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resptodd
I reject yourreality andsubstitute myown



Registered: 10/16/07
Posts: 674
Loc: Michigan
Last seen: 1 year, 10 months
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Making a spore syringe
#7832527 - 01/04/08 04:14 PM (16 years, 28 days ago) |
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Well I'm planning on making a spore syringe. I have access to sterile medical equipt. and supplies. Here's what I'm thinking of trying. I have a sterile specimen cup(kind used for urine tests)-if I place a shroom cap inside the cup while it's dropping spores, remove, inject cup with sterile water then suck up into a syringe---spore syringe? my question is this--placing the mush cap into the cup, could the mush cap itself transfer contams into the cup? I plan on using a sterile tek all the way, including using sterile surgical gloves to pick and cut the cap off. If I don't inject cup with water is this a good way to store the spores?
-------------------- Damn! I'm having fun! Just keep the GD monkeys away.
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ShroomieGirl
What are these god damn animals



Registered: 05/29/07
Posts: 1,938
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Re: Making a spore syringe [Re: resptodd]
#7832612 - 01/04/08 04:37 PM (16 years, 28 days ago) |
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it could, but assuming your fc is sterile (or as sterile as it can be), there shouldnt be any trouble esp if youre using a glovebox.
You can store it as a spore print... just fold up the aluminum you let the spores drop on, and put it into the fridge. Takes up less space that way too.
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veda_sticks
Cultivator




Registered: 07/29/07
Posts: 14,191
Loc: UK
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Yes that method works, theer is actually a tek that describes using a half pint pf jar to take the print, put the lid in, then simply inject and suck back up the water.
I would think that laying a print with the glovebox, but transering the cap after 4 hours to a new peice of foil will produce a cleaner print.
Also not that its not nessesary to make dark syringes, the more of a print u use the more chance of contaminants to go along for the ride. Also more spores means more genetic varience.
-------------------- PF TEK - writeup by EvilMushroom666 Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD Cakes can and will pin! - So you think cakes suck for pins. Your wrong Franks Simple Coir/Verm Tek Franks Proper Pasturisation Tek Franks Spawning To Bulk - Monotub Professor Pinheads RTV Injection Port Tek Foo Mans No Soak WBS Prep Tek
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Nature Boy
Stranger than most


Registered: 07/09/07
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Loc: Samsara
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Re: Making a spore syringe [Re: resptodd]
#7832861 - 01/04/08 05:46 PM (16 years, 28 days ago) |
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Here's how I've done it. Since I posted this, I came to learn that others had done it in a fairly similar way before...I just independently "discovered" this method. I find it still works great.
http://www.shroomery.org/forums/showflat.php/Cat/0/Number/7335688/an/0/page/0
To sterilize the jar, wire, and lid, place in the oven on "bake" at 300 degrees for 20 minutes. Even a toaster oven will do. DO NOT open the door abruptly to hasten the cooling or you may crack the glass.
If using a full sized oven, you can crack the door an inch or so to allow heat to escape. SUSPEND the cap (use a small to medium sized cap) about 1 cm above the bottom of the jar and leave it overnight. You'll have PLENTY of spores for your syringe.
Regards, N.B.
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resptodd
I reject yourreality andsubstitute myown



Registered: 10/16/07
Posts: 674
Loc: Michigan
Last seen: 1 year, 10 months
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Re: Making a spore syringe [Re: veda_sticks]
#7833026 - 01/04/08 06:36 PM (16 years, 28 days ago) |
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Veda sticks---what do you mean by the 4hrs thing? I have a thick skull I don't always understand things right off.
-------------------- Damn! I'm having fun! Just keep the GD monkeys away.
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Nibin
Getting there



Registered: 11/29/05
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Re: Making a spore syringe [Re: resptodd]
#7834551 - 01/05/08 05:46 AM (16 years, 27 days ago) |
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Quote:
resptodd said: Veda sticks---what do you mean by the 4hrs thing? I have a thick skull I don't always understand things right off.
The chances are that the first spores dropped contain more contaminant spores due to the unsterile environment of the FC. So you let the first 4 hours worth of spores drop and you toss em, then transfer the cap to another piece of foil and let those spores drop. Hopefully those spores will be cleaner than the previous ones.
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veda_sticks
Cultivator




Registered: 07/29/07
Posts: 14,191
Loc: UK
Last seen: 4 years, 25 days
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Re: Making a spore syringe [Re: Nibin]
#7834614 - 01/05/08 06:41 AM (16 years, 27 days ago) |
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exactly, thanks nibin
-------------------- PF TEK - writeup by EvilMushroom666 Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD Cakes can and will pin! - So you think cakes suck for pins. Your wrong Franks Simple Coir/Verm Tek Franks Proper Pasturisation Tek Franks Spawning To Bulk - Monotub Professor Pinheads RTV Injection Port Tek Foo Mans No Soak WBS Prep Tek
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