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goforshrooms
Stranger

Registered: 07/25/07
Posts: 7
Last seen: 15 years, 11 months
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WBS Myco-Bag Tek... need advise!
#7821217 - 01/01/08 07:07 PM (16 years, 1 month ago) |
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Hello there,
I managed to get good quantities of shrooms with PF-Tek, but now I tried to grow by WBS Myco-Bag Tek. I prepare liquid cultures of several Cubensis strains to have live mycelia at hand. Now I'm facing two general problems:
1. Preparing suitable liquid cultures seems to be too difficult... - 240 ml jars - Light Malt Extract - Mixture used is one tablespoon to 250 ml water
No strain came up with mycelia after 2 weeks at 81 °F. So what is the best ratio to use with light malt extract? Do I have to filter the solution before use? Does this solution also work with P. cyanescens?
2. Content of myco-bag starts "brewing"... - Guide used: Click me! - Incubation temperature: 81 °F - Bag fills with gas (from "brewing") and starts to smell foul fast - No mycel growth visible after 2 weeks
My thought, I missed a step in the guide or the used seeds are not the same?! It happened with all 4 bags I tried. The second one (strain: Stargazer) started "brewing" together with the first mycel coming up. There are small dried fruits between my WBS, does this harm the process? The seeds where quite good moisture even after letting the water drain off for over 15 minutes, is this okay? Can someone advise on better preparation of the WBS for growing of Cubensis strains with myco-bags?
Kind regards, Myc
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MYSTIQUE
Say Hi to the elves for me.




Registered: 04/28/07
Posts: 1,764
Loc: Canada
Last seen: 5 years, 4 months
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Re: WBS Myco-Bag Tek... need advise! [Re: goforshrooms]
#7821768 - 01/01/08 09:27 PM (16 years, 1 month ago) |
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Did you rinse, get rid of the floaters. Then soak your WBS for 12 to 24 hours then rinse again? simmer for a hour? load into bags and sterilize in pc? If ya did all that i dont know what ya did wrong
-------------------- Dont know what the fuck I just said? READ THIS http://www.shroomery.org/5122/The-Shroomery-Mushroom-Glossary I ain't a hippy but I'm covered in dirt Sippin lots of mushroom tea in a tye-dye shirt Chasin' the Grateful Dead, no shoes on my feet Beggin' in the parking lot for something to eat, DO NOT USE FIRE IN YOUR GLOVE BOX!!!!!!!
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Blutjager
Inhuman


Registered: 06/11/06
Posts: 9,220
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Re: WBS Myco-Bag Tek... need advise! [Re: MYSTIQUE]
#7821784 - 01/01/08 09:32 PM (16 years, 1 month ago) |
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Pics ??
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goforshrooms
Stranger

Registered: 07/25/07
Posts: 7
Last seen: 15 years, 11 months
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Re: WBS Myco-Bag Tek... need advise! [Re: Blutjager]
#7824534 - 01/02/08 05:42 PM (16 years, 30 days ago) |
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Thanks for your replies.
I did not get rid of sunflower seeds and other floaters and I just let the WBS soak before pc. So I will follow you advise and simmer too.
@Blutjager: Don't have pics of the last bags, but I will take some if further bags will show the same 'brewing'. For now I can only provide pics of the bag I inoculated today with Cubensis 'Virgin Islands' strain today...



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FooMan




Registered: 02/02/05
Posts: 8,957
Loc: Earth
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Re: WBS Myco-Bag Tek... need advise! [Re: goforshrooms]
#7824834 - 01/02/08 06:47 PM (16 years, 30 days ago) |
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I don't bother with long soaks. You can see the link in my sig for how I do my WBS. As far as your LC goes, I would try Karo or turbinado sugar (Sugar in The Raw) from the grocery store. Both work well at about 3-5% of your water.
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Quick WBS Prep
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goforshrooms
Stranger

