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Offlineitsallnines
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Registered: 04/17/07
Posts: 385
Last seen: 10 years, 3 months
after three weeks, nothing germinated...
    #7855748 - 01/09/08 07:49 PM (16 years, 23 days ago)

as the title says. i inoculated from a spore print and waited three weeks. nothing grew. nothing at all. no mycelium, no contams. nada.

so, opened a jar and fished around inside. still moist and nothing growing in the middle. i just don't get it.

what really puzzles me is that no contams grew in any of my jars after three weeks. i've done this twice before and always lost at least a couple of jars. of course, this is the first time i've had a pressure cooker. could it really make that much difference? any ideas on why nothing grew?


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by their fruits ye shall know them.


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Offliner311ik
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Registered: 01/24/06
Posts: 192
Loc: Mi
Last seen: 15 years, 1 month
Re: after three weeks, nothing germinated... [Re: itsallnines]
    #7855833 - 01/09/08 08:07 PM (16 years, 23 days ago)

did you let the jars cool first?

theyre not incubating in a cold room are they?


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Invisiblefastfred
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Registered: 05/17/04
Posts: 6,899
Loc: Dark side of the moon
Re: after three weeks, nothing germinated... [Re: r311ik]
    #7855923 - 01/09/08 08:24 PM (16 years, 23 days ago)

> any ideas on why nothing grew?

Unviable spores most likely.


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Offlineitsallnines
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Registered: 04/17/07
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Last seen: 10 years, 3 months
Re: after three weeks, nothing germinated... [Re: fastfred]
    #7856067 - 01/09/08 08:51 PM (16 years, 23 days ago)

jars cooled overnight. jars are at approximately 76F.

i agree about the spores. what really intrigues me is that NOTHING grew. after three weeks, i expected there to be contaminates.


--------------------
by their fruits ye shall know them.


Edited by itsallnines (01/09/08 09:36 PM)


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InvisibleBlutjager
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Registered: 06/11/06
Posts: 9,220
Re: after three weeks, nothing germinated... [Re: fastfred]
    #7857345 - 01/10/08 12:36 AM (16 years, 23 days ago)

Quote:

fastfred said:
> any ideas on why nothing grew?

Unviable spores most likely.




Thats my guess as well


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Offlinec0_hush
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Registered: 07/16/06
Posts: 417
Last seen: 12 years, 4 months
Re: after three weeks, nothing germinated... [Re: Blutjager]
    #7857560 - 01/10/08 01:49 AM (16 years, 22 days ago)

bad spores or bad substrate ratio would be my guess..


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