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InvisibleRipple
Ripple
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Registered: 05/16/02
Posts: 21,014
Loc: the timbers of Fennario
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Making My first Prints
    #781466 - 07/28/02 07:27 AM (14 years, 8 months ago)

The FAQ's here have a very simple method "How can I make a sporeprint?" which seems real easy. However some of the links to other methods are a bit more complex. Can I please get some input on the method in the FAQ's, does it work or should I use one of the more complex methods to keep out contams?

Thanks


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OfflineFanaTEK
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Re: Making My first Prints [Re: Ripple]
    #781536 - 07/28/02 08:37 AM (14 years, 8 months ago)

All of those methods work if you do them properly - keeping out contams is really a function of how careful, quick, and attentive to sterilie technique you are.

FanaTEK


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InvisibleRipple
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Re: Making My first Prints [Re: FanaTEK]
    #781547 - 07/28/02 08:44 AM (14 years, 8 months ago)

But it seems like keeping out contams is not really a main concern in the FAQ tek it just has you put the cap on a piece of paper under a glass for 12 hrs. the other teks are much more attentive to keeping out contams.


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OfflineFanaTEK
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Re: Making My first Prints [Re: Ripple]
    #781583 - 07/28/02 09:15 AM (14 years, 8 months ago)

Usually what I tell people in this instance is to pick a tek that they think they can do without having to modify or fudge any of it (ie. uses materials available to you, you understand all the directions, etc.).

As for sterile technique, that's something you really have to pay attention to in spite of whatever the teks might include. Many of these techniques ASSUME that you are doing things like spraying with lysol, washing hands, sterilizing equipment and work area with alcohol or some sort of disinfectant, closing off sources of draft and air current (like doors, windows, fans, and HVAC vents) or working inside a glove box or in front of a flow hood.

Your sterile technique is really dependant on your need for it and your ability to adhere to it. Some older houses are 'infected' with different kinds of molds or bacteria, so a greater degree of sterility is necessary for success in some cases and not others.

You truly can't be clean enough though, no matter what the tek says. For printing and syringe making, it's probably worth the trouble to construct a glove box.

FanaTEK


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Read the FAQ!
Read !


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InvisibleRipple
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Re: Making My first Prints [Re: FanaTEK]
    #781592 - 07/28/02 09:25 AM (14 years, 8 months ago)

Wow, Thanks, do you feel that it is more important to maintain these clean teks at the print making stage than during innoculation. Or are both just as important.
I may have just answered my own question!


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OfflineFanaTEK
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Re: Making My first Prints [Re: Ripple]
    #781676 - 07/28/02 10:30 AM (14 years, 8 months ago)

>>I may have just answered my own question!<<

Yep, you sure did. I will say, though, that sterility is MOST vital during inoculation, spore printing, and syringe making. Colonized substrate is faily contam-resistant, though the cleaner and faster you work the better for all stages of mushroom development.

FanaTEK


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Read !


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Offlinebloo_sunshine
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Registered: 04/24/02
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Re: Making My first Prints [Re: FanaTEK]
    #781784 - 07/28/02 11:39 AM (14 years, 8 months ago)

^agrees^

Work in a small area and give it a few sprays of lysol here and there. Wipe it down with some alcohol.

Good Luck


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Invisiblematts
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[Re: bloo_sunshine]
    #782056 - 07/28/02 01:46 PM (14 years, 8 months ago)



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