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Invisibleshroomiin
unprofound

Registered: 12/24/07
Posts: 2,470
Loc: Zone 6 Flag
Confused about inoculation...
    #7829870 - 01/03/08 10:05 PM (16 years, 29 days ago)

Okay so this is my first attempt at cultivating some mushrooms and i am a little confused. After inoculation, and i tape over all of the holes what temperature should the jars be stored in? I have an incubator but the temperature is a little over 86* (~90), would that hurt? Or should i just leave the jars in room temperature? (~68-70*)

Thanks...


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InvisibleRobo
R Series 66Y
Registered: 05/08/07
Posts: 14,861
Re: Confused about inoculation... [Re: shroomiin]
    #7829894 - 01/03/08 10:09 PM (16 years, 29 days ago)

I've put them up on a shelf in a closet, they colonized just fine.


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OfflineHappyTripping
Tranceformer
Registered: 04/16/06
Posts: 119
Last seen: 14 years, 8 months
Re: Confused about inoculation... [Re: shroomiin]
    #7829903 - 01/03/08 10:11 PM (16 years, 29 days ago)

Room temperature is just fine. It is preferable to 86 in an incubator -- that's just too hot for optimum mycelium growth and invites bacterial infection. Anything in the 70s will suit you fine, but 68-70 is perfectly acceptable.


--------------------
(Everything written here is the work or genesis of my best buddy's girlfriend's dog's-friend's cat's owner. If it has been written in the first person, the reason has been for clarity.)


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Invisiblethedefone
deus ex machina

Registered: 10/06/07
Posts: 1,883
Loc: Gondwana
Re: Confused about inoculation... [Re: HappyTripping]
    #7829941 - 01/03/08 10:18 PM (16 years, 29 days ago)

Room temperature works just fine, and is probably better, because if you are incubating your jars, you are also incubating any possible contaminates. If you do incubate, nearer to 81 is what you are shooting for, because the jars themselves will put out a couple degrees of heat. So, you should lower that a little bit.

Incidentally, If you are doing a PFTek style grow, with a verm barrier, you can do without the tape on the holes. That is what the verm is there for. Remove any foil, or tape, or anything else impeding the exchange of gases form your jars, and they will thank you later.


--------------------


I am become death, the destroyer of worlds.


Edited by thedefone (01/03/08 10:19 PM)


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Offlineveda_sticks
Cultivator
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Registered: 07/29/07
Posts: 14,191
Loc: UK Flag
Last seen: 4 years, 25 days
Re: Confused about inoculation... [Re: HappyTripping]
    #7829962 - 01/03/08 10:21 PM (16 years, 29 days ago)

Here is a nice quote from RR

Wild swings in temperature cause air exchanges between the jars and the outside. If the changes are rapid, they can easily be too much for the filtering material, especially if only dry verm is used. I don't recommend incubators, but find a nice room that holds at least to within five degrees or so. 75F to 81F is ideal. Above that, the returns are not worth the increased rate of contamination that will be experienced.
RR

86 is not optimal since myc will produce its own heat.

If your using the pf tek, there is no need to tape the holes, the dry verm layer acts as a filter catching any unwanted contaminant spores, micropore tape can be used as an extra guard and will still provide the required gas exchange, also the tinfoil covered your jars to prevent moisture entering is not needed either.


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek


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Offlinebosr
money overbitches


Registered: 01/03/08
Posts: 24
Last seen: 16 years, 28 days
Re: Confused about inoculation... [Re: veda_sticks]
    #7829973 - 01/03/08 10:23 PM (16 years, 29 days ago)

no tape.
70-81 is best


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InvisibleRobo
R Series 66Y
Registered: 05/08/07
Posts: 14,861
Re: Confused about inoculation... [Re: bosr]
    #7829988 - 01/03/08 10:25 PM (16 years, 29 days ago)

The verm layer is ok but I still use micropore tape.


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Offlineveda_sticks
Cultivator
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Registered: 07/29/07
Posts: 14,191
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Last seen: 4 years, 25 days
Re: Confused about inoculation... [Re: Robo]
    #7830021 - 01/03/08 10:32 PM (16 years, 29 days ago)

Quote:

Omni said:
The verm layer is ok but I still use micropore tape.




