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InvisibleBridgeburner
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is my TiT contaminated?
    #7834395 - 01/05/08 02:35 AM (16 years, 27 days ago)

this is driving me nuts, i've wasted don't know how many jars on it already.

anyway all my jars got contams a while back: all the same ones. so i thought it wasn't the inoculation problem or sterilization problem because they were done at different times with different tools.

so i made totally new jars and followed harsher sterilization processes. still today i took a whiff of the jars and with little myc growth i smell the contams.

so all i can think of is that the TiT has contam spores in it and they infect the jars. is this possible?


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Invisibleflavoraid
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Re: is my TiT contaminated? [Re: Bridgeburner]
    #7834401 - 01/05/08 02:48 AM (16 years, 27 days ago)

doubt it, and if in doubt swab the crap out of it with isopropal or put it outside and put a big bottle of bleach in there with water put an aquarium bubbler in there too just to agitate everything.


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coda said:
imachavel, Man you really need to do some reading, the amount of bullshit you put into almost every single one of your posts is absolutely astounding.


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Invisibleshaggydogman
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Re: is my TiT contaminated? [Re: Bridgeburner]
    #7834417 - 01/05/08 02:58 AM (16 years, 27 days ago)

Someone gave advice earlier on in another thread about creating a control jar with each batch. This sounds like a very good idea in your case. Make a batch of jars, sterilize etc, then inoculate all except one, which will be your control jar. Then incubate them all.

If your control gets a contam then it's probably a sterilization problem or your TiT
If your control jar doesn't get contam them it's your inoculant or inoculation procedures.

Give your TiT a good clean out with soap and water, maybe a bleach wipe as well.
How did you sterilize the jars? (steam or PC) How long?
How warm is your TiT? Higher temps will encourage bacterial growth.


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Rye -- WBS | Grain LC -- G2G | Bulk -- Monotub | 50/50 -- Late Casing -- A Pinning Strategy
Disclaimer: My opinion is subject to change at short notice subject to but not limited by new information and knowledge being made available.


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InvisibleCrasher
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Re: is my TiT contaminated? [Re: flavoraid]
    #7834420 - 01/05/08 02:58 AM (16 years, 27 days ago)

I would spray Lysol on the inside and add h2o2 to your water if you've got that running for heat.


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Give me silence, water, hope;
Give me struggle, iron, volcanoes...


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InvisibleBridgeburner
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Re: is my TiT contaminated? [Re: Crasher]
    #7834428 - 01/05/08 03:04 AM (16 years, 27 days ago)

steamed them for an hour & half.

i'll wipe the tub down with alcohol.


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Invisibleshaggydogman
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Re: is my TiT contaminated? [Re: Bridgeburner]
    #7834430 - 01/05/08 03:06 AM (16 years, 27 days ago)

Hour and a half sounds like you should be OK there. A control on your next batch will prove it. :smile:

What are you using to inoculate them with?


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Rye -- WBS | Grain LC -- G2G | Bulk -- Monotub | 50/50 -- Late Casing -- A Pinning Strategy
Disclaimer: My opinion is subject to change at short notice subject to but not limited by new information and knowledge being made available.


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InvisibleCrasher
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Re: is my TiT contaminated? [Re: Bridgeburner]
    #7834431 - 01/05/08 03:08 AM (16 years, 27 days ago)

also, clean up the area surrounding your TiT.

I've read a few recommendations that say you shouldn't have your operation at ground level either. I don't know your circumstances.


--------------------
Give me silence, water, hope;
Give me struggle, iron, volcanoes...


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InvisibleBridgeburner
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Re: is my TiT contaminated? [Re: Crasher]
    #7834434 - 01/05/08 03:11 AM (16 years, 27 days ago)

the last grow i did i had zero contams. i didn't have an incubation chamber and i needed one as my room temps are low. anyway i used the fc as a heated environment and that kind of worked.

i used multispore.

i'm really stumped on this one. i made a few jars that i plan to fruit in vitro but i won't put them into that TiT. damn, it's a real bummer.


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Invisibleshaggydogman
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Re: is my TiT contaminated? [Re: Bridgeburner]
    #7834441 - 01/05/08 03:14 AM (16 years, 27 days ago)

I feel your pain....been there myself!!

Is the contam bacterial?
How warm is the Tit?


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Rye -- WBS | Grain LC -- G2G | Bulk -- Monotub | 50/50 -- Late Casing -- A Pinning Strategy
Disclaimer: My opinion is subject to change at short notice subject to but not limited by new information and knowledge being made available.


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InvisibleRoadkillM
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Re: is my TiT contaminated? [Re: Bridgeburner]
    #7834442 - 01/05/08 03:15 AM (16 years, 27 days ago)

Quote:

b0red5tiff said:

steamed them for an hour & half.






steamed what for 1 1/2 hours?

pf (brf/verm) jars or grain jars?


what tek are you following?


are you using Vendor syringes or did you make your own?


odds are that your syringes or substrate is probably bad...
very unlikely that your TIT incubator is contaminated and causing you problems.


tc


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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Invisibleshaggydogman
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Re: is my TiT contaminated? [Re: shaggydogman]
    #7834447 - 01/05/08 03:19 AM (16 years, 27 days ago)

So here's a test to find out where the problem is...

