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Shroom Empire
Myco Matrix




Registered: 12/26/07
Posts: 1,365
Loc: Plugged In
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And 2/3 of the Jars were Smited!
#7822843 - 01/02/08 07:51 AM (16 years, 30 days ago) |
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I birthed 4 fully colonized cakes today, the other 8 contaminated with cobweb and green mold. Separated each contaminated jar from the group each time I spotted a contaminated jar.
I did all of my inoculation procedures inside of a glove box. I misted the outside of the jars and the incubator daily with bleach water.
The jars were ordered from MushBox and the Syringe from Spores 101.
I did everything possible to maintain absolute sterility, including HEPA filtration.
Is it possible that the problem is not on my end?
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veda_sticks
Cultivator




Registered: 07/29/07
Posts: 14,191
Loc: UK
Last seen: 4 years, 25 days
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Re: And 2/3 of the Jars were Smited! [Re: Shroom Empire]
#7822865 - 01/02/08 08:09 AM (16 years, 30 days ago) |
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r those like pf jars?
Did u flame sterilise your needle between inoculations??
-------------------- PF TEK - writeup by EvilMushroom666 Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD Cakes can and will pin! - So you think cakes suck for pins. Your wrong Franks Simple Coir/Verm Tek Franks Proper Pasturisation Tek Franks Spawning To Bulk - Monotub Professor Pinheads RTV Injection Port Tek Foo Mans No Soak WBS Prep Tek
Edited by veda_sticks (01/02/08 08:14 AM)
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04281969
Hobbyist


Registered: 08/09/06
Posts: 1,406
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Re: And 2/3 of the Jars were Smited! [Re: Shroom Empire]
#7822869 - 01/02/08 08:12 AM (16 years, 30 days ago) |
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No, it's you.
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RogerRabbit
Bans for Pleasure



Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 3 days
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Re: And 2/3 of the Jars were Smited! [Re: 04281969]
#7822935 - 01/02/08 09:03 AM (16 years, 30 days ago) |
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What kind of hepa filtration? If you combined a hepa filter with a glovebox, that is very likely the problem. Turbulence is a far greater concern than filtration.
If the syringe was contaminated, all the jars would have failed instead of most of them. Another likely scenario is that the verm barrier on several of the jars shifted during transit.
Lately, I've been making a lot of pf type cakes without the verm barrier. Simply cover the inoculation holes with micropore tape, and then after inoculation, put a second piece of tape over the first. This even lets you fill the jars a bit more with brf. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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Shroom Empire
Myco Matrix




Registered: 12/26/07
Posts: 1,365
Loc: Plugged In
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Re: And 2/3 of the Jars were Smited! [Re: RogerRabbit]
#7823061 - 01/02/08 10:18 AM (16 years, 30 days ago) |
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I do have a hepa filter attached to my glove box.
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veda_sticks
Cultivator




Registered: 07/29/07
Posts: 14,191
Loc: UK
Last seen: 4 years, 25 days
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Re: And 2/3 of the Jars were Smited! [Re: Shroom Empire]
#7823179 - 01/02/08 10:51 AM (16 years, 30 days ago) |
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Well that might be your problem, a glove box is not sterile, but provides an enviroment in which the air is not moving, so contaminants arent floating around and landing on everything.
-------------------- PF TEK - writeup by EvilMushroom666 Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD Cakes can and will pin! - So you think cakes suck for pins. Your wrong Franks Simple Coir/Verm Tek Franks Proper Pasturisation Tek Franks Spawning To Bulk - Monotub Professor Pinheads RTV Injection Port Tek Foo Mans No Soak WBS Prep Tek
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