Home | Community | Message Board

World Seed Supply
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies   PhytoExtractum Kratom Powder for Sale, Maeng Da Thai Kratom Leaf Powder   Bridgetown Botanicals CBD Concentrates

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlineresptodd
I reject yourreality andsubstitute myown
Male User Gallery


Registered: 10/16/07
Posts: 674
Loc: Michigan
Last seen: 1 year, 10 months
cloning
    #7852457 - 01/09/08 05:56 AM (16 years, 23 days ago)

I'm gonna' try and do some cloning. Can someone get me some good links?


--------------------
Damn! I'm having fun! Just keep the GD monkeys away.


Extras: Filter Print Post Top
InvisibleDontPlay
Yeah Science!
Male


Registered: 12/13/07
Posts: 750
Loc: On Land most of the time....
Re: cloning [Re: resptodd]
    #7852476 - 01/09/08 06:11 AM (16 years, 23 days ago)

There is a good link, http://www.shroomery.org/search.php


--------------------
My Trade List


I hate to advocate drugs, alcohol, violence, or insanity to anyone, but they've always worked for me. 
    Hunter S. Thompson



I prefer dangerous freedom over peaceful slavery.
~Thomas Jefferson


Extras: Filter Print Post Top
Offlineresptodd
I reject yourreality andsubstitute myown
Male User Gallery


Registered: 10/16/07
Posts: 674
Loc: Michigan
Last seen: 1 year, 10 months
Re: cloning [Re: DontPlay]
    #7852478 - 01/09/08 06:16 AM (16 years, 23 days ago)

Now that's funny! You win.------No, for real, there is just so much info. out there I was just hoping someone could get me on the right track. I did do a search.


--------------------
Damn! I'm having fun! Just keep the GD monkeys away.


Extras: Filter Print Post Top
Offlineveda_sticks
Cultivator
Male User Gallery


Folding@home Statistics
Registered: 07/29/07
Posts: 14,191
Loc: UK Flag
Last seen: 4 years, 25 days
Re: cloning [Re: resptodd]
    #7852480 - 01/09/08 06:17 AM (16 years, 23 days ago)

check my signiture out.

I have heard a lot of sucecss with this, and alot of failure. Getting a clean sample is very important.


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek


Extras: Filter Print Post Top
Offlineresptodd
I reject yourreality andsubstitute myown
Male User Gallery


Registered: 10/16/07
Posts: 674
Loc: Michigan
Last seen: 1 year, 10 months
Re: cloning [Re: veda_sticks]
    #7852494 - 01/09/08 06:26 AM (16 years, 23 days ago)

VS thanx, just what I was looking for. But, are you always sure to get a sample when poke the 'shroom?


--------------------
Damn! I'm having fun! Just keep the GD monkeys away.


Extras: Filter Print Post Top
Invisibleshroober
Myco Junkie


Registered: 01/02/08
Posts: 879
Re: cloning [Re: DontPlay]
    #7852546 - 01/09/08 07:05 AM (16 years, 23 days ago)

Quote:

DontPlay said:
There is a good link, http://www.shroomery.org/search.php




This is getting really old. All he is asking is what cloning technique others suggest and links to some preferred teks on the site. It's a totally legitimate question. Stop assuming everyone that asks a question hasnt tried searching. Should we really only rely on previously posted information? If that's the case you might as well lock the forums and only allow searching. If you don't have anything informative to add to the topic then don't reply. Simple as that.


Extras: Filter Print Post Top
OfflineNibin
Getting there
Male User Gallery


Registered: 11/29/05
Posts: 4,480
Last seen: 10 years, 8 months
Trusted Cultivator
Re: cloning [Re: resptodd]
    #7852589 - 01/09/08 07:32 AM (16 years, 23 days ago)

Quote:

resptodd said:
VS thanx, just what I was looking for. But, are you always sure to get a sample when poke the 'shroom?




Use a wide gauge needle to make sure you get the sample.


--------------------
Newcomers guide-----> For all things shroomy


Extras: Filter Print Post Top
Invisibleuncle_rico
my own worst enemy
Male User Gallery

Registered: 03/28/06
Posts: 2,664
Re: cloning [Re: resptodd]
    #7853031 - 01/09/08 10:49 AM (16 years, 23 days ago)

Larger gauge needle ..... and I have heard that you can 'flex' the stem and take the biopsy diagonally and this helps you to get a nice core sample.

