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punkhardcore92
The navigator


Registered: 06/03/07
Posts: 494
Loc: Oregon
Last seen: 12 years, 29 days
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Big,Fat, PHAIL(now trying HoneyLC)
#7815952 - 12/31/07 04:10 AM (16 years, 1 month ago) |
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Well the Mazatapec was a bust, I lost every single jar to too much moisture soon followed by contams. The first attempt, I did it EXACTLY like my successful cambo's and John Allen's(this means following the popcorn tek verbatim), For some reason after PCing there was stagnant water in the bottom of the jars DESPITE drying thoroughly for half an hour. ALL kernels were dry to the touch before PC'd
I figured it was because of of the holes in the lids under the micro pore tape and under the aluminum foil. Humidity was getting in there so i PC'd new jars without any holes, Still the same problem followed by contams.
Alright i say, time to kick it up a notch, I took the next batch of corn that soaked for 24 hours and super dried it all with paper towels AFTER being air dried then i put it in a refrigerator for an hour, just long enough to condensate and whipped all the kernels again. STILL too much water, followed by contams
AT this point I'm pretty belligerent and not thinking clearly, I decided i was going to cut the soaking step right out of the popcorn tek and just skip straight to boiling, After the corn boiled i dried the kernels out with paper towels, put them in a jar with the lid closed and threw it in the fridge for an hour till condensation appeared, removed the corn, dried the corn, introduced the corn into new dry jars, PC'd for an hour @ 15 psi. THIS time i didn't have stagnant water but the kernels still looked wet and pressed up against the glass in more than just a "few" spots. Some promising growth started form followed by pink bacteria and nothing but a big heaping serving of failure.
I decided that i hate corn and was moving to mung beans, i didn't want moisture problems so i skipped right to boiling the mung beans, drying them, then pressure cooking. Perfect moisture content, had great growth forming then the jars turned and fermented. BLASPHEMY!!!! SO hear i am, down a syringe of mazatapec, with only 7cc or 6cc's of john Allen left. Broke, frustrated, what to do??? Regroup and visit the shroomery!
So after about a week of intensive 5am post searching, I've come to the conclusion the best thing for me to do is begin my experiments with LC, I know a lot of people don't like LC other people swear by it. Its one of those things I'm going to have to play with to try to see if i can recover some of my production capacity I've lost and perhaps exponentially increase my myc supply to SOON return to the world of grains once this ominous bad luck cloud has passed over me to some other unsuspecting beginning mycologist
So first things first, I've already got the 250ml of water with a table spoon of honey PC'd and cooling, waiting for morning in the PC till i finish constructing the new glove box!, I have a feeling my contams were coming from my crappier makeshift glovebox I've used for the past year. Here are my new G/B materials! sitting on top of the old glove box.

My old glove box had a textured bottom and a solid white top so i had to put the glove's on the bottom of the box to see through the sides, as you can imagine that doesn't work great because now the top of the box is one of the sides and everything becomes harder to work with, This new glove box will be simple construction. Gloves in side, view through the top, Syringe, lighter, and disinfectant waiting to go inside!.
Also with my new found LC adventure I'm going to change my sterile technique up, This glove box is bigger than my last now allowing me to put the entire pressure cooker inside before i even open it. So my entire procedure will be:
Boil water with the pint glass jar and lid in it, leave 250ml of boiling water in the jar and add a tablespoon of honey. Put the Lid on the jar followed by aluminum foil, Pressure cook for 30mins at 15psi. While this is cooling I'll get my glove box in order, Make sure all the needed supplies are inside it. Once the PC is cooled i plan to move the ENTIRE PC into my glove box, Sealing the glove box, Disinfecting, letting sit for 10 mins, Then I'll open the PC.... I think alot of my contams come from me taking the jars OUT of the pc then putting them in the glove box. This may solve that. Then of course i can immediately inoculate the jars. Throw them in the incubator at 82-84 degree's and see if i can't turn my mycelium capacity back around. Yes i know sugar based LC's fail but its still hope.
-------------------- Guns, the only thing more dangerous is not having them. Support the 2nd amendment or give up all your civil liberties. Everything said by me is fiction.
Edited by punkhardcore92 (01/01/08 08:22 PM)
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veda_sticks
Cultivator




