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Offlineverminute
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Re: Pinning inside jars @ 75% colonization (w/ pics) [Re: Nibin]
    #7854435 - 01/09/08 03:52 PM (16 years, 23 days ago)

Quote:

Nibin said:
PS: Early pinning inside a jar is normally caused by or contamination or an improperly prepared substrate. The mycelium thinks it has finished colonizing all it can (even if there is stuff left in the jar) and starts fruiting.




The cake pictured, as you suggest, does have an area where the substrate appears to be gapped from the canning jar. However the pin is far from this area, and it appears to be the most healthy area of the cake...

Can you clearify 'improperly prepared sustrate'? Or just an example. Thanks again for the response! :smile:


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InvisibleDontPlay
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Re: Pinning inside jars @ 75% colonization (w/ pics) [Re: Fraggin]
    #7854511 - 01/09/08 04:00 PM (16 years, 23 days ago)

well he did say use it for G2G and that is not grain those are BRF cakes. So he is wrong in saying use it for G2G.


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Offlineverminute
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Re: Pinning inside jars @ 75% colonization (w/ pics) [Re: DontPlay]
    #7854522 - 01/09/08 04:01 PM (16 years, 23 days ago)

Oh, I see what you're saying... Yes, these are BRF cakes.


Edited by verminute (01/09/08 04:01 PM)


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InvisibleDontPlay
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Re: Pinning inside jars @ 75% colonization (w/ pics) [Re: verminute]
    #7854541 - 01/09/08 04:04 PM (16 years, 23 days ago)

It can still be done, if you have a Glovebox. I don't recommend doing it without or you'll wait a week and see that your "new jars" have been contaminated from opening the lids after PCing.


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Offlineverminute
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Re: Pinning inside jars @ 75% colonization (w/ pics) [Re: DontPlay]
    #7854552 - 01/09/08 04:07 PM (16 years, 23 days ago)

Makes sense, yup... I have a glove box, but homemade, so I won't risk it. Plus I'm about to move, so I might as well keep the jars in incubation rather than risking contaim.

Again, I can't thank everyone enough for the kind responses.


Edited by verminute (01/09/08 04:08 PM)


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InvisibleCaptain Cubensis
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Re: Pinning inside jars @ 75% colonization (w/ pics) [Re: Nibin]
    #7854608 - 01/09/08 04:18 PM (16 years, 23 days ago)

No, I wasn't implying that would constitute an isolation, using a proven fruiter for G2G.

But I do think most people would use their best jars versus their worst for G2G.

For example, if you knocked up 6 jars, and planned to fruit 3 and use 3 for G2G, which three would you choose for G2G?


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Offlineverminute
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Re: Pinning inside jars @ 75% colonization (w/ pics) [Re: Captain Cubensis]
    #7854624 - 01/09/08 04:20 PM (16 years, 23 days ago)

Logical... totally...

Since I inculated all the jars around the same time and this jar came out best oviously it would be the one picked IMO. I can't say the pin genes are the best, but from what I have it's the winner... If that makes any sense...


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OfflineNibin
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Re: Pinning inside jars @ 75% colonization (w/ pics) [Re: Fraggin]
    #7854891 - 01/09/08 05:12 PM (16 years, 23 days ago)

Quote:

Fraggin said:
Quote:

Nibin said:
Quote:

Captain Cubensis said:
I would recommend you use that jar for G2G, since it is showing easy fruiting genetics.




CC, tranferring mycelium from one jar to another will not isolate the easy fruiting genetics. A jar inoculated with multispore will contain different portions with different genetics, be they compatible or not)

By doing a G2G from multispore spawn you will not isolate a set of genetics.

Where did you get that idea from?






He didn't say isolate, and though a grain to grain transfer would not isolate a set of genetics, it would minimize the sets that you are working with when compared to a multi-spore inoculation.

I have taken rhizomorphic mycelium from rhizomorphic areas of a grain jar innnoculated with ms, and grown and entire jar of what look like cotton rope. I have done this on numerous occasions. So, I have to disagree with part of your detraction from the advice given.




I fully agree with your answer, and am totally aware that by doing a g2g selecting specific areas of mycelium you will significantly reduce the amount of sets of genetics but...

A) I doubt that is the way CC meant it (he'll argue that he did)

B) He didn't go into further detail, which can cause future confusion, especially to noobs.

C) It won't give the desired results 100% of the time. It is up to luck if you managed to isolate the set of genetics you wanted or not. Without more information on WHY he should have done the g2g and what effects it would have that piece of advice was no good.


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Offlineveda_sticks
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Re: Pinning inside jars @ 75% colonization (w/ pics) [Re: Nibin]
    #7855074 - 01/09/08 05:56 PM (16 years, 23 days ago)

Well done to those who took the inititive to search and find out more about my answer.

Cold shocking is sometimes a bit of a misunderstood term. Dunking in the fridge is not to cold shock the mycelium, but to slow down bacteria growth that may be in the water. It isnt essential to dunk in the fridge, but it is considered good practice.

Another justification of temp drop not being a pinning trigger, many cultivators have grown cubes at the same temperatures and even higher than incubation, without noticing difference in pinning.

The 1 thing that temperature will effect is growth speed, obviously. There was a debate a little bit back about temperature and densitiy of fruits. Not sure what the outcome was.


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InvisibleDontPlay
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Re: Pinning inside jars @ 75% colonization (w/ pics) [Re: veda_sticks]
    #7855298 - 01/09/08 06:37 PM (16 years, 23 days ago)

Well I can say that I went threw 2 flushs on a cake and it was in an 80 degree terrarium it ended up having some very skinny stem very tall tho, and matured in 4 days.