Check out these large, 16 gauge, 'core sampling' needles found at the local feed store .... $ .50 ... price was right.


--------------------


Extras: Filter Print Post Top
Offlineresptodd
I reject yourreality andsubstitute myown
Male User Gallery


Registered: 10/16/07
Posts: 674
Loc: Michigan
Last seen: 1 year, 10 months
Re: cloning [Re: uncle_rico]
    #7853054 - 01/09/08 10:56 AM (16 years, 23 days ago)

Uncle Rico, thanx to you also. I'm gonna head to the ag store right now to pick up some of those needles. Looks like that needle would be good for drawing up LC too. Hey shroober, good looking out, you are exactly correct.


--------------------
Damn! I'm having fun! Just keep the GD monkeys away.


Extras: Filter Print Post Top
OfflineRogerRabbitM
Bans for Pleasure
Male User Gallery


Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 3 days
Trusted Cultivator
OG Cultivator
Re: cloning [Re: uncle_rico]
    #7853058 - 01/09/08 10:57 AM (16 years, 23 days ago)

In front of a laminar flow hood, or inside a glovebox, simply shave a small piece of tissue from the inside of the stem and place on agar. Within 24 to 48 hours, you'll have a culture. If there's any contaminants, you'll easily see them on the petri dish, and can transfer healthy mycelium away from it to a fresh dish. When the culture is pure, transfer to grains.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


Extras: Filter Print Post Top
Invisibleuncle_rico
my own worst enemy
Male User Gallery

Registered: 03/28/06
Posts: 2,664
Re: cloning [Re: resptodd]
    #7853093 - 01/09/08 11:06 AM (16 years, 23 days ago)

Yes MAN ....

Long needles are good for sucking up LC etc.....

A couple of things I noticed:

My LC jar had holes that were sealed with silicone .... original hole was too small. So ..... increase the diameter of the holes ... obvious

When inoculating WBS with LC through a polyfill stuffed larger hole (polyfill was stuffed pretty tightly), the polyfill was pushed into the jar by the larger gauge needle. So don't pack the polyfill as tightly or have another method of gas exchange in your spawn jar lids.

Don't be retarded like me .... make accommodations for the larger diameter needle.

Good luck.


--------------------


Extras: Filter Print Post Top
Offlineresptodd
I reject yourreality andsubstitute myown
Male User Gallery


Registered: 10/16/07
Posts: 674
Loc: Michigan
Last seen: 1 year, 10 months
Re: cloning [Re: RogerRabbit]
    #7853099 - 01/09/08 11:07 AM (16 years, 23 days ago)

RR, I do have some lab malt agar petris. Good?---But I do want to make a LC. Can I transfer agar to LC?


--------------------
Damn! I'm having fun! Just keep the GD monkeys away.


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies   PhytoExtractum Kratom Powder for Sale, Maeng Da Thai Kratom Leaf Powder   Bridgetown Botanicals CBD Concentrates


Similar ThreadsPosterViewsRepliesLast post
* g2g transfer with polyfill-filled holes? merlink 972 1 08/04/03 11:16 AM
by dog
* grain transfers and spore printing esin 1,283 1 05/22/02 06:12 AM
by angryshroom
* transferring mycelium wikedanjel 4,113 13 01/10/03 03:54 AM
by debianlinux
* transferring "normal" to "peroxide agar" - dunk the tissue in h2o2 ? ohmatic 2,855 12 05/25/05 02:19 PM
by ohmatic
* Spores, G2G, Liquid Culture, Cloning & Colonization Time.
( 1 2 3 all )
agar 52,763 43 07/08/23 07:04 AM
by Screwup
* Mushroom cloning?? dimitri211 3,945 3 05/23/01 07:49 PM
by Anno
* PCing with polyfill jars DoDo 3,451 15 01/19/03 05:49 AM
by debianlinux
* innoculating polyfill jars Visigoth 2,364 1 01/05/02 10:42 PM
by Workman

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
698 topic views. 29 members, 362 guests and 81 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.022 seconds spending 0.007 seconds on 14 queries.