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Offlinesmokeydablunt
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Registered: 07/29/07
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Last seen: 15 years, 4 months
Steaming And proper sterilization?
    #7814349 - 12/30/07 04:43 PM (16 years, 1 month ago)

Since my first failure at the pftek. Im going to try and do it right this time. So i dont have a pressure cooker. but i do have a semi good pot. I think veda said that you ccan use tin foil to elevate it from the bottom. but how would i do that. Without the jars flippin over? I cant use jar lids since i need the jar lids. im going to boil them for about 2 hours. Is that enough time? Or is that too much? Also when the 2hours is up. what should i do? take the pot over the stove and leave it sit over night then it would be good for inoculation? ALso how many spore syringes Do you guys think i should get for 18 jars? And wheere i think i screwed up before in my glove box. Should i put all my items in my glove box then spray lysol or should i spray before i put the items in the box. thanks


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OfflineDigitalNomad
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Re: Steaming And proper sterilization? [Re: smokeydablunt]
    #7814592 - 12/30/07 05:41 PM (16 years, 1 month ago)

Just buy some extra jars? Mason jars aren't exactly expensive.

2 hours should be fine. Just let them sit over night to insure the insides have cooled down. You don't want to go killing your spores.

I'd say two syringes. Plenty for 18 jars and a little left over to make an LC.


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Here's to the first step...


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Offlinesmokeydablunt
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Re: Steaming And proper sterilization? [Re: DigitalNomad]
    #7814667 - 12/30/07 06:04 PM (16 years, 1 month ago)

I just bought some mason jars at ace for 8 bucks for a dozen. Lc... Dont i need a pc for that? If not i would make it. I have 2 ccs left over right now.


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OfflineDigitalNomad
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Re: Steaming And proper sterilization? [Re: smokeydablunt]
    #7814684 - 12/30/07 06:09 PM (16 years, 1 month ago)

There's a good guide around here somewhere that doesn't require a PC. You only really need a PC for grains because of endospores(correct me if I'm wrong) which might survive simple heat.

You're just disinfecting water and honey in this case.
I think the tek is called LC for beginners or something? Just requires a pot. And if it works, LC is the greatest investment you'll ever make.


--------------------
Here's to the first step...


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Offlinesmokeydablunt
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Re: Steaming And proper sterilization? [Re: DigitalNomad]
    #7814705 - 12/30/07 06:15 PM (16 years, 1 month ago)

Yea but how would i sterilize all the syringes and shit? and if i could do it. How long would it take before i could use the lc.?


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OfflineDigitalNomad
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Re: Steaming And proper sterilization? [Re: smokeydablunt]
    #7814734 - 12/30/07 06:25 PM (16 years, 1 month ago)

The same way you'd normally sterilze them? I highly suggest looking up the tek.

An LC is generally done 1-2 weeks after you inject it. It will provide you with enough material to shoot up dozens of jars, that will colonize faster than if you were just using spores.

Oh, you also don't need to buy new spore syringes. Saves you money.


--------------------
Here's to the first step...


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OfflineMrAbstraction
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Re: Steaming And proper sterilization? [Re: smokeydablunt]
    #7814751 - 12/30/07 06:34 PM (16 years, 1 month ago)

To sterilize syringes;

1) Boil a sauce pan of water.
2) While waiting for that to boil, I put some rubbing alch. in a small cup, and suck it up into the syringe, and flush it back into the cup a couple of times.
3) Once the water is boiling, i remove it from the heat, and suck the water into the syringe and flush it out 5 times.
4) Leave the plunger down after the last flush.
5) Rubbing alch. the needle one last time and put the sleeve on.

Poof, sterile syringe.

/ma

EDIT: I like LC's :thumbup:


Edited by MrAbstraction (12/30/07 06:36 PM)


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OfflineDigitalNomad
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Re: Steaming And proper sterilization? [Re: MrAbstraction]
    #7814826 - 12/30/07 06:59 PM (16 years, 1 month ago)

I actually skip the water boiling stage lately, and just suck up alcohol a few times and then let it sit for a bit before shooting it out and using it. I've yet to have any problems with it at all.

That being said, your method is no doubt the proper one. :p


--------------------
Here's to the first step...


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OfflineRogerRabbitM
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Re: Steaming And proper sterilization? [Re: DigitalNomad]
    #7814876 - 12/30/07 07:16 PM (16 years, 1 month ago)

Quote:

DigitalNomad said:
Just let them sit over night to insure the insides have cooled down.  You don't want to go killing your spores.





:shrug:
When was the last time you picked up a cup of coffee when the cup was cool, but the coffee inside was still hot?  A 1/2 pint jar is even smaller than a cup of coffee, so how is that possible?  If the jar is cool to the touch, the insides are cool enough for inoculation.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
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OfflineDigitalNomad
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Re: Steaming And proper sterilization? [Re: RogerRabbit]
    #7814961 - 12/30/07 07:33 PM (16 years, 1 month ago)

Actually pretty often, seeing as I work at a coffee shop. You'd be surprised what happens there! Not to mention 105F is pretty cool for us.

