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Offlinekillerthc
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Registered: 11/21/07
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Re inoculation of LC
    #7852489 - 01/09/08 06:23 AM (16 years, 23 days ago)

I made a LC about a week ago and me being so impatient I inoculated it when it was still fairly hot...... i waited fro ages but never saw any growth from either contaminations or mycelium.... so today i re inoculated the LC same as before after sterilizing it again and waiting for it to cool about 24 hours with the same spores and same sterile methods..... My question is will this possibly work?


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OfflineSupplier
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Re: Re inoculation of LC [Re: killerthc]
    #7852672 - 01/09/08 08:32 AM (16 years, 23 days ago)

make sure u just put it in the fridge over night and let it cool this time.


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OfflineMahayana
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Re: Re inoculation of LC [Re: Supplier]
    #7852711 - 01/09/08 09:04 AM (16 years, 23 days ago)

It should , I would just make sure if you do get growth to knock up a test jar and make sure there is no contams. If the test jar is good then inoculate the many that you plan on using, it'll save you alot of work, if its contaminated and you ruin a whole batch of substrate compared to just one test jar. Contams are hard to spot in LC's. Also never put it in the fridge to cool it down faster, let it cool overnight at room temperature. The fridge has lots of harmful bacteria in it and putting a warm lc in there, can cause the warm jar to suck in contaminated air from the fridge. Patience is a good friend with this hobby.


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The river of Zen is quiet,even in the waves;
the water of stability is clear, even in the waves.


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OfflineSupplier
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Re: Re inoculation of LC [Re: Mahayana]
    #7852723 - 01/09/08 09:11 AM (16 years, 23 days ago)

sorry for my post haha i was wrong. listen to him!


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Offlinethe4x4hoss
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Re: Re inoculation of LC [Re: Supplier]
    #7852771 - 01/09/08 09:29 AM (16 years, 23 days ago)

I will second Mahayana's comment... Especially the part about not putting LC in the fridge... If you notice an odd (rotten) smell from your LC you definitely have a case of bacteria contamination from the fridge.

P.S.
Supplier, to save you an ass ripping from Tahoe, it would be wise to not spread disinformation.


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"Heaven is not a place you go when you die, rather it is that moments in time when you actually feel alive." Mushies help...


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Offlinekillerthc
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Re: Re inoculation of LC [Re: the4x4hoss]
    #7853148 - 01/09/08 11:21 AM (16 years, 23 days ago)

thanks everyone ill remember patience is my friend....


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