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OfflineMagicius
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Registered: 12/30/07
Posts: 62
Last seen: 15 years, 8 months
increasing the rate of colonization: ways?
    #7815483 - 12/30/07 11:00 PM (16 years, 1 month ago)

I don't know how advanced this is: but on Shroomery I've seen some dude mixing I think coffee into his casing I think, and his Rhizomorphs looked all crazy like they wanted to climb out the tray, though I've seen that witout coffee, just moisture on the sides...

Do you guys have any ideas on ways to stimulate and provoke faster colonization; that is, say you have a genetically 'slow' athlete. How can you train the bugger to run faster, and, what would you give such an athlete to permanently increase his health, if you can only feed him once twice or thrice.

Here's an interesting link about beta-adenosine causing faster fruitings on oyster mushrooms and stropharia ruggoso annulata,

http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6TH7-4BD5C0R-3&_user=10&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=aa1432d3b3231b2c6df2a850c28ad94f

and I quote :
" Supplementation of wheat straw with 30% Lolium perenne grass chaff enhances the fructification and yield of the edible mushrooms Pleurotus pulmonarius and Stropharia rugosoannulata. We have identified β-adenosine as one of the bioactive compounds in grass chaff that stimulates fruit body formation and increases yield"

have any of you guys used grass chaff with noticible results. Also I read on wikipedia that caffeine molecule is similar to adenosine molecule. I don't exactly know how similar, they didn't say.

But I *AM* looking for ways to ***SIGNIFICANTLY*** speed up colonization time.

Of course a practical method I read out of the book review of mycelium running (P. Stamets) is that rather than mixing up your spawn into the substrate, it colonizer faster when spawned in horizontal layers, inbetween the layers of spawn theres uncolonized substrate; the layes grow towards each other and fuse.

But that is nice to know, but still dis not really answer my question, regarding how to make the mycelium itself, the hyphae, themselves, grow faster, and not necessarily 'colonize' faster. I think that because of that quote from Mr. Stamets, there is definitely a distinction to be drawn between the rates of hyphal growth and colonization; they are not the same thing, in this sense, if you consider that spawn layering approach that increasing colonization speeds, but does not make the hyphae themselves grow faster, per se.

Has anyone perhaps added some powdered vitamin / dissolved vitamin tablets, iron tablets, etc. maybe, into their substrate moistures?

Another nice thing about that beta adenosine from grass chaff is it apparently massively increases stropharia ruggoso annulata harvest, but also triggered a pinset that was 18 days earlier than usual!

I will definitely hvae to test that. But do you guys have any 'secrets' you're willing to let ol' Magicius in on? Yz? No?

)/_
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\L..' ._O__)_
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Invisiblezyxwvutsr
rstuvwxyz

Registered: 12/30/07
Posts: 141
Re: increasing the rate of colonization: ways? [Re: Magicius]
    #7815511 - 12/30/07 11:12 PM (16 years, 1 month ago)

inoculate yourself an agar plate with your favorite strain and isolate the most rhizomorphic mycelium. make a liquid culture with a chunk of mycelium and a marble in a baby food jar (shaking purposes)
use only a small portion to inoculate your jar/mycobag* (<- * if so make your own pref. grain/rye). spawn to hpoo. case. fruit. harvest. CLONE.

"it colonizer faster when spawned in horizontal layers, inbetween the layers of spawn theres uncolonized substrate; the layes grow towards each other and fuse. "

TRUE when using an isolated single strain. start off with a multispore solution forget it.


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OfflineRogerRabbitM
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Registered: 03/26/03
Posts: 42,214
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Re: increasing the rate of colonization: ways? [Re: zyxwvutsr]
    #7815574 - 12/30/07 11:46 PM (16 years, 1 month ago)

Quote:

I read out of the book review of mycelium running (P. Stamets) is that rather than mixing up your spawn into the substrate, it colonizer faster when spawned in horizontal layers, in between the layers of spawn theres uncolonized substrate; the layes grow towards each other and fuse.





I've been saying layering works better and faster than mixing for years around here. It's good to see Paul notice that as well.

Generally, with the exception of coffee, nutrients you add to a substrate will slow down colonization, rather than speed it up. The least nutritious substrates, such as straw, colonize fastest. If the mycelium is hungry, it will grow faster and more rhizomorphic in search of food. If you provide a very rich substrate, the mycelium will colonize it, only slower.

If you want genetics for very fast growth, you'll have to isolate very fast growing sectors on agar and transfer these to your substrates. However, the fastest growing cell lines might not be the cell lines that provide the other qualities you're seeking.
RR


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"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
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OfflineMagicius
Stranger

Registered: 12/30/07
Posts: 62
Last seen: 15 years, 8 months
Re: increasing the rate of colonization: ways? [Re: RogerRabbit]
    #7815686 - 12/31/07 12:48 AM (16 years, 1 month ago)

so basically if you feed your mycelium too much; your mycelium will be obese and lethargic like, oh say, a 'large' person, who generally have weak knees because of their weight and thus cannot risk running too fast; and if you give them just enough food (your mycelium); they'll be fleet of foot? I mean I know mycelium cannot build up 'fat' deposits.

Has anyone ever taken microscopic photo's of well-fed hyphae vs. just barely fed mycelium, i.e eat to live vs live to eat mycelium? sorry if this sounds weird/ just plain dumb

ButI guess they'd spend more time processing nutes maybe, rather than seeking more food elsewhere?

Have u seen Blue Helix' poo/WBS (50/50 supplemented with mustard seed, pollin, and some oils. He inoculates a bag with 140 ml LC and in 25 days he has harvest, from the day he makes up the lc.

That *is* a large amount of LC. Also I noticed he's deleted that post, or rather, that access is denied... =[


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