|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
MYSTIQUE
Say Hi to the elves for me.




Registered: 04/28/07
Posts: 1,764
Loc: Canada
Last seen: 5 years, 4 months
|
Idea on inoculation
#7809619 - 12/29/07 02:22 AM (16 years, 1 month ago) |
|
|
OK I was trying to think of a way to stop contamination and maybe even not need a glove box during inoculation or at least add to the still air boxes allot of us use when we don't have the money for the glove box. Its not a totally new idea but just improved some.
The idea is to take a glass funnel just a bit larger then a syringe. one of the longer ones. Ill try and find a example. You then file the end of it just a little to sharpen it. Well after you have the funnel sharp you shove a piece of sponge down into the thin part of the funnel but not so small it comes out the other end when its pushed on.
Now the whole thing is made how to use it. First You take some 99%ISO and pour it into the funnel and the sponge should hold a little at the top. Next push it down into your silicon injecting site and twist just a little to make a seal with the sharp end but don't cut to deep. Then heat the syringe and push it threw the small pool of ISO to cool and threw the sponge into the injection site.
Here is a little picture 1. Glass funnel 2. ISO layer 3. Sponge 4. syringe 5. silicone
[image] [/image]
-------------------- Dont know what the fuck I just said? READ THIS http://www.shroomery.org/5122/The-Shroomery-Mushroom-Glossary I ain't a hippy but I'm covered in dirt Sippin lots of mushroom tea in a tye-dye shirt Chasin' the Grateful Dead, no shoes on my feet Beggin' in the parking lot for something to eat, DO NOT USE FIRE IN YOUR GLOVE BOX!!!!!!!
|
kupa trooper



Registered: 11/11/07
Posts: 750
Loc: Earth
|
Re: Idea on inoculation [Re: MYSTIQUE]
#7809636 - 12/29/07 02:40 AM (16 years, 1 month ago) |
|
|
what is your sig from or did you make it up? sorry it off topic
-------------------- I don't know but I've been told. It's hard to run with the weight of gold On the other hand I heard it said. It's just as hard with the weight of lead.
Edited by kupa trooper (12/29/07 02:41 AM)
|
CantiSama


Registered: 10/21/04
Posts: 794
Last seen: 4 months, 10 days
|
Re: Idea on inoculation [Re: MYSTIQUE]
#7809699 - 12/29/07 03:23 AM (16 years, 1 month ago) |
|
|
you use a glovebox when you inoculate a jar with a syringe? that seems a little excessive.
--------------------
Tiger got to hunt, bird got to fly; Man got to sit and wonder 'why, why, why?' Tiger got to sleep, bird got to land; Man got to tell himself he understand.
|
Nibin
Getting there



Registered: 11/29/05
Posts: 4,480
Last seen: 10 years, 8 months
|
Re: Idea on inoculation [Re: MYSTIQUE]
#7811300 - 12/29/07 06:20 PM (16 years, 1 month ago) |
|
|
Quote:
MYSTIQUE said: OK I was trying to think of a way to stop contamination and maybe even not need a glove box during inoculation or at least add to the still air boxes allot of us use when we don't have the money for the glove box. Its not a totally new idea but just improved some.
The idea is to take a glass funnel just a bit larger then a syringe. one of the longer ones. Ill try and find a example. You then file the end of it just a little to sharpen it. Well after you have the funnel sharp you shove a piece of sponge down into the thin part of the funnel but not so small it comes out the other end when its pushed on.
Now the whole thing is made how to use it. First You take some 99%ISO and pour it into the funnel and the sponge should hold a little at the top. Next push it down into your silicon injecting site and twist just a little to make a seal with the sharp end but don't cut to deep. Then heat the syringe and push it threw the small pool of ISO to cool and threw the sponge into the injection site.
Here is a little picture 1. Glass funnel 2. ISO layer 3. Sponge 4. syringe 5. silicone
[image] [/image]
Reminds me of Agar's needle condoms.
Also use 70% ISO not 99%, it kills contaminants faster.
-------------------- Newcomers guide-----> For all things shroomy
Edited by Nibin (12/29/07 06:21 PM)
|
veda_sticks
Cultivator




Registered: 07/29/07
Posts: 14,191
Loc: UK
Last seen: 4 years, 25 days
|
Re: Idea on inoculation [Re: Nibin]
#7811328 - 12/29/07 06:29 PM (16 years, 1 month ago) |
|
|
Its a nice idea but a little overkill, a peice of sponge bigger than the needle soaked in alcohol works well, acts as a retracting sleeve.
This idea has been about for a while, adding the glass funnel i would imagine wouldnt make that much of a difference. A simple glove box has had 100% success for me, didnt cost any money as i had an unused clear box.
I think it would probably cost jsut about as much to have a glass funnel made to fit the syringe as making a glovebox.
-------------------- PF TEK - writeup by EvilMushroom666 Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD Cakes can and will pin! - So you think cakes suck for pins. Your wrong Franks Simple Coir/Verm Tek Franks Proper Pasturisation Tek Franks Spawning To Bulk - Monotub Professor Pinheads RTV Injection Port Tek Foo Mans No Soak WBS Prep Tek
|
MYSTIQUE
Say Hi to the elves for me.




Registered: 04/28/07
Posts: 1,764
Loc: Canada
Last seen: 5 years, 4 months
|
|
Lol I'm really afraid of needles I cant use those sponge selves. I'm afraid it will go into my finger when I cant see it lol. I will soon move to agar just the availability of it to Canada and the cost of petri dishes , shipping it all sucks.
-------------------- Dont know what the fuck I just said? READ THIS http://www.shroomery.org/5122/The-Shroomery-Mushroom-Glossary I ain't a hippy but I'm covered in dirt Sippin lots of mushroom tea in a tye-dye shirt Chasin' the Grateful Dead, no shoes on my feet Beggin' in the parking lot for something to eat, DO NOT USE FIRE IN YOUR GLOVE BOX!!!!!!!
|
Funkatron9000
Astronaut



Registered: 10/03/07
Posts: 535
Loc: Sector Zed: Region 6: Sol...
Last seen: 2 years, 2 months
|
Re: Idea on inoculation [Re: MYSTIQUE]
#7811428 - 12/29/07 07:03 PM (16 years, 1 month ago) |
|
|
Flame and ISO the needle and everything should be fine, if you have any problems after that try using tyvek under the lid.
-------------------- You cannot yet touch my daughters awesome boobage
You must fight to the death in the Breastriary of Nippopolis.
I thought Gene Wilder was cool BEFORE he was dead.
|
|
|
You cannot start new topics / You cannot reply to topics HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a 476 topic views. 18 members, 175 guests and 51 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ] |
|