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verminute
Stay out of theOTD!


Registered: 11/16/07
Posts: 595
Last seen: 15 years, 10 months
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Re: Pinning inside jars @ 75% colonization (w/ pics) [Re: verminute]
#7859461 - 01/10/08 01:20 PM (16 years, 22 days ago) |
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Oh yeah, one more question. Since I currently have only 1 cake in the FC I really don't have to worry about FAE as much, correct? Maybe just 2 -4 times a day?
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verminute
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Registered: 11/16/07
Posts: 595
Last seen: 15 years, 10 months
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Re: Pinning inside jars @ 75% colonization (w/ pics) [Re: DontPlay]
#7859467 - 01/10/08 01:22 PM (16 years, 22 days ago) |
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Quote:
DontPlay said: Yeah im contemplating taking mine out of the jar and getting rid of the non colonized bit. It has alot of little pins but they haven't grown any bigger in 2 days.
I'm having GREAT results so far. Just checked, even more pins and that large pin is already grown beyound the cake... I'm happy for a first timer.
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tealeaf
Just Touch It


Registered: 09/21/06
Posts: 2,907
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Re: Pinning inside jars @ 75% colonization (w/ pics) [Re: Fraggin]
#7859773 - 01/10/08 02:14 PM (16 years, 22 days ago) |
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"Temp drop is not a pinning trigger"
not true. temp drop results in the myc ability and need to feed and grow and switches to fruiting mode. its kinda like a flashing sign telling the myc to switch roles and get going
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Fraggin
Multi-Faceted



Registered: 01/05/05
Posts: 8,707
Last seen: 8 years, 3 days
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Re: Pinning inside jars @ 75% colonization (w/ pics) [Re: tealeaf]
#7859780 - 01/10/08 02:16 PM (16 years, 22 days ago) |
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Look at what happens in nature to understand pinning triggers. errr..Triggers pinning, triggers pins? lol.. Trins piggers...
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verminute
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Registered: 11/16/07
Posts: 595
Last seen: 15 years, 10 months
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Re: Pinning inside jars @ 75% colonization (w/ pics) [Re: Fraggin]
#7859787 - 01/10/08 02:17 PM (16 years, 22 days ago) |
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Quote:
Fraggin said: Look at what happens in nature to understand pinning triggers. errr..Triggers pinning, triggers pins? lol.. Trins piggers...
LOL
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Fraggin
Multi-Faceted



Registered: 01/05/05
Posts: 8,707
Last seen: 8 years, 3 days
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Re: Pinning inside jars @ 75% colonization (w/ pics) [Re: Nibin]
#7859815 - 01/10/08 02:22 PM (16 years, 22 days ago) |
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Quote:
Nibin said:
Quote:
Fraggin said:
Quote:
Nibin said:
Quote:
Captain Cubensis said: I would recommend you use that jar for G2G, since it is showing easy fruiting genetics.
CC, tranferring mycelium from one jar to another will not isolate the easy fruiting genetics. A jar inoculated with multispore will contain different portions with different genetics, be they compatible or not)
By doing a G2G from multispore spawn you will not isolate a set of genetics.
Where did you get that idea from?
He didn't say isolate, and though a grain to grain transfer would not isolate a set of genetics, it would minimize the sets that you are working with when compared to a multi-spore inoculation.
I have taken rhizomorphic mycelium from rhizomorphic areas of a grain jar innnoculated with ms, and grown and entire jar of what look like cotton rope. I have done this on numerous occasions. So, I have to disagree with part of your detraction from the advice given.
I fully agree with your answer, and am totally aware that by doing a g2g selecting specific areas of mycelium you will significantly reduce the amount of sets of genetics but...
C) It won't give the desired results 100% of the time. It is up to luck if you managed to isolate the set of genetics you wanted or not. Without more information on WHY he should have done the g2g and what effects it would have that piece of advice was no good.
I agree that i would be lots of luck involved with this kind of method because there would be a chance that you would get two sub-strains (is that the correct term) or two sets of genetics onto your other grain jar or petri and be dealing with the same dynamic of a jar of multiple genetics, but, it can be done. Usually it takes a bit of running and multiple petris or jars of grain, but, I have done it numerous times......
The guys idea was to keep a good colony of mycelium going by doing a grain transfer, not bad advice at all. I pointed out that you detracted from his advice by bringing up isolates. A totally seperate process altogether that wasn't mentioned.
Remember, its more helpful to add advice than to detract from advice, unless someone is blatently wrong of course. I have come to the conclusion that some people in this hobby have to grow some green in order to grow some shrooms and are going to make illogical judgements and follow silly teks just subconciously just to learn.
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Nibin
Getting there



