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OfflineCulland
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Registered: 09/12/07
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How long till you give up on a jar?
    #7820338 - 01/01/08 03:02 PM (16 years, 1 month ago)

I bought a syringe of lion's mane and ended up with a syringe of pink oyster sent to me while I waited on the lion's mane. I shot up some grain jars directly with it. Nothing has happened to them at all since Dec 9th. I opened one up and had a smell, no foul smell.

When I received the lion's mane and shot up some jars on Dec 21st and it also has done nothing. With the lion's mane I did two agar plates at the same time, and also nothing on those.

My own liquid cultures in the past have colonized faster then this, so not sure if I did something noobish or just got some bad syringes. How long do folks wait on a jar before giving up?

Thanks,

Cul


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Offlinecasgoodie
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Registered: 10/31/06
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Re: How long till you give up on a jar? [Re: Culland]
    #7820971 - 01/01/08 06:06 PM (16 years, 1 month ago)

are they incubated at appropriate conditions?
i've waited a few weeks
with no signs of germination
and then raised the temps a little and they started.


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OfflineRogerRabbitM
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Re: How long till you give up on a jar? [Re: casgoodie]
    #7820993 - 01/01/08 06:11 PM (16 years, 1 month ago)

With liquid cultures, they should be off and running within a few days. I'd contact the supplier and report what you reported here. Noob mistakes would often result in contamination, but hardly ever in no growth at all. My oyster and lion's mane cultures will fully colonize a quart jar of rye in ten days.
RR


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OfflineCulland
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Re: How long till you give up on a jar? [Re: RogerRabbit]
    #7821283 - 01/01/08 07:26 PM (16 years, 1 month ago)

Yea its in a TiT incubator and my birch polypore and shaggy mane both seem to like it in there, as did my pearl oyster spawn. Germinating spores does take awhile, but these were liquid cultures and my own liquid cultures are a lot like what you describe RR, which is why I question these two syringes. There is no sign of contams in either lions mane or pink oyster jars, no sign of anything in fact.

Oh well, let the vendor know and move on.

Cul


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