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OfflineCallampin
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Registered: 08/14/03
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cloning question
    #7837130 - 01/05/08 08:13 PM (16 years, 27 days ago)

i havent been able to get the agar i needed and my flush is already harvested and its still going to be 2 more days till i get the agar,
so im wondering how long will the shroom i want to clone will last inside the fridge


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Offlinexhooliganx
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Re: cloning question [Re: Callampin]
    #7837152 - 01/05/08 08:25 PM (16 years, 27 days ago)

I've stored mushrooms for a few days in a cupboard before cloning and it was successful. make sure you swab it with iodine to clean it up before you do your agar work.


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OfflineCallampin
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Re: cloning question [Re: xhooliganx]
    #7837227 - 01/05/08 08:55 PM (16 years, 27 days ago)

nice , thanx


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InvisibleKillerPicklez
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Re: cloning question [Re: Callampin]
    #7837233 - 01/05/08 08:59 PM (16 years, 27 days ago)

You can always use Creamcorns brillant cloning tek, no agar required. Works excellent.

http://www.shroomery.org/forums/showflat.php?Cat=0&Board=2&Number=5874305&fpart=&PHPSESSID=


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Offlinexhooliganx
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Re: cloning question [Re: KillerPicklez]
    #7837298 - 01/05/08 09:18 PM (16 years, 27 days ago)

yeah there's always lc cloning or I like doing clone to grain every once in a while. but cloning to agar allows you to make sure your clone is clean and also transfer to a slant for specimen preservation


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Offlineveda_sticks
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Re: cloning question [Re: xhooliganx]
    #7837652 - 01/05/08 10:49 PM (16 years, 27 days ago)

Have never attempted a clone yet, i have kept fresh mushrooms for upto 5 days in the fridge.

However the longer you keep them the more exposed they get to contaminants, its better to clone them when very fresh.

I know RR has great success soaking tissue in a bleach solution before cloning.

The cleanest upi get the tissue the better, the less transfers, resulting in a younger generation of myc (thats the way i see it anyways)


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PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek


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Offlinexhooliganx
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Re: cloning question [Re: veda_sticks]
    #7837779 - 01/05/08 11:30 PM (16 years, 27 days ago)

Quote:

veda_sticks said:
Have never attempted a clone yet, i have kept fresh mushrooms for upto 5 days in the fridge.

However the longer you keep them the more exposed they get to contaminants, its better to clone them when very fresh.

I know RR has great success soaking tissue in a bleach solution before cloning.

The cleanest upi get the tissue the better, the less transfers, resulting in a younger generation of myc (thats the way i see it anyways)



I didn't ever see a post where rr ever suggested cleaning tissue with bleach. only iodine. just tried searching with no avail. can you find a link for us?


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Offlineveda_sticks
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Re: cloning question [Re: xhooliganx]
    #7838088 - 01/06/08 02:19 AM (16 years, 27 days ago)

I dont know what topic he mentioned it, it wasnt directly related to the thread but it popped up.

I could be getting my wires complelty crossed however.


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek


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OfflineNibin
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Re: cloning question [Re: xhooliganx]
    #7838135 - 01/06/08 02:49 AM (16 years, 27 days ago)

Quote:

xhooliganx said:
Quote:

veda_sticks said:
Have never attempted a clone yet, i have kept fresh mushrooms for upto 5 days in the fridge.

However the longer you keep them the more exposed they get to contaminants, its better to clone them when very fresh.

I know RR has great success soaking tissue in a bleach solution before cloning.

The cleanest upi get the tissue the better, the less transfers, resulting in a younger generation of myc (thats the way i see it anyways)



I didn't ever see a post where rr ever suggested cleaning tissue with bleach. only iodine. just tried searching with no avail. can you find a link for us?




Using search engine, I typed in Clone Bleach, and username RogerRabbit.

The first post the search turned up was this
http://www.shroomery.org/forums/showflat.php/Number/5925703#5925703


Quote:

regularly dip cultures from the wild into ten percent bleach before transferring to agar to kill mold spores and bacteria, but it doesn't harm the live culture. That's why spraying bleach on the black mold around your bathtub or window sills does nothing.

Iodine is very effective against microbes. When I clone, I wash the whole mushroom with iodine before cutting out a section to transfer, which is then dipped into ten percent bleach.

Peroxide will help prevent mold in your humidifiers. Many experienced mycologists also use peroxide to dip cultures before transferring to agar. I prefer not to use it for that purpose except for when transferring dry tissue because of the damage it causes at the cellular level, which the fungi must then recover from.




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