Registered: 07/25/07
Posts: 7
Last seen: 15 years, 11 months
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Re: WBS Myco-Bag Tek... need advise! [Re: FooMan]
#7828413 - 01/03/08 05:16 PM (16 years, 29 days ago) |
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Thanks for your suggestion Foo, but it seems that Karo is not available in my region oO What is turbinado sugar, can't imagine. Could you please describe it more detailed? Lack of language skills ^^ I think I will use honey (easy to get) as described in BlutJagers tek.
Concerning the WBS preparation. Is there a noticeable difference between normal WBS, finch or other seeds? There are so many different kinds at the market I get them. It's really hard to decide, are there specific criteria for a good seed?
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goforshrooms
Stranger

Registered: 07/25/07
Posts: 7
Last seen: 15 years, 11 months
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Re: WBS Myco-Bag Tek... need advise! [Re: goforshrooms]
#7836647 - 01/05/08 05:49 PM (16 years, 27 days ago) |
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Also one of the first questions not answered yet. Is it possible to make LC with P. cyanescens? Someone experienced problems?
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FooMan




Registered: 02/02/05
Posts: 8,957
Loc: Earth
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Re: WBS Myco-Bag Tek... need advise! [Re: goforshrooms]
#7838443 - 01/06/08 08:20 AM (16 years, 26 days ago) |
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Quote:
goforshrooms said: Thanks for your suggestion Foo, but it seems that Karo is not available in my region oO What is turbinado sugar, can't imagine. Could you please describe it more detailed? Lack of language skills ^^ I think I will use honey (easy to get) as described in BlutJagers tek.
I found the turbinado at the local grocery store. Search posts for the username "Agar" for "sugar in the raw" or "turbinado" for more information.
Quote:
Concerning the WBS preparation. Is there a noticeable difference between normal WBS, finch or other seeds? There are so many different kinds at the market I get them. It's really hard to decide, are there specific criteria for a good seed?
I haven't had any problem using that method regardless of the seed used and I have used about 4 different brands of WBS and 2 different types of finch seed. Some wild bird seeds contain more sunflower seeds than others, but I just pour off all of the seeds that float and use the rest.
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Quick WBS Prep
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goforshrooms
Stranger

Registered: 07/25/07
Posts: 7
Last seen: 15 years, 11 months
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Re: WBS Myco-Bag Tek... need advise! [Re: FooMan]
#7842405 - 01/07/08 06:03 AM (16 years, 25 days ago) |
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@Foo: Thanks again. Just found it at the grocery around the corner of my office. But quite expensive compared to honey. So you would use about 5 g of turbinado sugar on one litre of water?
BTW my last bag also started to stink after some days There was not that much gas inside as with the last bags, but it just stank awful. I will now prepare some wbs with your tek and get rid of the floaters.
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MYSTIQUE
Say Hi to the elves for me.




Registered: 04/28/07
Posts: 1,764
Loc: Canada
Last seen: 5 years, 4 months
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Re: WBS Myco-Bag Tek... need advise! [Re: goforshrooms]
#7842414 - 01/07/08 06:10 AM (16 years, 25 days ago) |
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Let it do a atleast a 12 Hour soak So you can kill those endo spores and you wont get that stink in a day or two. A Soak is the second most important part of the sterilization of grains I find right after PC. I have done wbs with out simmer just wbs and water in a jar then I did simmered and not soaked and I did soak simmer and PC. The only jars that didn't goto Bactria where the soaked jars.
-------------------- Dont know what the fuck I just said? READ THIS http://www.shroomery.org/5122/The-Shroomery-Mushroom-Glossary I ain't a hippy but I'm covered in dirt Sippin lots of mushroom tea in a tye-dye shirt Chasin' the Grateful Dead, no shoes on my feet Beggin' in the parking lot for something to eat, DO NOT USE FIRE IN YOUR GLOVE BOX!!!!!!!
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FooMan




Registered: 02/02/05
Posts: 8,957
Loc: Earth
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Re: WBS Myco-Bag Tek... need advise! [Re: goforshrooms]
#7842429 - 01/07/08 06:16 AM (16 years, 25 days ago) |
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I think the cost between the honey and turbinado is pretty close considering how many packets of turbinado come in each box. I followed Agar's directions and used 1 packet (5 grams) to 220ml's of water. If you're getting contams, you need to increase your sterilization times. Floaters shouldn't make much difference as far as contaminants go.
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Quick WBS Prep
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MYSTIQUE
Say Hi to the elves for me.