Registered: 11/29/05
Posts: 4,480
Last seen: 10 years, 8 months
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Re: Pinning inside jars @ 75% colonization (w/ pics) [Re: tealeaf]
#7859829 - 01/10/08 02:24 PM (16 years, 22 days ago) |
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Quote:
tealeaf said: "Temp drop is not a pinning trigger"
not true. temp drop results in the myc ability and need to feed and grow and switches to fruiting mode. its kinda like a flashing sign telling the myc to switch roles and get going
With species that grow in colder climates yes, but not with P. Cubensis. They are a tropical species and do not get temp drops in nature so why would they react to a temp drop?
Tests have been made, with cold shocked and non cold shocked cubes side by side and cold shocking them actually slows them down
-------------------- Newcomers guide-----> For all things shroomy
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verminute
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Registered: 11/16/07
Posts: 595
Last seen: 15 years, 10 months
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Re: Pinning inside jars @ 75% colonization (w/ pics) [Re: Nibin]
#7859837 - 01/10/08 02:25 PM (16 years, 22 days ago) |
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As Nibin said, just to verifiy, yes, these are B+ cubes.
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tealeaf
Just Touch It


Registered: 09/21/06
Posts: 2,907
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Re: Pinning inside jars @ 75% colonization (w/ pics) [Re: verminute]
#7860227 - 01/10/08 03:42 PM (16 years, 22 days ago) |
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"Tests have been made, with cold shocked and non cold shocked cubes side by side and cold shocking them actually slows them down "
very interesting. ive just been confused because many teks say to drop temp to initiate fruiting. i just always thought that drop in temp or cold shocking caused the myc to switch gears and start the process of fruiting.
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DontPlay
Yeah Science!



Registered: 12/13/07
Posts: 750
Loc: On Land most of the time....
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Re: Pinning inside jars @ 75% colonization (w/ pics) [Re: tealeaf]
#7860351 - 01/10/08 04:16 PM (16 years, 22 days ago) |
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Maybe the decrease in temp would help trigger but cold shocking a tropical species would probably confuse them. There use to more temperate climate.
-------------------- My Trade List I hate to advocate drugs, alcohol, violence, or insanity to anyone, but they've always worked for me. Hunter S. Thompson
I prefer dangerous freedom over peaceful slavery. ~Thomas Jefferson
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verminute
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Registered: 11/16/07
Posts: 595
Last seen: 15 years, 10 months
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Re: Pinning inside jars @ 75% colonization (w/ pics) [Re: DontPlay]
#7864560 - 01/11/08 11:26 AM (16 years, 21 days ago) |
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I took a picture this morning, but being a camera phone and the fact I was running late it was way too blurry...
But I now have four pins which are huge! So I'm happy...
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Blutjager
Inhuman


Registered: 06/11/06
Posts: 9,220
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Re: Pinning inside jars @ 75% colonization (w/ pics) [Re: Nibin]
#7864897 - 01/11/08 12:49 PM (16 years, 21 days ago) |
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Quote:
Nibin said: Cold shocking them actually slows them down
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verminute
Stay out of theOTD!