Registered: 07/29/07
Posts: 14,191
Loc: UK
Last seen: 4 years, 25 days
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I cant comment on why you have had so much trouble using grains as i used popcorn once, my problem was green mould, but that was due to not having any tyvek or micropore tape.
You shouldnt have any problems transfering your jars from the pressure cooker into the glove box, the foil will stop anything landing on the lids or getting in the inoc holes, to be extra certain you can wipe the jar lids with alcohol.
Be very carefull flaming your needle inside a glovebox containing disinfecting most are flammable. I have read a few posts about gloveboxes exploding.
-------------------- PF TEK - writeup by EvilMushroom666 Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD Cakes can and will pin! - So you think cakes suck for pins. Your wrong Franks Simple Coir/Verm Tek Franks Proper Pasturisation Tek Franks Spawning To Bulk - Monotub Professor Pinheads RTV Injection Port Tek Foo Mans No Soak WBS Prep Tek
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Sillicybin
Registered: 02/14/05
Posts: 2,134
Loc:
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I would not worry about putting the PC in your glovebox. What you want to do is transfer the jars into your glovebox with all your tools, let them cool to room temperature, and then begin work. If there are any temperature differences inside the box, it'll cause convection drafts.
Opening your PC inside the glovebox will make everything wet and moist inside. You want everything as dry as possible.
If you're using Tyvek, you don't have to worry about contaminants landing on the outside of the jars much.
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doc34
Fungitarian



Registered: 02/14/04
Posts: 2,667
Loc: Myceliaville !!!
Last seen: 7 months, 29 days
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Quote:
Boil water with the pint glass jar and lid in it, leave 250ml of boiling water in the jar and add a tablespoon of honey. Put the Lid on the jar followed by aluminum foil, Pressure cook for 30mins at 15psi.
You may want to decrease that to 20 minutes, honey has a bad habit of turning a dark color and or caramalizing(From my past exp with it). 30 minutes works for some, some it doesn't-just thought I would say that.
My two pennies
Doc
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punkhardcore92
The navigator


Registered: 06/03/07
Posts: 494
Loc: Oregon
Last seen: 12 years, 29 days
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Re: Big,Fat, PHAIL [Re: doc34]
#7818825 - 01/01/08 01:04 AM (16 years, 1 month ago) |
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Oh yeah, that honey, definitely carmalized, I probably will try again tonight. Now that i have multiple jars :O, Ima do a jar of Karo, and 2 more jars of honey for 15mins ONCE the wieght is spinning. Its funny i've NEVER had grain problems before, Must be something growing in my room some where in one of the walls :S.
What sucks about tonights failed LC is i didn't notice till AFTER i inoculated it that it was carmalized, so it probably wont grow or have some crazy strange mutations. So tonight, Round 2 with the LC's. Here are pics of MORE of my FAILURES. Im not surprized, I think i had too much sucess last year and karma is just evening it out.
PICTURES AS PROMISED



Think anything will grow?
-------------------- Guns, the only thing more dangerous is not having them. Support the 2nd amendment or give up all your civil liberties. Everything said by me is fiction.
Edited by punkhardcore92 (01/01/08 01:25 AM)
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punkhardcore92
The navigator


Registered: 06/03/07
Posts: 494
Loc: Oregon
Last seen: 12 years, 29 days
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*update* 7 25 in the morning, Cooking at 15psi, Removing jars at 7 40. Decided to go with 4 more jars of this honey water LC, apparently the floating stuff after the PC has a good chance of being "Sediments" and more of my late night forum searching (and battling with how to word things for the search engine) I've come to the conclusion that these sediments are remainders of natural wax's found in this 100% natural, harvested on the other side of town, honey. After PC I'm going to bed letting cool over night (morning) and inoculating @ awakening.
-------------------- Guns, the only thing more dangerous is not having them. Support the 2nd amendment or give up all your civil liberties. Everything said by me is fiction.
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sproket13
Stranger



Registered: 10/26/05
Posts: 571
Loc: FL
Last seen: 10 years, 7 months
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i found karo to be much better...
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tahoe
Noob Slayer



Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
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Re: Big,Fat, PHAIL [Re: sproket13]
#7819733 - 01/01/08 11:31 AM (16 years, 1 month ago) |
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I have found that tan malt is better then all others for lc's.
I would never expect a syringe to be clean enough for lc.
I used small mouth quarts with my lc with 500ml and about a 1/2 teaspoon of malt.
You guys make your lc's too rich
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
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Nibin
Getting there



