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RoYaL_fLuSh
Stranger


Registered: 01/04/08
Posts: 499
Loc: UK
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over inoculation ?
#7834193 - 01/05/08 12:14 AM (16 years, 28 days ago) |
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ok for my second stupid question ... what would happen if i accidentially inoculated a brf jar with 2 cc of syringe solution instead of 1 cc ... say of .. treasure coast spores , ... for speculations sake ..
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BUDDHA_702
Master Mycologist In Training



Registered: 02/17/07
Posts: 1,296
Loc: Some Country
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worst that would happen is that you would be short a CC or spores. One extra CC is'nt to bad, 2-4 & you should toss it. IMO
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JewelessCaesar
Shroomery's Legal Department




Registered: 05/26/06
Posts: 599
Loc: Petros-Joyner, TN, USA
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Re: over inoculation ? [Re: BUDDHA_702]
#7834373 - 01/05/08 02:04 AM (16 years, 28 days ago) |
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Quote:
BUDDHA_702 said: worst that would happen is that you would be short a CC or spores. One extra CC isn't to bad, 2-4 & you should toss it. IMO
Sorry but you contradicted yourself. You say worst that would happen would be a wasted amount of spores then tell him to toss a 2cc. The real danger in over inoculation is changing your substrates water ratio. If your sub was on the drier end of the scale you'll definitely be fine. But none the less keep us updated and good luck again my friend. Oh yeah I also find that I can use between .5 to 1 cc by angling the syringe to the outside of the jar and squirting it onto the glass and letting the spores slide down the side and lettings them have a chance to run as far as they can go on their own accord, then I give them a good shake for better distribution and then I play the hurry up and wait game!
JCLE
-------------------- Failure is not an option, it's an adventure! - JewelessCaesar
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flavoraid
now with twicethe ketamine andopiates!


Registered: 12/05/07
Posts: 1,678
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yeah the damage is done by having too much moisture in your jars.. I know people who have made 10 syringes from 4 prints and they were BLACK it's not the spores that cause harm. I don't think you can have too many spores at a certain point more spores won't help though.
-------------------- coda said: imachavel, Man you really need to do some reading, the amount of bullshit you put into almost every single one of your posts is absolutely astounding.
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RoYaL_fLuSh
Stranger


Registered: 01/04/08
Posts: 499
Loc: UK
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Re: over inoculation ? [Re: flavoraid]
#7834435 - 01/05/08 03:11 AM (16 years, 28 days ago) |
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Quote:
flavoraid said: yeah the damage is done by having too much moisture in your jars.. I know people who have made 10 syringes from 4 prints and they were BLACK it's not the spores that cause harm. I don't think you can have too many spores at a certain point more spores won't help though.
yeah moisture was my concern .. perhaps a low level humidity area would help givin such a case .. you think ?
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Roadkill
Retired Shroomery Mod



Registered: 12/11/01
Posts: 22,674
Loc: Montana
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Quote:
RoYaL_fLuSh said:
what would happen if i accidentially inoculated a brf jar with 2 cc of syringe solution instead of 1 cc
I use 2 cc per jar in my pf tek(brf/verm) jars...
it works just fine.
tc
-------------------- Laterz, Road Who the hell you callin crazy? You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch! Brainiac said: PM the names with on there names, that means they have mushrooms for sale.
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RoYaL_fLuSh
Stranger


Registered: 01/04/08
Posts: 499
Loc: UK
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Re: over inoculation ? [Re: Roadkill]
#7834451 - 01/05/08 03:21 AM (16 years, 28 days ago) |
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Quote:
Roadkill said:
Quote:
RoYaL_fLuSh said:
what would happen if i accidentially inoculated a brf jar with 2 cc of syringe solution instead of 1 cc
I use 2 cc per jar in my pf tek(brf/verm) jars...
it works just fine.
tc
ty worries have subsided haha
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Nibin
Getting there



Registered: 11/29/05
Posts: 4,480
Last seen: 10 years, 8 months
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Re: over inoculation ? [Re: flavoraid]
#7834508 - 01/05/08 04:56 AM (16 years, 27 days ago) |
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Quote:
flavoraid said: yeah the damage is done by having too much moisture in your jars.. I know people who have made 10 syringes from 4 prints and they were BLACK it's not the spores that cause harm. I don't think you can have too many spores at a certain point more spores won't help though.
The number of spores can be a big issue, bigger than one extra cc of water, especially if your syringes are very dark. Too many spores produce lots of competing substrains, some of which are not compatible. So instead of ending with a single mycelial network in your cake, you end up with a few, each fruiting independently and flushes will be smaller (as each individual network has access to less nutrients and water)
Quote:
RogerRabbit said: Some growers have reported very fast 'colonization' with extremely full syringes. Healthy, fresh spores will begin to germinate within 20 minutes of being placed into grains or agar.
What hasn't ever been reported is nice flushes from very dark syringes. Any time you have thousands of substrains, at least a few will be non-compatible and won't merge into a single organism, which inhibits fruiting.
In addition, since no print is completely sterile, the more spores, the more contaminants that go along for the ride, thus often the contamination rates are higher.
RR
But in your case I'm pretty sure you will be fine.
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