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OfflineRogerRabbitM
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Re: Pinning inside jars @ 75% colonization (w/ pics) [Re: DontPlay]
    #7855502 - 01/09/08 07:15 PM (16 years, 23 days ago)

Never use a jar that pins early for grain to grain transfers. Usually, something is the trigger for early pinning, such as bacterial contamination. I know you don't g2g brf jars, but had it been a grain jar, you wouldn't want to use if for transfers.
RR


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InvisibleCaptain Cubensis
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Re: Pinning inside jars @ 75% colonization (w/ pics) [Re: RogerRabbit]
    #7855555 - 01/09/08 07:24 PM (16 years, 23 days ago)

But there are no signs of contams, and it is not early, it's a 1/2 pint. And you can transfer chunks of BRF in a glove box with success if you are clean.

You put a lot of energy into disproving people but little into helpful advice.

By the way Roger, Jimi sang "Actin' funny, but I don't know why, 'scuse me while I kiss the sky."

Not "I feel funny and I don't know why. . .Excuse me while I kiss the sky", so get it right, and don't misquote people.


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OfflineNibin
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Re: Pinning inside jars @ 75% colonization (w/ pics) [Re: Captain Cubensis]
    #7855655 - 01/09/08 07:37 PM (16 years, 23 days ago)

Quote:

Captain Cubensis said:
But there are no signs of contams, and it is not early, it's a 1/2 pint. And you can transfer chunks of BRF in a glove box with success if you are clean.

You put a lot of energy into disproving people but little into helpful advice.

By the way Roger, Jimi sang "Actin' funny, but I don't know why, 'scuse me while I kiss the sky."

Not "I feel funny and I don't know why. . .Excuse me while I kiss the sky", so get it right, and don't misquote people.




How do you know there are no signs of contamination? Are you superman and have supervision? There could be a bacterial contamination.

And RR has many times said that you can transfer pf cakes, especially if you don't use the dry verm barrier and cover the innoc holes in micropore instead.


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InvisibleDontPlay
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Re: Pinning inside jars @ 75% colonization (w/ pics) [Re: Captain Cubensis]
    #7856184 - 01/09/08 09:18 PM (16 years, 23 days ago)

Quote:

Captain Cubensis said:
But there are no signs of contams, and it is not early, it's a 1/2 pint. And you can transfer chunks of BRF in a glove box with success if you are clean.

You put a lot of energy into disproving people but little into helpful advice.

By the way Roger, Jimi sang "Actin' funny, but I don't know why, 'scuse me while I kiss the sky."


Not "I feel funny and I don't know why. . .Excuse me while I kiss the sky", so get it right, and don't misquote people.




Where do you get off saying RR doesn't make any effort into helping people. ARE YOU CRAZY he has more post here than anyone I imagine, and more than half I bet are helping people. Quit acting like a douche bag if you want to stick around. Im sure you can defend yourself without talking shit about the moderators,especially when it's BS.


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Edited by DontPlay (01/09/08 09:19 PM)


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OfflineanarchOi
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Re: Pinning inside jars @ 75% colonization (w/ pics) *DELETED* [Re: Nibin]
    #7856260 - 01/09/08 09:34 PM (16 years, 23 days ago)

Post deleted by anarchOi

Reason for deletion: off topic



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Edited by anarchOi (01/10/08 12:16 AM)


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InvisibleDontPlay
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Re: Pinning inside jars @ 75% colonization (w/ pics) [Re: anarchOi]
    #7856362 - 01/09/08 09:48 PM (16 years, 23 days ago)

That post wasn't talking about early pinning, it had to do with temp. drops being a pinning trigger ...


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I hate to advocate drugs, alcohol, violence, or insanity to anyone, but they've always worked for me. 
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Offlineverminute
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Re: Pinning inside jars @ 75% colonization (w/ pics) [Re: DontPlay]
    #7859057 - 01/10/08 12:03 PM (16 years, 22 days ago)

Update: The jar now has a mushroom, not a pin, a huge shroom about to grow out of the jar last night when I got home... So that was like only 18 hours after the pictures were taken. Even with the cake at 85% colonization I had to birth it. So far so good. As for my FC, it's currently 100% humidity and around 75, 78 degrees F. Do you think I should drop the temps a bit?


Edited by verminute (01/10/08 12:06 PM)


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OfflineRogerRabbitM
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Re: Pinning inside jars @ 75% colonization (w/ pics) [Re: verminute]
    #7859411 - 01/10/08 01:14 PM (16 years, 22 days ago)

No, you're perfect. Did you wash the cake well after birthing to remove any uncolonized parts?
RR


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Offlineverminute
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Re: Pinning inside jars @ 75% colonization (w/ pics) [Re: RogerRabbit]
    #7859444 - 01/10/08 01:18 PM (16 years, 22 days ago)

Quote:

RogerRabbit said:
No, you're perfect. Did you wash the cake well after birthing to remove any uncolonized parts?
RR




The uncolonized parts were white with mycelium, it would've been more trouble with contaim. and detail with a knife than worth.

So basically I can see a little darkness of vermiculite that's not totally covered with mycelium... I know this isn't best method, but I really had no choice, it was ready to fruit!!


Edited by verminute (01/10/08 01:19 PM)


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InvisibleDontPlay
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Re: Pinning inside jars @ 75% colonization (w/ pics) [Re: verminute]
    #7859460 - 01/10/08 01:20 PM (16 years, 22 days ago)

Yeah im contemplating taking mine out of the jar and getting rid of the non colonized bit. It has alot of little pins but they haven't grown any bigger in 2 days.


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