Home | Community | Message Board

Mycohaus
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Left Coast Kratom Buy Kratom Extract   Original Sensible Seeds Bulk Cannabis Seeds   North Spore Bulk Substrate   PhytoExtractum Buy Bali Kratom Powder   Kraken Kratom Red Vein Kratom   Mushroom-Hut Mono Tub Substrate

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlineq pasa
Stranger
Registered: 01/02/08
Posts: 14
Last seen: 15 years, 9 months
I need help
    #7826682 - 01/03/08 08:55 AM (16 years, 29 days ago)

hey people,
I think I need some advice from you, so here is my problem.
I´m a beginner and I´m growing amazonians right now. the first problems came up while colonisation in the jars. after two of the jars where fully colonised the growth of the others more or less stalled and everytime I opend my incubation chamber it smelled more sour. now I read that this smell is probably caused by bacteria contamination, but I didn´t know that at this time. so I just birthed some almost totally colonised cakes and put them in the fruiting chamber with the two cakes that colonised much faster before. two jars left that were hardly colonised after 8 weeks smelled incredible sour so I threw them away. now there is no noticable sour smell in the fruiting chamber. would contaminated cakes lose their sour smell after birthing?? would they even start to fruit? can they influence the healthy ones in the fruiting chamber?
next problem: the ones I birthed first didn´t start to fruit for 3 weeks. one of the cakes started pinning on monday. I´ve had them in my room and they only got the bit of light that came through the window for about 8 hours a day. could this delayed pinning be due to too less light?? I bought a light now anyways.
last question: I´d like to try a different substrate for my next grow. therfore I got some yeast and dextrose. I´m planning to mix it into the flour. is there no danger that my jars could explode while cooking them?? cause my experience in making bread says if I mix yeast with water, sugar and flour it will increase in space...
hope for some advice....
thank you


Extras: Filter Print Post Top
OfflineNebula
Stranger


Registered: 10/22/07
Posts: 172
Last seen: 15 years, 11 months
Re: I need help [Re: q pasa]
    #7826745 - 01/03/08 09:25 AM (16 years, 29 days ago)

Contaminated cakes in the fc can contaminate other cakes in the same fc. I would throw them out as quickly as possible.
Are you sure the cake that first smelled sour, doesn't smell sour anymore? What do you smell if you're holding it right under your nose (don't inhale it too much!).
It's good thing you bought a light, what kind of light is it? A bright fluorescent light is what you need. Less light might have limited your flush, a bright light is needed to initiate pinning.
I find it difficult to comment on your next substrate. Which tek will you follow anyway, a mixture of yeast, flower and dextrose doesn't sound familiar to me, though I could be wrong..


Extras: Filter Print Post Top
InvisibleDontPlay
Yeah Science!
Male


Registered: 12/13/07
Posts: 750
Loc: On Land most of the time....
Re: I need help [Re: Nebula]
    #7826771 - 01/03/08 09:32 AM (16 years, 29 days ago)

Where did you get the idea to add either of those things to your substrate ?


--------------------
My Trade List


I hate to advocate drugs, alcohol, violence, or insanity to anyone, but they've always worked for me. 
    Hunter S. Thompson



I prefer dangerous freedom over peaceful slavery.
~Thomas Jefferson


Edited by DontPlay (01/03/08 09:35 AM)


Extras: Filter Print Post Top
Offlineq pasa
Stranger
Registered: 01/02/08
Posts: 14
Last seen: 15 years, 9 months
Re: I need help [Re: DontPlay]
    #7826800 - 01/03/08 09:41 AM (16 years, 29 days ago)

I read that yeast would speed up colonisation and with dextrose you give them nutricion for the educts of psilocybin synthesis. maybe I´m wrong. just correct me please if so...
thanks


Extras: Filter Print Post Top
OfflineFraggin
Multi-Faceted
 User Gallery


Registered: 01/05/05
Posts: 8,707
Last seen: 8 years, 3 days
Re: I need help [Re: q pasa]
    #7826810 - 01/03/08 09:42 AM (16 years, 29 days ago)

Yeasts excrete alcohol. Not a good thing for mycelium.


Extras: Filter Print Post Top
Offlineq pasa
Stranger
Registered: 01/02/08
Posts: 14
Last seen: 15 years, 9 months
Re: I need help [Re: Fraggin]
    #7827167 - 01/03/08 12:05 PM (16 years, 29 days ago)

I read about nutritional yeast being mixed to the substrate in the forum. man I´m confused now....


Extras: Filter Print Post Top
OfflineNibin
Getting there
Male User Gallery


Registered: 11/29/05
Posts: 4,480
Last seen: 10 years, 8 months
Trusted Cultivator
Re: I need help [Re: q pasa]
    #7827498 - 01/03/08 01:33 PM (16 years, 29 days ago)

Quote:

q pasa said:
I read that yeast would speed up colonisation and with dextrose you give them nutricion for the educts of psilocybin synthesis. maybe I´m wrong. just correct me please if so...
thanks




Dextrose is just plain glucose (it''s the same thing) a basic carbohydrate that the mycelium can synthesize at any time and that in no way is essential for psylobicin production.