Its a good method just as an extra precaution since its breathable, i have never used it as i cant be bothered going to get it, i have had great success with just the dry verm layer. It does its job well (when its kept dry)


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek


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OfflineBanjoMojo
Munster
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Registered: 09/29/07
Posts: 148
Loc: Alabama-ish
Last seen: 4 years, 4 months
Re: Confused about inoculation... [Re: veda_sticks]
    #7830221 - 01/03/08 11:12 PM (16 years, 29 days ago)

I noticed in some of my jars that as the mycelium is colonizing the substrate, the forming cake tends to pull back from the sides of the jar. This has caused the dry verm layer on top of my cake to become the dry verm layer on top and all around some of my cakes. Since this has caused my top verm layers to become patchy in places, I'm glad I used micropore tape over my inoculation holes.


--------------------
:firefox: If God is inside us like some people say, He'd better like burritos 'cause that's what he's getting. :firefox:

I ♥ :skittles:


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Offlineveda_sticks
Cultivator
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Folding@home Statistics
Registered: 07/29/07
Posts: 14,191
Loc: UK Flag
Last seen: 4 years, 25 days
Re: Confused about inoculation... [Re: BanjoMojo]
    #7830237 - 01/03/08 11:17 PM (16 years, 29 days ago)

yes that could be an advantage, this has happened in quite a few of my jars before 100% colinisation but has yet to be a problem, 1 of my current jars had dry verm drop down onto an uncolinised portion, which has now colinised.

Its not been a problem for me


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek


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Invisibletahoe
Noob Slayer
Male User Gallery


Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
Trusted Cultivator
Re: Confused about inoculation... [Re: bosr]
    #7830370 - 01/04/08 12:08 AM (16 years, 29 days ago)

Quote:

bosr said:
no tape.




No tape?
Nice gape!!!!


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread


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Invisiblefastfred
Old Hand
Male User Gallery

Folding@home Statistics
Registered: 05/17/04
Posts: 6,899
Loc: Dark side of the moon
Re: Confused about inoculation... [Re: thedefone]
    #7830671 - 01/04/08 03:41 AM (16 years, 28 days ago)

Quote:

thedefone said:
Incidentally, If you are doing a PFTek style grow, with a verm barrier, you can do without the tape on the holes. That is what the verm is there for. Remove any foil, or tape, or anything else impeding the exchange of gases form your jars, and they will thank you later.




Do not remove the foil cover. Covering everything with foil is standard lab practice. It is there to prevent contams from entering your culture. The verm barrier really does little or nothing to prevent contams. Any contam spores or bacteria falling onto the verm will receive plenty of moisture to germinate from respiration, condensation, and the amount it soaks up during sterilization. In many cases contams will simply fall right through the verm "barrier".


-FF


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Offlineveda_sticks
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Posts: 14,191
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Last seen: 4 years, 25 days
Re: Confused about inoculation... [Re: fastfred]
    #7830716 - 01/04/08 04:34 AM (16 years, 28 days ago)

that does make sense, but that conflicts what alot of other experienced growers have said, i have even heard of people being able to remove the lid without contamination (as long as the verm layer is not disturbed too much).

I have never had a problem leaving the inoc holes exposed.


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek


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Invisiblefastfred
Old Hand
Male User Gallery

Folding@home Statistics
Registered: 05/17/04
Posts: 6,899
Loc: Dark side of the moon
Re: Confused about inoculation... [Re: veda_sticks]
    #7830789 - 01/04/08 05:40 AM (16 years, 28 days ago)

If you want to rely on a quater inch of loosely-packed, semi-damp porous material to prevent dust and contams from getting to your substrate that's fine. It's cheaper and easier to use a piece of foil.

Personally I never used a verm barrier as it seemed like a real waste of space and materials to me. Any contams you inject will be right on your substrate anyways and if you have a foil cover no contams are going to drop onto your substrate. The only time the verm layer could be of any use is if a contam somehow got into your jar and landed on it, but failed to germinate.

Given that most people use foil anyways to prevent water from entering their jars during sterilization the verm layer is a complete waste of space and money.


-FF


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