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Invisiblekupa trooper
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question on liquid culture?
    #7827502 - 01/03/08 01:34 PM (16 years, 29 days ago)

when you make a liquid culture from multispore are there different mycs growing in the jar or does the dominant spore take over


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I don't know but I've been told. It's hard to run with the weight of gold                                                                  On the other hand I heard it said. It's just as hard with the weight of lead.


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OfflineBanjoMojo
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Re: question on liquid culture? [Re: kupa trooper]
    #7827565 - 01/03/08 01:50 PM (16 years, 29 days ago)

From my understanding, whenever you do multispore, when the spore's individual strands of mycelium meet, they in essence, create a new strain. If all of your spores are from the same strain, which is generally the case, it isn't a huge problem, but you run the risk of having a trait that is more abundantly represented presenting itself in the final fruits.

There are some good threads on creating isolates and master slants in Advanced Mycology that are always a good read on some more complex methods.


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Edited by BanjoMojo (01/03/08 01:51 PM)


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Invisiblekupa trooper
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Re: question on liquid culture? [Re: BanjoMojo]
    #7827884 - 01/03/08 03:05 PM (16 years, 29 days ago)

this is not exactly bad? is it possible that it my produce mutant or other strange variations in final fruits?


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I don't know but I've been told. It's hard to run with the weight of gold                                                                  On the other hand I heard it said. It's just as hard with the weight of lead.


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OfflineBanjoMojo
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Re: question on liquid culture? [Re: kupa trooper]
    #7828591 - 01/03/08 06:10 PM (16 years, 29 days ago)

That's always a possibility, but when you are dealing with hundreds of thousands of spores, it usually isn't an issue unless they come from a genetically unsound fruit.


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:firefox: If God is inside us like some people say, He'd better like burritos 'cause that's what he's getting. :firefox:

I ♥ :skittles:


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Offlineveda_sticks
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Re: question on liquid culture? [Re: BanjoMojo]
    #7829695 - 01/03/08 09:32 PM (16 years, 29 days ago)

Yes when u make an lc from multispore you will get several substrains growing in ur lc, so inoculating from that lc could still produce variations in each substrate inoculated from it.

I would imagine that you would get less varience in genetics by using very little spore solution in your LC's.

I have used an LC once and had great results from it.But that was just once.

I like LC's because of the fast colinisation. If you take it 1 step further you can grow out 1 substrain in an lc.

Look up cloning and agar.


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