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Offlinecrumblebum
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Registered: 04/24/07
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Testing for contams before innoc.?
    #7844422 - 01/07/08 04:34 PM (16 years, 25 days ago)

I'm sure someone has thought of and asked about this before, but I really couldn't think of what to search for.

I assume that, after PCing PF Tek jars, assuming I don't end up transfering anything via needle during the innoc., any contams that are going to crop up are already in the jar. So would it be beneficial to let my jars sit at room temp for a week or two after PCing, to see if any of them are going to present contams before I waste spore solution on them?


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Invisibleorchidfanatic
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Re: Testing for contams before innoc.? [Re: crumblebum]
    #7844440 - 01/07/08 04:38 PM (16 years, 25 days ago)

yeah you can do that .. you can also leave one jar alone as a control and knock up all the others so you know if its technique or contams in the jars not killed by PC .. you can also put one or two drops of spore solution on agar to see if the syringe is contaminated.


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Offlinecrumblebum
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Re: Testing for contams before innoc.? [Re: orchidfanatic]
    #7844485 - 01/07/08 04:50 PM (16 years, 25 days ago)

Cool, thanks for the info.


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Invisibletahoe
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Re: Testing for contams before innoc.? [Re: crumblebum]
    #7844654 - 01/07/08 05:25 PM (16 years, 25 days ago)

its a good question and a good point. Once you get good at mycology you will be able to tell where your contamination was introduced.
cakes are easy. If its a straight line, usually dirty needle.
If it contams everywhere that the liquid was, dirty syringe.
if it contams elsewhere, bad sterilation technique.
as for grain jars, you usually shake before the contams get going so its like you shake it and 2 days later the whole jar is a lump of mold.

You can also use teh same pricipals to check grain jars.
You inoculate and do not shake for at least a week. Watch the mycelium and see what happens


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Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


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Offlinecrumblebum
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Re: Testing for contams before innoc.? [Re: tahoe]
    #7844759 - 01/07/08 05:44 PM (16 years, 25 days ago)

Awesome. Mostly I'm sticking with PF Tek jars because I understand the process well and it works, but I'll move up to grain in time. Right now I'm focusing on maximizing sterility, researching how to make a good glove box, and etc. This info will save me some heartbreak, and I'm pretty sure I can keep the needles clean, and syringes come from reputable vendor.


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InvisibleCrasher
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Re: Testing for contams before innoc.? [Re: crumblebum]
    #7844766 - 01/07/08 05:47 PM (16 years, 25 days ago)

Good luck.


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InvisibleDontPlay
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Re: Testing for contams before innoc.? [Re: crumblebum]
    #7844770 - 01/07/08 05:47 PM (16 years, 25 days ago)

You don't have to wait weeks before inoculation just a few days in the incubator and molds and most bacterias will present themselves I believe. I am a beginner so if im wrong please correct me.


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Invisibletahoe
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Re: Testing for contams before innoc.? [Re: crumblebum]
    #7844973 - 01/07/08 06:34 PM (16 years, 25 days ago)

Quote:

crumblebum said:
Awesome. Mostly I'm sticking with PF Tek jars because I understand



You have no idea How impressed i am with you. Very wise man. Master that pf tech, learn exactly what is going on at all times. Then go to grain.
I bet you have good success your first time since you have the mental capacity to know what is right from wrong


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Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread


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OfflineSubstance D
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Re: Testing for contams before innoc.? [Re: DontPlay]
    #7844975 - 01/07/08 06:34 PM (16 years, 25 days ago)

how long can his jars sit before he knocks them up after he pc them,just wondering if it would throw of the moisture .


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Invisibletahoe
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Re: Testing for contams before innoc.? [Re: Substance D]
    #7844999 - 01/07/08 06:38 PM (16 years, 25 days ago)

they can go months if they are sterile, maybe kept in the fridge, and kept air tight.

Back in the pf teck days I would lose track of which jars taht I had inoculated and after a month of colonization I would find a sterile jar with no growth


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread


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OfflineNibin
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Re: Testing for contams before innoc.? [Re: tahoe]
    #7846417 - 01/07/08 11:20 PM (16 years, 25 days ago)

I have kept boiled (not pc'd) jars covered in clingfilm so they didn't dry out for two weeks before inoculation.


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OfflineanarchOi
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Re: Testing for contams before innoc.? [Re: Nibin]
    #7846497 - 01/07/08 11:41 PM (16 years, 25 days ago)

seems to me like beginners usually have trouble with contams relating to too much water

it's damn hard to contam a PF tek during inoculation
and like was mentioned, an agar plate will easily test for a dirty syringe

cheers
good luck crum


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Offlineveda_sticks
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Re: Testing for contams before innoc.? [Re: anarchOi]
    #7846775 - 01/08/08 03:03 AM (16 years, 24 days ago)

If a jar has been sterilised properly and the dry verm filter is dry. I only see contamination coming from.

Dirty incoculation (from not using glovebox, laminar flow hood)
Dirty inoculant.

I prefer to inoculate as soon as the jars have cooled down, to give mycelium the best chance of survival. I have heard a few times that even pressure cooked jars are not guarenteed to be 100% sterile.

But i do see how it could be benificial for new people,


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Invisibleorchidfanatic
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Re: Testing for contams before innoc.? [Re: crumblebum]
    #7846982 - 01/08/08 07:09 AM (16 years, 24 days ago)

Quote:

crumblebum said:
A researching how to make a good glove box, and etc. This info will save me some heartbreak, and I'm pretty sure I can keep the needles clean, and syringes come from reputable vendor.




just get a clear tote box cut some holes for your arms on the side and
if you have extra money get a plexiglass plate to install on the lid so you can see better. then you wear latex gloves and spray the inside with lysol and wipe your gloved hands with alcohol gel hand sterilizer
I have done mycology with almost no contams using this still air box I just described. very simple ..
good luck !


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