--------------------
Newcomers guide-----> For all things shroomy


Extras: Filter Print Post Top
Offlineq pasa
Stranger
Registered: 01/02/08
Posts: 14
Last seen: 15 years, 9 months
Re: I need help [Re: Nibin]
    #7827578 - 01/03/08 01:53 PM (16 years, 29 days ago)

I know that dextrose is d-glucose and that it´s needed for gaining energy. but check this out http://www.shroomery.org/6227/The-Metabolic-Pathway-of-Psilocybin-Production

it says that there´s no psilocybin without glucose. and as dextrose is the kind of glucose which eucaryotic cells prefer, why not give them dextrose?


Extras: Filter Print Post Top
Offlinerobanero
β Lib
Male User Gallery
Registered: 09/05/06
Posts: 874
Loc: Somewhere in Tx
Last seen: 5 years, 8 months
Re: I need help [Re: q pasa]
    #7828474 - 01/03/08 05:38 PM (16 years, 29 days ago)

There are several recipes for agar that cal for yeast. MYP or Malt-Yeast-Peptone medium is a good recipe. I don't know why you couldn't use it in a substrate.

Add to 1 liter of boiling water:

7g malt extract (powder ore syrup)
1 g peptone or sytone
0.5 g yeast extract
for agar add 15 g agar


--------------------

310 open cap Hawaiians grown a 1 pint WBSF cake.

SPAWN RATIO CALCULATOR


My Little Hawaiians

Spawn Bag Tek


Extras: Filter Print Post Top
Offlineq pasa
Stranger
Registered: 01/02/08
Posts: 14
Last seen: 15 years, 9 months
Re: I need help [Re: robanero]
    #7830290 - 01/03/08 11:35 PM (16 years, 29 days ago)

ok I´ve been talking bullshit before. I´m not planning to mix the dextrose into the flour. the dextrose is ment for my liquid media.
but to my other question: is there really high contamination risk if i put sour smelling (probably bacteria) cakes to healthy ones in my fruiting chamber? the fruiting chamber isn´t sterile anyways. that means there´s lots of bacteria in there, right?

thank you!


Extras: Filter Print Post Top
Offlinemushies101
shroomer
Male

Registered: 10/23/07
Posts: 69
Loc: one shitty place
Last seen: 15 years, 11 months
Re: I need help [Re: q pasa]
    #7830350 - 01/04/08 12:00 AM (16 years, 28 days ago)

Quote:

q pasa said:
ok I´ve been talking bullshit before. I´m not planning to mix the dextrose into the flour. the dextrose is ment for my liquid media.
but to my other question: is there really high contamination risk if i put sour smelling (probably bacteria) cakes to healthy ones in my fruiting chamber? the fruiting chamber isn´t sterile anyways. that means there´s lots of bacteria in there, right?

thank you!





If your fruiting chamber isn't sterile then just start over anyway man. You need to read up on this more first. Sterile is the main thing in cultivating this magic fungus.


--------------------
mycologist for life


Extras: Filter Print Post Top
Offlineq pasa
Stranger
Registered: 01/02/08
Posts: 14
Last seen: 15 years, 9 months
Re: I need help [Re: mushies101]
    #7830655 - 01/04/08 03:23 AM (16 years, 28 days ago)

are you serious?????????


Extras: Filter Print Post Top
Offlineq pasa
Stranger
Registered: 01/02/08
Posts: 14
Last seen: 15 years, 9 months
Re: I need help [Re: q pasa]
    #7830659 - 01/04/08 03:25 AM (16 years, 28 days ago)

someone pleeeeeeeaaaaaaaaaaase tell me that´s a joke....


Extras: Filter Print Post Top
Invisiblefastfred
Old Hand
Male User Gallery

Folding@home Statistics
Registered: 05/17/04
Posts: 6,899
Loc: Dark side of the moon
Re: I need help [Re: q pasa]
    #7830680 - 01/04/08 03:50 AM (16 years, 28 days ago)

Quote:

q pasa said:
I know that dextrose is d-glucose and that it´s needed for gaining energy. but check this out http://www.shroomery.org/6227/The-Metabolic-Pathway-of-Psilocybin-Production

it says that there´s no psilocybin without glucose. and as dextrose is the kind of glucose which eucaryotic cells prefer, why not give them dextrose?




You can ignore that link. It's just a bunch of BS.

The pathway prior to tryptophan is irrelavent. All living organisims have a carefully regulated amount of tryptophan in them. And the article doesn't really explain anything after tryptophan --> tryptamine. It doesn't reflect even 1970's era research.