Registered: 11/16/07
Posts: 595
Last seen: 15 years, 10 months
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Re: Pinning inside jars @ 75% colonization (w/ pics) [Re: Blutjager]
#7868280 - 01/12/08 07:55 AM (16 years, 20 days ago) |
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Here is my FC - that cake is doing so well I wish I had a better camera...
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DJYoshaBYD


Registered: 04/07/07
Posts: 2,405
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Re: Pinning inside jars @ 75% colonization (w/ pics) [Re: verminute]
#7868426 - 01/12/08 09:19 AM (16 years, 20 days ago) |
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I use a heating pad and a thermostat that you can get from any hydro shop... its used for seedling heating pads... I have used these for years, and they work great... just get a heating pad that is meant to have the lil cusion that you soak in water and put it in the heating pad.. That way you know it is water proof, and the thermostat has a failsafe, so if it shorts, it kills power... very safe.. kinda pricey.. about $45 for the thermostat.. but they last forever, and you can use them in any size terrarium, as long as you have a water proof pad to add heat (or you can use the TiT with the aquarium heater, and hook that to the thermostat.. It has a temp probe that you put in the incubator to watch the temps..)
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verminute
Stay out of theOTD!


Registered: 11/16/07
Posts: 595
Last seen: 15 years, 10 months
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Re: Pinning inside jars @ 75% colonization (w/ pics) [Re: DJYoshaBYD]
#7868681 - 01/12/08 11:10 AM (16 years, 20 days ago) |
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I think you nailed it DJ Yosha... I need to invest, err, upgrade to what you suggested, thanks!
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DontPlay
Yeah Science!



Registered: 12/13/07
Posts: 750
Loc: On Land most of the time....
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Re: Pinning inside jars @ 75% colonization (w/ pics) [Re: verminute]
#7868692 - 01/12/08 11:13 AM (16 years, 20 days ago) |
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Or you could buy two rubbermaid bins and an aquarium heater for cheaper than 45 and make a tit incubator. Plain simple and easy. Using a heating pad distributes the heat unevenly, It might work but it's not the best idea.
-------------------- My Trade List I hate to advocate drugs, alcohol, violence, or insanity to anyone, but they've always worked for me. Hunter S. Thompson
I prefer dangerous freedom over peaceful slavery. ~Thomas Jefferson
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RogerRabbit
Bans for Pleasure



Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 3 days
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Re: Pinning inside jars @ 75% colonization (w/ pics) [Re: DontPlay]
#7868743 - 01/12/08 11:32 AM (16 years, 20 days ago) |
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You guys really need to quit confusing silly 'incubators' with fruiting chambers. First, incubators are not needed if you heat your house to normal room temperature, and secondly, never heat a fruiting chamber. It causes condensation, which robs your humidity right out of the air and turns it into useless moisture on the side of the container, thus drying out your cakes/substrates. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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DontPlay
Yeah Science!



Registered: 12/13/07
Posts: 750
Loc: On Land most of the time....
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Re: Pinning inside jars @ 75% colonization (w/ pics) [Re: RogerRabbit]
#7868800 - 01/12/08 11:49 AM (16 years, 20 days ago) |
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I didn't even realize he was talking about an FC, I was just responding to the comment about the heating pad thing.
-------------------- My Trade List I hate to advocate drugs, alcohol, violence, or insanity to anyone, but they've always worked for me. Hunter S. Thompson
I prefer dangerous freedom over peaceful slavery. ~Thomas Jefferson
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fstrainpain
Stranger

Registered: 01/06/08
Posts: 32
Last seen: 15 years, 11 months
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Re: Pinning inside jars @ 75% colonization (w/ pics) [Re: DontPlay]
#7868888 - 01/12/08 12:29 PM (16 years, 20 days ago) |
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Good point RR i never use incubator my house stayes at 72 i just put my cakes in a big ass lunch box with a quilt my grandmother made
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fstrainpain
Stranger

Registered: 01/06/08
Posts: 32
Last seen: 15 years, 11 months
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Re: Pinning inside jars @ 75% colonization (w/ pics) [Re: fstrainpain]
#7868892 - 01/12/08 12:30 PM (16 years, 20 days ago) |
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well i cover the box with the quilt
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