Not to argue, as I know I'll just get my ass handed to me, but over night can't hurt? :p


--------------------
Here's to the first step...


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Offlinec0_hush
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Re: Steaming And proper sterilization? [Re: DigitalNomad]
    #7815697 - 12/31/07 12:54 AM (16 years, 1 month ago)

My gut tells me leaving the jars overnight increases a chance for contam exposure, specially since he's also not using a PC. When I'm done with a PC, I usually just turn off the stove and leave it alone for 5 hours while it does it's thing and then inoculate.


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Offlineihy2
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Re: Steaming And proper sterilization? [Re: smokeydablunt]
    #7815721 - 12/31/07 01:05 AM (16 years, 1 month ago)

Quote:

smokeydablunt said:
Since my first failure at the pftek.




How did you fuck up the first time? I just boiled mine for 1 hour... Everyone said I should be fine.


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Offlinesmokeydablunt
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Re: Steaming And proper sterilization? [Re: ihy2]
    #7816655 - 12/31/07 11:10 AM (16 years, 1 month ago)

I think i fucked up in my glove box. I had bought presterilized jars. I will never do that again. A stupid mistake. I thoguht it would be impossible to find jars. But all i had to do was walk down the street. Just pure laziness. Ill never be like that again. Well at least when it comes to mushroom growing.


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OfflineNibin
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Re: Steaming And proper sterilization? [Re: RogerRabbit]
    #7817029 - 12/31/07 01:09 PM (16 years, 1 month ago)

Quote:

RogerRabbit said:
Quote:

DigitalNomad said:
Just let them sit over night to insure the insides have cooled down.  You don't want to go killing your spores.





:shrug:
When was the last time you picked up a cup of coffee when the cup was cool, but the coffee inside was still hot?  A 1/2 pint jar is even smaller than a cup of coffee, so how is that possible?  If the jar is cool to the touch, the insides are cool enough for inoculation.
RR




What size cups of coffee do you drink, RR? :shocked: But then you drink that watery stuff in the US...

Oh, for 1/2 cakes put something in the bottom, be it pebbles, a dishcloth, crumpled up foil, whatever, add enough water so that it just touches the bottom half inch of the jar at most and start to boil. Once the water is boiling, lower the flame so it stays boiling but not too hard and let boil for 90 minutes, covered of course. Then or let them cool in the pot or anywhere else overnight to be safe (the foil will protect the holes), and inoculate.


--------------------
Newcomers guide-----> For all things shroomy


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Offlinesmokeydablunt
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Re: Steaming And proper sterilization? [Re: Nibin]
    #7818705 - 12/31/07 11:36 PM (16 years, 1 month ago)

My pots not big enough for 18 jars. What if i do only half at a time. Could i take the jars out and place them somewheres when i need to use the pot? or is this not safe?


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Offlineveda_sticks
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Re: Steaming And proper sterilization? [Re: smokeydablunt]
    #7818782 - 01/01/08 12:22 AM (16 years, 1 month ago)

Its best to leave the jars in the pot cooling overnight, once cool transfer to you glovebox to be inoculated


So i would sterilise your first half let cool then inoc, then do the rest of the jars, its only going to seperate them by a day maybe 2.


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek


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OfflineNibin
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Re: Steaming And proper sterilization? [Re: smokeydablunt]
    #7818840 - 01/01/08 01:11 AM (16 years, 1 month ago)

Quote:

smokeydablunt said:
My pots not big enough for 18 jars. What if i do only half at a time. Could i take the jars out and place them somewheres when i need to use the pot? or is this not safe?




It's fine. There is foil on the tops after all. Just take them out using a cloth (they will be v hot) and place them somewhere that won't get damaged by the heat (and that isn't too cold or the glass might crack).


--------------------
Newcomers guide-----> For all things shroomy


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Offlinesmokeydablunt
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Re: Steaming And proper sterilization? [Re: Nibin]
    #7820061 - 01/01/08 01:27 PM (16 years, 1 month ago)

I guess ill just set in my incubator without it being on or anything. Would that be ideal? Ill clean it out. With alcohol. thanks


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Offlineveda_sticks
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Re: Steaming And proper sterilization? [Re: smokeydablunt]
    #7820081 - 01/01/08 01:33 PM (16 years, 1 month ago)

I just remembered that i think it was RR mentioned using perlite on the bottom of the pot to get the jars of the bottom???

Jar lids work best if you have lots of spares, you can buy them pretty cheap.

The couple times i steam sterilised i used a cloth on the bottom of the pot.


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek


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Offlinesmokeydablunt
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Re: Steaming And proper sterilization? [Re: veda_sticks]
    #7820116 - 01/01/08 01:45 PM (16 years, 1 month ago)

DId it work out just using cloth?


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