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tahoe
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Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
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Pioneering New and Radical Cultivation Concepts update Failure!!
#7828563 - 01/03/08 06:04 PM (16 years, 29 days ago) |
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Okay, maybe not pioneering but I am trying something new.
I have some existing Shiitake plug/dowel spawn that I would like to use for an indoor shiitake log. I do not feel like putting these to agar and transferring to bran/sawdust so I was thinking of what I could do.
So here it is. I was about to layer some wbs/alder saw dust in a jar without cooking the grain first but then I realized that it would boil in the jar and the alder would not stay on top. So I boiled up 1/2 cup of wbs and put it in the pint jar

I took some of this

Mixed it with some water to get it a little wet.

Then I wiped down the sides of the jar sort of like the pf tek to keep any nutrients off the sides. I added the alder chips.


Your typical tyvek seal.

She is in there with the big boys. The biggins will be used for the lions mane and sra that I just got from www.sporeworks.com

So here is the rest of the plan. After it cools I will take one of these dowels and just lay it on top of the alder chips in the jar. My hopes are that the mycelium will colonize the alder/contam layer and then go to town on the wbs. Once fully colonized there will be enough spawn to know up a bag of wood chips. We shall see what happens
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
Edited by tahoe (01/20/08 02:40 PM)
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Culland
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Registered: 09/12/07
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Re: Pioneering New and Radical Cultivation Concepts [Re: tahoe]
#7828602 - 01/03/08 06:14 PM (16 years, 29 days ago) |
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Do you think the alder chips is necessary? I have been mucking around with a birch polypore culture and it leaps to grain a lot faster then to sawdust, assuming because of the higher nutrient content of grain.
Cul
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tahoe
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Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
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Re: Pioneering New and Radical Cultivation Concepts [Re: Culland]
#7828654 - 01/03/08 06:25 PM (16 years, 29 days ago) |
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what do you mean by necessary? For what I am doing, absolutely. For making spawn for shiitake logs, No.
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
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RogerRabbit
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Registered: 03/26/03
Posts: 42,214
Loc: Seattle
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Re: Pioneering New and Radical Cultivation Concepts [Re: tahoe]
#7829429 - 01/03/08 08:26 PM (16 years, 29 days ago) |
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I have some similar going with quart jars. I have a flowhood though, so sterilize alder sawdust/chips along with rice or wheat bran and gypsum in quart jars, and then later on inoculate with colonized rye. It works great to fruit right out of the top of the jar. I assume you're planning to use syringes, since you put the grains in there. You could also use brf or rice bran mixed with the sawdust. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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tahoe
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Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
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Re: Pioneering New and Radical Cultivation Concepts [Re: RogerRabbit]
#7830121 - 01/03/08 10:52 PM (16 years, 29 days ago) |
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Actually the plan is to just set one of those colonized plugs right on top of the alder chips. i am hoping that the plug will colonize the alder and then reach down to the grain without introducing contams.
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
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Culland
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Registered: 09/12/07
Posts: 324
Last seen: 17 days, 4 hours
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Re: Pioneering New and Radical Cultivation Concepts [Re: tahoe]
#7830833 - 01/04/08 06:29 AM (16 years, 28 days ago) |
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I understand more now. So the alder chips are just a filter layer to keep contams out of the wbs? Is this because the dowels are not necessarily sterile?
Cul
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tahoe
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Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
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Re: Pioneering New and Radical Cultivation Concepts [Re: Culland]
#7836971 - 01/05/08 07:20 PM (16 years, 27 days ago) |
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when dowels are done correctly they are sterile. This one has been in a bag in my fridge for years.
You are correct in your understanding
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
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tahoe
Noob Slayer



Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
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Re: Pioneering New and Radical Cultivation Concepts [Re: tahoe]
#7906035 - 01/20/08 02:45 PM (16 years, 12 days ago) |
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shortly after I started this project I realized that the wood chip barrier would dry out and would need moisture. This is were the problem lies. Adding water to just the chips without introducing contams isnt impossible but not worth the extra effort. I just sprayed it with my misting bottle and some blue mold started to form last week. I suppose I could have used a more air tight lid and this would not have been a problem. I used a tyvek lid and it just allowed too much moisture out. I could have just used a plastic lid and I think it would have been fine even though co2 levels might have been high I would not have made a big deal about it
Next projects, sra, blue oyster, lions mane-which is fruiting on agar as we speak
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
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