Make and sterilise 4 jars
inoculate 2, leave 2 as controls.
Put 1 control and 1 inoculated into the tit
leave other 2 at room temperature.

The results will give you all the info you need to work out what is going on.


Edit: Good point roadkill, this is all assuming they are pf tek brf/verm jars...


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Rye -- WBS | Grain LC -- G2G | Bulk -- Monotub | 50/50 -- Late Casing -- A Pinning Strategy
Disclaimer: My opinion is subject to change at short notice subject to but not limited by new information and knowledge being made available.


Edited by shaggydogman (01/05/08 03:21 AM)


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InvisibleBridgeburner
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Re: is my TiT contaminated? [Re: shaggydogman]
    #7834452 - 01/05/08 03:22 AM (16 years, 27 days ago)

it's not mold, just a thick clump always in the middle of jars that smells really really foul & sour, you don't have to know jackshit about mycology to know that's just not right...

i did the usual PF-TEK brf jars.

the TiT is 26C-27C.

used both vendor spore prints & home made ones. never had a problem with either of them.

i'll just have to use another way to provide heat to the new jars.


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Invisibleshaggydogman
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Re: is my TiT contaminated? [Re: Bridgeburner]
    #7834460 - 01/05/08 03:30 AM (16 years, 27 days ago)

That'll be bacterial then. Being in the middle of the jar rules out the innoculant.

Being in the middle would suggest the sterilisation of the jars as the problem. With the arguably high temp in the tit compounding the problem.

Up the time you steam them for. Lower the incubation temps a few degrees.
Even better, invest in a PC.

p.s. Are they half pint jars?


Edited by shaggydogman (01/05/08 03:35 AM)


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InvisibleBridgeburner
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Re: is my TiT contaminated? [Re: shaggydogman]
    #7834466 - 01/05/08 03:33 AM (16 years, 27 days ago)

no pc's for sale here.


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Invisibleshaggydogman
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Re: is my TiT contaminated? [Re: Bridgeburner]
    #7834468 - 01/05/08 03:36 AM (16 years, 27 days ago)

No PC's...bummer...

What size are the jars?
What altitude are you at?


--------------------
Rye -- WBS | Grain LC -- G2G | Bulk -- Monotub | 50/50 -- Late Casing -- A Pinning Strategy
Disclaimer: My opinion is subject to change at short notice subject to but not limited by new information and knowledge being made available.


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InvisibleBridgeburner
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Re: is my TiT contaminated? [Re: shaggydogman]
    #7834470 - 01/05/08 03:38 AM (16 years, 27 days ago)

200ml-350ml.

don't know the altitude, how's that gonna affect the grow?

i don't think steaming has got anything to do with this, worked fine before.


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Invisibleshaggydogman
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Re: is my TiT contaminated? [Re: Bridgeburner]
    #7834477 - 01/05/08 03:43 AM (16 years, 27 days ago)

Water boils at a lower temperature the higher you are. Therefore less heat to sterilize with
http://www.chemistry.co.nz/cooking_altitudes.htm

Was just checking you didn't have pint jars.

I'm pretty sure from how you describe it that your not getting the inside of the jars hot enough during the steam, so a longer steam will help. If you didn't previously incubate the issue may not have come up, but the extra temperature you are giving them by incubating them is encouraging the bacterial growth in the middle of the jars.

So I think, steam for 2 hours, reduce Tit temperature to 25C max and try the 4 jar test from above so you get enough info to work out what is going on.



--------------------
Rye -- WBS | Grain LC -- G2G | Bulk -- Monotub | 50/50 -- Late Casing -- A Pinning Strategy
Disclaimer: My opinion is subject to change at short notice subject to but not limited by new information and knowledge being made available.


Edited by shaggydogman (01/05/08 03:46 AM)


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InvisibleBridgeburner
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Re: is my TiT contaminated? [Re: shaggydogman]
    #7834497 - 01/05/08 04:33 AM (16 years, 27 days ago)

i find that hard to believe, like i said it always worked before, no reason why it shouldn't now.


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Invisibleshaggydogman
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Re: is my TiT contaminated? [Re: Bridgeburner]
    #7834500 - 01/05/08 04:44 AM (16 years, 27 days ago)

When it worked before what temperature were the jars left in to colonise?


--------------------
Rye -- WBS | Grain LC -- G2G | Bulk -- Monotub | 50/50 -- Late Casing -- A Pinning Strategy
Disclaimer: My opinion is subject to change at short notice subject to but not limited by new information and knowledge being made available.


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OfflineNibin
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Re: is my TiT contaminated? [Re: Bridgeburner]
    #7834501 - 01/05/08 04:45 AM (16 years, 27 days ago)

Have you changed your spore supplier?

Also, try and make a batch unincubated. Incubation will speed up not only cube mycelium growth but also contaminant growth (which happens to grow faster than cube mycelium) and the temperature boost gives the contaminants an edge over the mycelium.


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