I pointed this out to the author and made a comment in the comments section, but apparently it somehow got deleted.


-FF


Extras: Filter Print Post Top
Offlineveda_sticks
Cultivator
Male User Gallery


Folding@home Statistics
Registered: 07/29/07
Posts: 14,191
Loc: UK Flag
Last seen: 4 years, 25 days
Re: I need help [Re: fastfred]
    #7830713 - 01/04/08 04:32 AM (16 years, 28 days ago)

Quote:

If your fruiting chamber isn't sterile then just start over anyway man. You need to read up on this more first. Sterile is the main thing in cultivating this magic fungus.




I suggest u do some reading. Fruiting chambers do not need to be sterile.

Fully colinised substrate is resistant to contamination. If Fruiting chambers had to be sterile, it would make it very diffucult for most people to grow.

Using a shotgun type FC, with holes in all six sides, how is that sterile?? How is it sterile when u take the lid of and give a good FAE.

However, you should not put a contaminated cake in a fruiting chamber for 2 reasons.

1. there is a higher risk of contaminating your other cakes.
2. risk of spreading spores etc that could contaminate future grows.

Fruiting chambers are not sterile and dont need to be, but that doesnt mean u can fling anyting in there. Keep it clean.


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek


Extras: Filter Print Post Top
Offlineq pasa
Stranger
Registered: 01/02/08
Posts: 14
Last seen: 15 years, 9 months
Re: I need help [Re: veda_sticks]
    #7830907 - 01/04/08 07:20 AM (16 years, 28 days ago)

thanks a lot! I´m new to this forum, but I´ve done a lot reading before.
you helped me a lot! unfortunately people that have never grown before also try to tell you stuff...


Extras: Filter Print Post Top
OfflineNibin
Getting there
Male User Gallery


Registered: 11/29/05
Posts: 4,480
Last seen: 10 years, 8 months
Trusted Cultivator
Re: I need help [Re: fastfred]
    #7832435 - 01/04/08 03:54 PM (16 years, 28 days ago)

Quote:

fastfred said:
Quote:

q pasa said:
I know that dextrose is d-glucose and that it´s needed for gaining energy. but check this out http://www.shroomery.org/6227/The-Metabolic-Pathway-of-Psilocybin-Production

it says that there´s no psilocybin without glucose. and as dextrose is the kind of glucose which eucaryotic cells prefer, why not give them dextrose?




You can ignore that link. It's just a bunch of BS.

The pathway prior to tryptophan is irrelavent. All living organisims have a carefully regulated amount of tryptophan in them. And the article doesn't really explain anything after tryptophan --> tryptamine. It doesn't reflect even 1970's era research.

I pointed this out to the author and made a comment in the comments section, but apparently it somehow got deleted.


-FF




As FastFred said, that link is basically just giving the the basic pathways through which most aminoacids are synthesized, nothing really special or specific to cubes, especially the second part.

The cells in your body all use these routes in fact, your red blood "cells" basically rely on the pentose phosphate route.

Anyway, back to the using glucose (dextrose) in LC, that is one of the two ingedients of Karo, along with fructose (and a tiny amount of salt), and Karo is used as a LC medium.


--------------------
Newcomers guide-----> For all things shroomy


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Left Coast Kratom Buy Kratom Extract   Original Sensible Seeds Bulk Cannabis Seeds   North Spore Bulk Substrate   PhytoExtractum Buy Bali Kratom Powder   Kraken Kratom Red Vein Kratom   Mushroom-Hut Mono Tub Substrate


Similar ThreadsPosterViewsRepliesLast post
* tryptophan > tryptamine > dimethyltryptamine > dimethyltryptaminephosphate Ellezdey 4,815 16 11/07/07 08:41 AM
by RogerRabbit
* tryptamine synthesis from tryptophan darellmatt 1,386 1 11/13/14 12:34 PM
by imjrod
* Tryptamine HCl and making more potent shrooms.
( 1 2 all )
daussaulit 6,827 27 01/04/06 04:07 PM
by FungiFood
* Make shrooms 3 times more potent with Tryptamine HCL
( 1 2 3 4 all )
NeoQ 15,344 62 07/24/06 05:56 AM
by fastfred
* Quinoa//Tryptophan -- Solubility in water... bcfresh 1,934 12 07/10/06 05:00 AM
by bcfresh
* Tryptamine Cubensis krill 2,367 12 01/09/09 02:41 PM
by Psychedelica
* Adding L-Tryptophan to substrate
( 1 2 3 4 5 6 all )
Singularity 9,647 102 11/03/14 04:50 PM
by RogerRabbit
* Re: L-tryptophan and substrate bomb 2,159 7 06/21/00 01:07 AM
by lares

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
1,415 topic views. 16 members, 173 guests and 36 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.024 seconds spending 0.007 seconds on 14 queries.