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Offlinebeeplus
Stranger
Registered: 11/26/07
Posts: 56
Last seen: 12 years, 11 months
contams, hooray!
    #7804377 - 12/27/07 03:05 PM (16 years, 1 month ago)

I have tried to make 3 different LC's 3 different ways, and somehow i have managed to get contams in every single one.

1) GT spore syringe -- contam
2) WBS sample -- contam
3) BRF sample -- contam

They were all done at different times. I took a shower before all of them and I brushed teeth, used mouthwash, etc etc in a makeshift glovebox.

I don't want to buy another syringe, what should I do? I threw out my WBS and my BRF cake. Right now I have an empty syringe, and some empty half pints, along with 6 mycobags that i need to make an LC for.

Someone please point me in the right direction.


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InvisibleDephect
Male User Gallery


Folding@home Statistics
Registered: 08/10/07
Posts: 1,779
Re: contams, hooray! [Re: beeplus]
    #7804389 - 12/27/07 03:10 PM (16 years, 1 month ago)

My first try didnt even colonize (yet?)
Just keep trying.. Look for some free prints and make a new syrienge..
This time I learned from my mistakes and I am ordering a 1/2 Gallon Rye Berry Jar from MycoPath.. If you do it right its guaranteed sterile and contam free..

Keep trying mate!
Dont give up!


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Offlinebeeplus
Stranger
Registered: 11/26/07
Posts: 56
Last seen: 12 years, 11 months
Re: contams, hooray! [Re: Dephect]
    #7804412 - 12/27/07 03:22 PM (16 years, 1 month ago)

If I really have to, I will certainly buy a syringe. Is there a FSR for America?

Thanks for the help.


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OfflineNibin
Getting there
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Registered: 11/29/05
Posts: 4,480
Last seen: 10 years, 8 months
Trusted Cultivator
Re: contams, hooray! [Re: beeplus]
    #7804608 - 12/27/07 04:33 PM (16 years, 1 month ago)

There is one in Canada.


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Offlineverminute
Stay out of theOTD!
Male

Registered: 11/16/07
Posts: 595
Last seen: 15 years, 10 months
Re: contams, hooray! [Re: Nibin]
    #7804792 - 12/27/07 05:22 PM (16 years, 1 month ago)

Get lysol wipes that advertise "kills the flu". Clean the fuck out of everything near. Then invest in purell and use on your mitts after every new touch. Good luck.

My first go I got contaim in 2 out of 12 jars. Mostly because I inculated way too much... But then again I'm a clean freak, and I don't touch the jars everyday.


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Invisibledumbfounded1600
Stranger

Registered: 07/29/07
Posts: 2,624
Re: contams, hooray! [Re: verminute]
    #7804869 - 12/27/07 05:44 PM (16 years, 1 month ago)

this is the reason people don't like to do LC's because it contams very easy and theres no way of telling if its contammed other then try it out on a test jar..SO....stick to agar


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Invisiblethedefone
deus ex machina

Registered: 10/06/07
Posts: 1,883
Loc: Gondwana
Re: contams, hooray! [Re: dumbfounded1600]
    #7805592 - 12/27/07 09:18 PM (16 years, 1 month ago)

Sporeworks has cube syringes for 10$ as long as you don't care what strain it is. No fresh fruits lying around? Colonized jars, maybe?


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I am become death, the destroyer of worlds.


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