Registered: 04/28/07
Posts: 1,764
Loc: Canada
Last seen: 5 years, 4 months
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Re: WBS Myco-Bag Tek... need advise! [Re: FooMan]
#7842447 - 01/07/08 06:29 AM (16 years, 25 days ago) |
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You can get sugar in the raw packets all over around here in the coffe shops and stuff and isnt that turbinado? Do you have make you own wine or make your own beer places around there? They use turbinado to feed the yeast.
-------------------- Dont know what the fuck I just said? READ THIS http://www.shroomery.org/5122/The-Shroomery-Mushroom-Glossary I ain't a hippy but I'm covered in dirt Sippin lots of mushroom tea in a tye-dye shirt Chasin' the Grateful Dead, no shoes on my feet Beggin' in the parking lot for something to eat, DO NOT USE FIRE IN YOUR GLOVE BOX!!!!!!!
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veda_sticks
Cultivator




Registered: 07/29/07
Posts: 14,191
Loc: UK
Last seen: 4 years, 25 days
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Re: WBS Myco-Bag Tek... need advise! [Re: MYSTIQUE]
#7842517 - 01/07/08 07:31 AM (16 years, 25 days ago) |
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If your bags stink, its infected with bacteria.
Soaking your seed helps germinate endospores making it easier to kill them when you pressure cook. Some endospores are quite resistant to heat and can survice pressure cooking. Which is why soaking is recomended, once they have germinated the bacteria can be killed.
Increasing your PC time can also help, how long are you PC and is your PC 15 psi?
-------------------- PF TEK - writeup by EvilMushroom666 Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD Cakes can and will pin! - So you think cakes suck for pins. Your wrong Franks Simple Coir/Verm Tek Franks Proper Pasturisation Tek Franks Spawning To Bulk - Monotub Professor Pinheads RTV Injection Port Tek Foo Mans No Soak WBS Prep Tek
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goforshrooms
Stranger

Registered: 07/25/07
Posts: 7
Last seen: 15 years, 11 months
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Re: WBS Myco-Bag Tek... need advise! [Re: veda_sticks]
#7858253 - 01/10/08 09:03 AM (16 years, 22 days ago) |
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Hey there,
Thanks again for all the info.
I prepared some LC with turbinado now and just wait to see the outcome. Inoculated with P. cubensis 'Mexican'/'Virgin Island' strain and P. cyanescens spores. I will report how they do over the next days.
Concerning the WBS I now prepared a bag with finch seeds. Steamed and let them soak for about 24 hours. My PC is quite old and I just heated to much by accident, so that the bag just smelted away *argh* Was my last bag, so now I will have to wait for my next shipment to be able to give another try. Wanted to let them PC for about 1 1/2 hours... is that sufficient?
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Nibin
Getting there



Registered: 11/29/05
Posts: 4,480
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Re: WBS Myco-Bag Tek... need advise! [Re: goforshrooms]
#7858268 - 01/10/08 09:08 AM (16 years, 22 days ago) |
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Quote:
goforshrooms said: Also one of the first questions not answered yet. Is it possible to make LC with P. cyanescens? Someone experienced problems?
Remember to differentiante between Psylocibe Cyanescens and Paneolus Cyanescens.
P. Cyanescens could be either.
-------------------- Newcomers guide-----> For all things shroomy
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goforshrooms
Stranger

Registered: 07/25/07
Posts: 7
Last seen: 15 years, 11 months
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Re: WBS Myco-Bag Tek... need advise! [Re: Nibin]
#7884763 - 01/15/08 05:42 PM (16 years, 17 days ago) |
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Hey there,
the LCs prepared with turbinado sugar are doing really good! Thank you very much again for your help! Here are some pictures taken after four days...
Psilocybe cubensis "Mexican":

Psilocybe cubensis "Virgin Islands":

Psilocybe cyanescens:

Psilocybe cyanescens (thanks to Nibin for the reminder) seems to grow quite slow. Does this species need special solution as it's a woodloving species? Or is it normal that it needs longer than P. cubensis strains?
Unfortunately my supplier is out of bags, so I need to wait some days more to try it again. Hopefully I will do everything correct the next time...
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