|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
mushsage
Stranger
Registered: 10/27/07
Posts: 22
Last seen: 15 years, 10 months
|
how many jars can a 60mm petri culture do?
#7802910 - 12/27/07 03:55 AM (16 years, 1 month ago) |
|
|
i'm new to using cultures and i was wondering how many quart jars i can inoculate(is that the correct word if you use cultures?) with a fully colonized 60x15mm petri dish
thanks!
|
RRVideo
Stranger



Registered: 05/18/07
Posts: 106
Loc: Wilderness cabin
Last seen: 15 years, 1 month
|
Re: how many jars can a 60mm petri culture do? [Re: mushsage]
#7803131 - 12/27/07 07:50 AM (16 years, 1 month ago) |
|
|
Personally, I'd just quarter it and use each plate to do four jars of grain. I just drop the wedge right on top of the grains and don't shake. This way, the mycelium recovers faster into the grains, and then you can shake on about day three or four, and the jar is finished between days 7 and 10. RR
|
Leonidas25
Stranger


Registered: 06/16/07
Posts: 85
Last seen: 15 years, 11 months
|
Re: how many jars can a 60mm petri culture do? [Re: mushsage]
#7803169 - 12/27/07 08:13 AM (16 years, 1 month ago) |
|
|
I like to take a fully colonized (healthy and contam free) petri dish and make a liquid suspension syringe of fragmented mushroom mycelium via the good ole eberbach container... This way you can inoculate many more jars than the conventional way of transferring agar wedges. Also when you inoculate, the liquid acts as a transport mechanism letting all the little fragments of mycelium trickle all the way down through the jars. This way there are a ton of inoculation points instead of just one...just be sure not to add too much or you'll screw up the moisture content of the jars...
|
somebody041
bud tester


Registered: 06/04/07
Posts: 476
Loc: California
Last seen: 9 years, 9 months
|
Re: how many jars can a 60mm petri culture do? [Re: Leonidas25]
#7804099 - 12/27/07 01:40 PM (16 years, 1 month ago) |
|
|
Quote:
Leonidas25 said: I like to take a fully colonized (healthy and contam free) petri dish and make a liquid suspension syringe of fragmented mushroom mycelium via the good ole eberbach container... This way you can inoculate many more jars than the conventional way of transferring agar wedges. Also when you inoculate, the liquid acts as a transport mechanism letting all the little fragments of mycelium trickle all the way down through the jars. This way there are a ton of inoculation points instead of just one...just be sure not to add too much or you'll screw up the moisture content of the jars...
could you elaborate on the process of how to make a liquid suspension syringe?
|
Premedman1
Assistant to the insistent



Registered: 12/21/05
Posts: 2,376
Loc: South of Sanity
|
Re: how many jars can a 60mm petri culture do? [Re: somebody041]
#7804125 - 12/27/07 01:45 PM (16 years, 1 month ago) |
|
|
Try a search for "Liquid Culture".
-------------------- Build a man a fire, he is warm for the night. Set a man on fire, he is warm for the rest of his life.
|
somebody041
bud tester


Registered: 06/04/07
Posts: 476
Loc: California
Last seen: 9 years, 9 months
|
Re: how many jars can a 60mm petri culture do? [Re: Premedman1]
#7804208 - 12/27/07 02:06 PM (16 years, 1 month ago) |
|
|
ahh, i did not know that the two terms meant the same thing
|
Leonidas25
Stranger


Registered: 06/16/07
Posts: 85
Last seen: 15 years, 11 months
|
Re: how many jars can a 60mm petri culture do? [Re: somebody041]
#7804237 - 12/27/07 02:13 PM (16 years, 1 month ago) |
|
|
could you elaborate on the process of how to make a liquid suspension syringe?
Well what I do is fill the eberbach container about 2/3 full with water, place the lid on, cover with aluminum foil, and pressure cook for 30 min @15 psi. Allow to cool to room temp ( I let it cool off in front of my flow hood ). Then in front of a flow hood or in a glove box using a flame sterilized scalpel slice up a healthy contam free petri dish and place the agar wedges into the eberbach, close the lid and blend it up for about 3-5 seconds. Open the eberbach in front of the hood again or in the glove box and suck up the solution with a sterile needle/syringe...
I have made 140cc syringes like this, used only half and saved the rest in the syringe in a refrigerator, 3 months later again used the remaining 70cc of solution and it worked just fine...
|
|
|
You cannot start new topics / You cannot reply to topics HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a 1,089 topic views. 17 members, 140 guests and 40 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ] |
|