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OfflineJusListen
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Registered: 12/18/07
Posts: 403
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Last seen: 14 years, 3 months
Re: Forgot to finish of jars with dry verm on top of brf mixture...already pc'd [Re: shaggydogman]
    #7963629 - 02/01/08 06:36 AM (16 years, 20 hours ago)

so the contams travel upwards?

so basically, your throwing the idea of flipping the jars over out the window eh?


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Invisibleshaggydogman
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Registered: 04/20/07
Posts: 672
Re: Forgot to finish of jars with dry verm on top of brf mixture...already pc'd [Re: JusListen]
    #7963731 - 02/01/08 07:45 AM (16 years, 19 hours ago)

Er, not throwing any ideas out :smile: Just saying what works for me. :smile:

I use glasses with foil lids. Turning those upside down is a not a great idea, as it would increase the risk of contam by opening a path down the side of the foil. I've never needed to turn them upside down as gas exchange is excellent with foil lids.
Quote:


Fastfred said:
To explain the utility of the foil cap you must first understand that contams fall downward. You do not need an airtight seal and you don't need a filter. What you must do is make sure that there is no path whereby contams can fall downward into your culture.
http://www.shroomery.org/forums/showflat.php/Number/7857880#Post7857880





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Rye -- WBS | Grain LC -- G2G | Bulk -- Monotub | 50/50 -- Late Casing -- A Pinning Strategy
Disclaimer: My opinion is subject to change at short notice subject to but not limited by new information and knowledge being made available.


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OfflineHybridprX
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Registered: 01/29/08 Happy 16th Shroomiversary!
Posts: 2,588
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Re: Forgot to finish of jars with dry verm on top of brf mixture...already pc'd [Re: shaggydogman]
    #7963736 - 02/01/08 07:49 AM (16 years, 18 hours ago)

My dog Mr.Moogles has done a few hundred cakes without the dry verm layer in open air with just placing a flame sterilized piece of tinfoil over the punctured tinfoil.

And this is going back two years or so in a drafty mold spore laded basement.

I doubt you will have issues unless your sterility technique sucks but I have faith in you.

Or use the micro poor tape method after each innoc


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Invisibleshaggydogman
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Registered: 04/20/07
Posts: 672
Re: Forgot to finish of jars with dry verm on top of brf mixture...already pc'd [Re: HybridprX]
    #7963754 - 02/01/08 07:56 AM (16 years, 18 hours ago)

I did/do the following.

1. Fill glass with sub
2. put 2 pieces of foil over them
3. pc
4. cool
5. move to glovebox
5. remove top foil lid (putting shinny sides together seems to help here :smile: )
6. Noc through bottom foil lid
7. Put top foil lid back on.

Job done :smile: Bigger cakes :smile: No contams so far :smile:


--------------------
Rye -- WBS | Grain LC -- G2G | Bulk -- Monotub | 50/50 -- Late Casing -- A Pinning Strategy
Disclaimer: My opinion is subject to change at short notice subject to but not limited by new information and knowledge being made available.


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Offlinejsin

Registered: 06/26/07
Posts: 46
Loc: Iowa
Last seen: 11 years, 28 days
Re: Forgot to finish of jars with dry verm on top of brf mixture...already pc'd [Re: shaggydogman]
    #8283969 - 04/14/08 08:26 PM (15 years, 9 months ago)

I forgot to put the dry barrier on one of my jars and it was 100% colonized in a week, without contams. The others took normal time (about 4-6 weeks).

My conditions at home are optimal. I usually keep a clean house anyway, but before I do a batch I clean the house well, myself, my supplies and I'm as clean and careful as possible when preparing my jars.

For my next batch I plan on skipping the dry barrier on all jars and look forward to seeing pins in half the time.


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