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OfflineSkinWalker
COW WITH HUMANHEAD


Registered: 12/25/07
Posts: 42
Last seen: 16 years, 1 month
Old LC in tha fridge
    #7798962 - 12/25/07 06:20 PM (16 years, 1 month ago)

Hello, im wanting to start another new grow. The last one was cut short for some reasons.
I have alot of left over spores and about 10ccs of LC.
the thing is that its pretty old. about 10 months old.
I was wondering if i could still use it. Im planning on knocking up 12 half pint jars? do i have enough?

But the main question is if it is too old. I stored it in a air tight zip lock bag in the bottom of my fridge.
And also i looked at it and it looks like it has a like 2 clumps of black.. Im not sure if its a contamn or spores. I think its spores because i used a empty spore syringe and you know how spores usualy stick to the bottom of the syringe?
Would it be safe if i use it? Contamns usualy wont grow in a cold ass fridge would they?
Ok thank you guys.


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OfflineSkinWalker
COW WITH HUMANHEAD


Registered: 12/25/07
Posts: 42
Last seen: 16 years, 1 month
Re: Old LC in tha fridge [Re: SkinWalker]
    #7799118 - 12/25/07 07:29 PM (16 years, 1 month ago)

please help


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OfflineSkinWalker
COW WITH HUMANHEAD


Registered: 12/25/07
Posts: 42
Last seen: 16 years, 1 month
Re: Old LC in tha fridge [Re: SkinWalker]
    #7799190 - 12/25/07 07:56 PM (16 years, 1 month ago)

bump


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InvisibleAtheist
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Registered: 01/24/06
Posts: 13,705
Loc: USA
Re: Old LC in tha fridge [Re: SkinWalker]
    #7799192 - 12/25/07 07:57 PM (16 years, 1 month ago)

if it has 2 black chunks i doubt it is still good


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OfflineSkinWalker
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Registered: 12/25/07
Posts: 42
Last seen: 16 years, 1 month
Re: Old LC in tha fridge [Re: Atheist]
    #7799200 - 12/25/07 07:59 PM (16 years, 1 month ago)

not chunks.. more like small flakes. like how the spores look in the syringe.
my main concern is if the myc is still healthy after that amount of time.


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InvisibleAtheist
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Re: Old LC in tha fridge [Re: SkinWalker]
    #7799210 - 12/25/07 08:01 PM (16 years, 1 month ago)

it could be a black mold but i dont know

a good way to find out is to test it on a jar


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OfflineSkinWalker
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Registered: 12/25/07
Posts: 42
Last seen: 16 years, 1 month
Re: Old LC in tha fridge [Re: Atheist]
    #7799217 - 12/25/07 08:05 PM (16 years, 1 month ago)

black mold cant grow in those cold conditions can it? if anything i would think bacterial contams would be my problem.


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Invisiblestinkbuttdog
I scat.


Registered: 04/08/07
Posts: 514
Loc: In the zone, cabrone!
Re: Old LC in tha fridge [Re: SkinWalker]
    #7799238 - 12/25/07 08:13 PM (16 years, 1 month ago)

LINK

Bing bodda boom! Stinkbutt to the rescue!


:matrix2:  kickin ass one question at a time!


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OfflineTwiggs
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Registered: 11/14/06
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Re: Old LC in tha fridge [Re: SkinWalker]
    #7799241 - 12/25/07 08:14 PM (16 years, 1 month ago)

if its only 10 cc's then it would be worth taking your spores and making 200 cc's worth


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InvisibleFooMan
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Folding@home Statistics
Registered: 02/02/05
Posts: 8,957
Loc: Earth Flag
Trusted Cultivator
Re: Old LC in tha fridge [Re: SkinWalker]
    #7799277 - 12/25/07 08:32 PM (16 years, 1 month ago)

How is anyone going to be able to tell you what the black stuff at the bottom of your lc jar is? Secondly, you said you've got a 10 month old LC, so you've waited 10 months since inoculating it. Why are you in such a the rush now that you've bumped this post twice in a few hours? Test the LC and spores on small batches of substrate, then proceed from there. It's not rocket science.


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Quick WBS Prep


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OfflineSkinWalker
COW WITH HUMANHEAD


Registered: 12/25/07
Posts: 42
Last seen: 16 years, 1 month
Re: Old LC in tha fridge [Re: FooMan]
    #7799282 - 12/25/07 08:35 PM (16 years, 1 month ago)

just answer the question.

IS A 10 MONTH OLD LC STILL VIABLE


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Invisiblestinkbuttdog
I scat.


Registered: 04/08/07
Posts: 514
Loc: In the zone, cabrone!
Re: Old LC in tha fridge [Re: FooMan]
    #7799284 - 12/25/07 08:36 PM (16 years, 1 month ago)

Now THAT'S an angry pussy if ever I've seen one!


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Invisiblestinkbuttdog
I scat.


Registered: 04/08/07
Posts: 514
Loc: In the zone, cabrone!
Re: Old LC in tha fridge [Re: stinkbuttdog]
    #7799289 - 12/25/07 08:37 PM (16 years, 1 month ago)

Quote:

stinkbuttdog said:
LINK

Bing bodda boom! Stinkbutt to the rescue!


:matrix2:  kickin ass one question at a time!




HERE IS THE ALL KNOWING ANSWER TO YOUR SPECIFIC QUESTION...

just hit the link and go from there... sheesh. Bossy!


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InvisibleFooMan
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Re: Old LC in tha fridge [Re: SkinWalker]
    #7799308 - 12/25/07 08:48 PM (16 years, 1 month ago)

The magic 8-ball says- How in the hell is anyone going to be able to tell you if your LC is viable? TRY IT and see.


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Quick WBS Prep


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OfflineAlphanautics
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Male


Registered: 11/26/07
Posts: 103
Last seen: 4 years, 25 days
Re: Old LC in tha fridge [Re: FooMan]
    #7799887 - 12/26/07 12:44 AM (16 years, 1 month ago)

hey what is your LC in? not that id be the guy to answer this, but i've heard distilled water, w. myc suspended in it, with the proper technique obviously, is money.... years and years allegedly... why dont u just try it.. birdseed's not THAT expensive, now...

or agar up


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Offlineveda_sticks
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Re: Old LC in tha fridge [Re: Alphanautics]
    #7800122 - 12/26/07 05:12 AM (16 years, 1 month ago)

A liquid culture can be kept for many years in the refrigorator. Many growers use this method of having a master culture, which they use to make up more lc's which they use to grow.

When they run out of LC they go back to there master and use that to inoculate more LC.

A simple use of the search would have found your answer.

Yes a refrigerated 10 month LC can still be viable.

However i am unsure about the black in the syringe, could be spores. who knows.

Make up pf jar and test it out on that


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