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blood4blood
Calmer Than You Are


Registered: 04/25/07
Posts: 6,029
Loc: The Valley
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question on agar's grain lc tek
#7815919 - 12/31/07 03:29 AM (16 years, 1 month ago) |
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im about to do this tek
http://www.shroomery.org/forums/showflat.php/Cat/0/Number/6817701/an/0/page/0
and realized that i have way to much grain in my jars to fit 180 ml of water into it. so i was wondering what the best method for getting some grains out of the jar without comprimising the sterility of the jar?
could i break them up and put half of them into another jar somehow?
how would one go about sterilizing a completly empty jar?
help me out guys
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punkhardcore92
The navigator


Registered: 06/03/07
Posts: 494
Loc: Oregon
Last seen: 12 years, 30 days
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Re: question on agar's grain lc tek [Re: blood4blood]
#7815954 - 12/31/07 04:15 AM (16 years, 1 month ago) |
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Can you preform the entire proceedure in a glove box? The grains you remove from your jar and introduce into the other jar will probably be need to be retreated, but the grains remaining in your jar should be fine to do whatever with. No matter what you do, anything that involves opening the jar has got to compromise things on SOME level. Just if you were to do it in a controllable environment like a glove box you may be able to figure out something.
Funny thing is I'm in the process of switching over to Agars LC's just recently. I've been having horrible grain failures which have never occurred before only just recently. LC's may be the answer
-------------------- Guns, the only thing more dangerous is not having them. Support the 2nd amendment or give up all your civil liberties. Everything said by me is fiction.
Edited by punkhardcore92 (12/31/07 04:19 AM)
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blood4blood
Calmer Than You Are


Registered: 04/25/07
Posts: 6,029
Loc: The Valley
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yea my plan is to sterilize 4 qrt. jars in my pc. 2 of them empty and two of them half full of water. im sterilizing 2 emptys because my colonized grain jar is a regular mouth with a filter disk and the 2 emptys will be widemouths with lc lids whitch will be alot easier to work with.
i plan breaking the colonized jar up and splitting that into the 2 empty sterilized jars and basically ill be making two 500 ml (roughly) lc's. all the grain transfers will be done in a glove box. but i plan on doing the rest in open air because messing with a 60ml syringe in a glove box will suck.
if anybody sees any flaws in this method please let me know.
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blood4blood
Calmer Than You Are


Registered: 04/25/07
Posts: 6,029
Loc: The Valley
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Re: question on agar's grain lc tek [Re: blood4blood]
#7816792 - 12/31/07 11:56 AM (16 years, 1 month ago) |
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so you guys think this should work without any contamination issues?
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Nibin
Getting there



Registered: 11/29/05
Posts: 4,480
Last seen: 10 years, 8 months
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Re: question on agar's grain lc tek [Re: blood4blood]
#7817001 - 12/31/07 12:58 PM (16 years, 1 month ago) |
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Why don't you just do it in various batches. Put in 50ccs at a time and do it as many times as you need to.
-------------------- Newcomers guide-----> For all things shroomy
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blood4blood
Calmer Than You Are


Registered: 04/25/07
Posts: 6,029
Loc: The Valley
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Re: question on agar's grain lc tek [Re: Nibin]
#7817019 - 12/31/07 01:06 PM (16 years, 1 month ago) |
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do you think it will be to much of a hassel to do this with my grain jar setup? its a regular mouth qrt jar with the metal lid on (4 small holes punched in the lid) and a filter disk over the top of that.
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