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veda_sticks
Cultivator




Registered: 07/29/07
Posts: 14,191
Loc: UK
Last seen: 4 years, 25 days
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If you only waited an hour before inoculating, the temps inside the jar could have been way above the thermal deathpoint of spores.
Most likely your spores have died. And the high incubation temp has helped the bacteria.
Nibin is right, even when pressure cooking u cant guarentee complete sterility. There will always be a few endospores that survive. What we are aiming to do is give the mycelium the best chance at surviving against other organisms that may have survived. Myc growth starts to slow down above around 83F ambient temperature.
Even whe we pressure cook, there just might be that 1 or 2 that make it.
-------------------- PF TEK - writeup by EvilMushroom666 Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD Cakes can and will pin! - So you think cakes suck for pins. Your wrong Franks Simple Coir/Verm Tek Franks Proper Pasturisation Tek Franks Spawning To Bulk - Monotub Professor Pinheads RTV Injection Port Tek Foo Mans No Soak WBS Prep Tek
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doc34
Fungitarian



Registered: 02/14/04
Posts: 2,667
Loc: Myceliaville !!!
Last seen: 7 months, 29 days
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Quote:
veda_sticks said: If you only waited an hour before inoculating, the temps inside the jar could have been way above the thermal deathpoint of spores.
Most likely your spores have died. And the high incubation temp has helped the bacteria.
Nibin is right, even when pressure cooking u cant guarentee complete sterility. There will always be a few endospores that survive. What we are aiming to do is give the mycelium the best chance at surviving against other organisms that may have survived. Myc growth starts to slow down above around 83F ambient temperature.
Even whe we pressure cook, there just might be that 1 or 2 that make it.
DITTO!
1 hour wait is not enuf. You should wait @ least 10-12 hours to make sure the center of your jars are cool enuf to inoculate-especially quarts. You might have pulled it off with 1/2 pints due to their size and the small amount of substrate, but not quarts-I wait overnight(10-24 hours). Patience is the key to success in cultivation.
My two cents
Doc
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anarchOi
Ellenalien is fat.



Registered: 08/06/07
Posts: 2,293
Loc: ASE
Last seen: 12 years, 11 months
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Re: Whats that smell? [Re: doc34]
#7816889 - 12/31/07 12:22 PM (16 years, 1 month ago) |
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your incubators cleanliness shouldn't even be an issue
you don't innoc in your incubator and your jars are supposed to be sealed except for filtered air exchanges maybe i'm crazy but i still use tyvek, duct tape for the innoc holes and then tinfoil (in the instance of a cake, all of that along with a verm barrier) i've never had a cake contam in my incubator, and the rye seed that contam'd was expected after a PC problem none of the contams ever spread from jar to jar so i'm guessing it'd be pretty hard for contams to get in OR out of my jars without error of my own
how much sense does it make to do away with things that work well just because people fail to do it right? i.e not incubating because you can't inoculate with contaminating
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Edited by anarchOi (12/31/07 12:24 PM)
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Dephect




Registered: 08/10/07
Posts: 1,779
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Re: Whats that smell? [Re: anarchOi]
#7816935 - 12/31/07 12:37 PM (16 years, 1 month ago) |
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actually the heat of the jar should be no problem... in my LSD haze from christmas I remember that my syrienge came a day after i boiled my jars and i knocked them up the day after..
but i do need to work on being sterile, getting a PC and not overheating my jars which is what i think killed the mycellum that was growing in there and gave climate to a bacteria because those jars have by far the worst smell i have ever breathed in.. i cant even stand it without gagging..
there was white stuff, mycellum i think growing in the jar before it died.. thanks for the input everyone my Rye Grain jar is on the way!
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Nibin
Getting there



Registered: 11/29/05
Posts: 4,480
Last seen: 10 years, 8 months
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Re: Whats that smell? [Re: Dephect]
#7816977 - 12/31/07 12:49 PM (16 years, 1 month ago) |
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Quote:
Dephect said: Okay.. So this is kind of off topic but say you created a account with a Shroomery vendor under the wrong email and did not realize it.. Mycopath to be exact.. and say you order a few items that you really want and realize at the last minute your email is wrong and you dont get a email telling you that you bought them but your order is still processed..
Oh man guys what should I do?
All the shipping info was right do you think I just have to wait for it without knowing where it is?
Email them with the order info and shipping address and tell them the email address was wrong.
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Dephect




Registered: 08/10/07
Posts: 1,779
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Re: Whats that smell? [Re: Nibin]
#7817017 - 12/31/07 01:04 PM (16 years, 1 month ago) |
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I called the number today.. He said he couldnt change my email on the order but he would forward a copy of the order to my correct email.. Man I love MycoPath and their wonderful customer service.. Thanks Graham!
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