Registered: 11/29/05
Posts: 4,480
Last seen: 10 years, 8 months
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Re: Big,Fat, PHAIL [Re: tahoe]
#7820101 - 01/01/08 01:39 PM (16 years, 1 month ago) |
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Quote:
tahoe said: I have found that tan malt is better then all others for lc's.
I would never expect a syringe to be clean enough for lc.
I used small mouth quarts with my lc with 500ml and about a 1/2 teaspoon of malt.
You guys make your lc's too rich
Tan malt Tahoe? Can you elaborate further please? I'm interested...
-------------------- Newcomers guide-----> For all things shroomy
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tahoe
Noob Slayer



Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
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Re: Big,Fat, PHAIL [Re: Nibin]
#7820137 - 01/01/08 01:50 PM (16 years, 1 month ago) |
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really?? you do not know what tan malt is?
it is barley malt from a brewery supply store. it is the lightest (in color) malt that you can get. Tan/extra light.
Think of lc's as agar without the agar. just about anything will work for an lc but what will work great.
MEA calls for 20 grams of malt per 1000ml's of water. So you could make this without the agar and have a very rich lc. I make my lc's with 1000ml of water and >5 grams of malt about a teaspoon. Less is better with lc's.
The lc's come out almost clear when pc'ed at 15 psi for 20 mins
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
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Nibin
Getting there



Registered: 11/29/05
Posts: 4,480
Last seen: 10 years, 8 months
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Re: Big,Fat, PHAIL [Re: tahoe]
#7820151 - 01/01/08 01:54 PM (16 years, 1 month ago) |
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Quote:
tahoe said: really?? you do not know what tan malt is?
it is barley malt from a brewery supply store. it is the lightest (in color) malt that you can get. Tan/extra light.
The lc's come out almost clear when pc'ed at 15 psi for 20 mins
I have lived most of my life in Spain, and all the brewing terminology I know is in Spanish. And I had never heard that name before, in the past I had heard people refer to it as "use the lightest malt you can get".
-------------------- Newcomers guide-----> For all things shroomy
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Mallanaga
Stranger
Registered: 01/01/08
Posts: 1
Last seen: 16 years, 1 month
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Re: Big,Fat, PHAIL [Re: Nibin]
#7820530 - 01/01/08 03:51 PM (16 years, 1 month ago) |
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hi. super noob here, and just trolling around. this is my first post, and it's going to be a question. lot's more to follow, trust me.
what are you guys doing here? with the malt / honey stuff...
you mix spores with it to make syringes? thanks ahead of time.
nice forum you have here
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punkhardcore92
The navigator


Registered: 06/03/07
Posts: 494
Loc: Oregon
Last seen: 12 years, 29 days
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Re: Big,Fat, PHAIL [Re: Mallanaga]
#7821520 - 01/01/08 08:21 PM (16 years, 1 month ago) |
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welcome to the forum, What i am trying to accomplish here, Is take a spore syringe, and increase my capacity for mycellium production through growing quanities in a honey/water suspension. THere happends to be a brewing supply store 5 mins from my house. Im going to have to investigate this "malt". If your intersted in Honey LC try this
http://www.shroomery.org/forums/showflat.php/Cat/0/Number/5238137/page/0/fpart/1/vc/1 http://www.shroomery.org/forums/showflat.php/Number/7423273#7423273
aparently the problem with these LC's is you wont know its contaminated until after you tried to use the myc on whatever substrate you are using. Aparently its smart to test your LC on small quanities of substrate before going apeshit and loosing alot of grain or whatnot that you choose to use.
Its all a huge gamble to me, Now im out of spores in my syringes and if none of these jars work then i'll take some downtime, regroup, and try again in the spring after im done growing CHRONIC PHAT PORNSTAR NUGGS UNDER 600WATTS OF HPS
<object width="425" height="355"><param name="movie" value="&rel=1"></param><param name="wmode" value="transparent"></param><embed src="&rel=1" type="application/x-shockwave-flash" wmode="transparent" width="425" height="355"></embed></object>
after all that has been stealing my focus all winster :S
-------------------- Guns, the only thing more dangerous is not having them. Support the 2nd amendment or give up all your civil liberties. Everything said by me is fiction.
Edited by punkhardcore92 (01/01/08 08:25 PM)
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