could move it into a built incubator at a nice 80, then growth exploded! currently about 50% - 60% colonized.
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Blutjager
Inhuman


Registered: 06/11/06
Posts: 9,220
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Quote:
trueShroom said: Do people reccommend doing liquid cultures over spore syringes? What are the advantages to them, aside from faster inoculation (possibly?)?
I normally make it a policy of mine not to speak to fish but I will make an exception in this case.... Lc is a billion times better than spore syringes in my opinion,its not just for faster inoculation,its a great way to make your spores last forever,I haven't made or bought a spore syringe in over a yr and have enough to last forever providing it remains viable till I can get to use it(I just tested a few bottles of it and more than half of them showed growth within days of shooting them into more lc solution) I cant wait to test out my awesome Texas clone(I cant grow at the moment)
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djsethall
Dr. Justy



Registered: 01/07/08
Posts: 88
Loc: PNW
Last seen: 2 years, 11 months
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Re: I'm Totally New [Re: Blutjager]
#7866960 - 01/11/08 09:50 PM (16 years, 21 days ago) |
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So should I do a LC the snapple bottle tek and use like 1 cc of spore solution and have that grow? Maybe for use in my next batch?
-------------------- Any posts made by me are purely fictional in nature and by no means is anything I say to be taken seriously. I do not grow or condone the growing of anything not legal. Any and all pictures I post are pictures wideley available on the internet and any discussions I am involved in are purely hypothetical or are commentary in nature and should not constitute advice or be considered advice to assist in activities that are deemed illegal.
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trueShroom
Stranger

Registered: 12/26/07
Posts: 13
Last seen: 15 years, 10 months
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Re: I'm Totally New [Re: Blutjager]
#7867006 - 01/11/08 09:58 PM (16 years, 21 days ago) |
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Quote:
Lc is a billion times better than spore syringes in my opinion,its not just for faster inoculation,its a great way to make your spores last forever
Cool, could you possibly link the LC tek you use? She'd like to try it to make a good stock of viable mycelium to grow in the future, even though she has another syringe of Hawaiian Strain waiting in my fridge.(Waiting to see if this first one is successful!)
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